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pX330-syn-crRNA-TS-sgRNA

Expression vector of Cas9 and guide RNA in order to excise the targeting cassette cloned in the pCriMGET_v1 vector (RDB19318).

Catalog number RDB19319
Resource name pX330-syn-crRNA-TS-sgRNA
Clone info. Expression vector of Cas9 and guide RNA in order to excise the targeting cassette cloned in the pCriMGET_v1 vector (RDB19318). See Fig.4 and Fig.S2 of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Ampicillin
Gene/insert name S. pyogenes Cas9 genomic
Depositor|Developer Ishibashi, Riki |
 
Sequence (full) RDB19319hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19319_B1Jep1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
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Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
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提供依頼書 [Word]
専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19319 pX330-syn-crRNA-TS-sgRNA DNA solution


How to cite this biological resource

Materials & Methods section:

The pX330-syn-crRNA-TS-sgRNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19319).

Reference section:

Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., Toyoshima, F., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]