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pX330-syn-crRNA-TS-sgRNA

Expression vector of Cas9 and guide RNA in order to excise the targeting cassette cloned in the pCriMGET_v1 vector (RDB19318).

Catalog number RDB19319
Resource name pX330-syn-crRNA-TS-sgRNA
Clone info. Expression vector of Cas9 and guide RNA in order to excise the targeting cassette cloned in the pCriMGET_v1 vector (RDB19318). See Fig.4 and Fig.S2 of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Ampicillin
Gene/insert name S. pyogenes Cas9 genomic
Depositor|Developer Ishibashi, Riki |
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External Database
S. pyogenes Cas9

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          備考
          本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をご参照ください。

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          RDB19319 pX330-syn-crRNA-TS-sgRNA Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482.

          2022.03.21

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