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pCriMGET_v1-AAVS1-SA-Neo-pA

Targeting construct to insert Splicing Acceptor (SA)-neomycin resistance gene (neo)-bGHpA into the human AAVS1 locus by the homologous recombination.

Catalog number RDB19320
Resource name pCriMGET_v1-AAVS1-SA-Neo-pA
Clone info. AAVS1 targeting construct to insert Splicing Acceptor (SA)-neomycin resistance gene (neo)-bGHpA into the human AAVS1 locus by the homologous recombination. This clone was used with pX330-AAVS1-sgRNA (RDB19323) and pX330-syn-crRNA-TS-sgRNA (RDB19319) in Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pBluescriptII SK(+) (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name human AAVS1 genomic
Depositor|Developer Ishibashi, Riki |
Other clones in our bank
External Database
human AAVS1
          Reference sequence
            
           
          Sequence (full) RDB19320hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19320_B1Jep1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
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          RDB19320 pCriMGET_v1-AAVS1-SA-Neo-pA DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pCriMGET_v1-AAVS1-SA-Neo-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19320).

          Reference section:

          Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., Toyoshima, F., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

          2023.12.26

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