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pCriMGET_v1

Plasmid vector to clone a targeting cassette. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo.

Catalog number RDB19318
Resource name pCriMGET_v1
Clone info. Plasmid vector to clone a targeting cassette. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo. See Fig.1 of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pBluescriptII SK(+) (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name synthetic poly(A) site (SPA).
Depositor|Developer Ishibashi, Riki |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB19318hts01.seqcheck assembled from experimentally sequenced data
            Test sheet RDB19318_B1Jep1-1.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
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            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19318 pCriMGET_v1 DNA solution


            How to cite this biological resource

            Materials & Methods section:

            The pCriMGET_v1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19318).

            Reference section:

            Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., Toyoshima, F., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            References

            Original, user report and related articles

            original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

            2023.12.26

            GNP_filter3_RDBDEP_html_231214.pl