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pX330-syn-crRNA-TS-sgRNA (#RDB19319)

Expression vector of Cas9 and guide RNA in order to excise the targeting cassette cloned in the pCriMGET_v1 vector (RDB19318).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Ishibashi, R., Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of Cas9 and guide RNA in order to excise the targeting cassette cloned in the pCriMGET_v1 vector (RDB19318). See Fig.4 and Fig.S2 of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name S. pyogenes Cas9 genomic
Depositor|Developer Ishibashi, Riki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee
RDB19319 pX330-syn-crRNA-TS-sgRNA DNA solution

How to cite this biological resource

Materials & Methods section:

The pX330-syn-crRNA-TS-sgRNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19319).

Reference section:

Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., Toyoshima, F., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [PubMed] [Article] [RRC of NBRP]