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pCriMGET_v1-Tbx3-3xFlag-2A-EGFP

Targeting construct to insert 3xFlag-P2A-EGFP into flanked stop codon of mouse Tbx3 gene.

Catalog number RDB19321
Resource name pCriMGET_v1-Tbx3-3xFlag-2A-EGFP
Clone info. Tbx3 targeting construct to insert 3xFlag-P2A-EGFP into flanked stop codon of mouse Tbx3 gene. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo. See Fig.6A and 6B of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pBluescriptII SK(+) (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Tbx3 genomic
Depositor|Developer Ishibashi, Riki |
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External Database
mouse Tbx3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Schedule A (GFP-A) [Link]
          Remarks
          Please use Schedule A (GFP-A) additionally.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          Schedule A(GFP-A, 英語版のみ) [Link]
          備考
          Schedule A(GFP-A, 英語版のみ)が追加で必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19321 pCriMGET_v1-Tbx3-3xFlag-2A-EGFP Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482.

          2022.03.21

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