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pCriMGET_v1-Tbx3-3xFlag-2A-EGFP

Targeting construct to insert 3xFlag-P2A-EGFP into flanked stop codon of mouse Tbx3 gene.

Catalog number RDB19321
Resource name pCriMGET_v1-Tbx3-3xFlag-2A-EGFP
Clone info. Tbx3 targeting construct to insert 3xFlag-P2A-EGFP into flanked stop codon of mouse Tbx3 gene. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo. See Fig.6A and 6B of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pBluescriptII SK(+) (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Tbx3 genomic
Depositor|Developer Ishibashi, Riki |
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External Database
mouse Tbx3

          Reference sequence
            
           
          Sequence (full) RDB19321hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19321_B1Jep1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19321 pCriMGET_v1-Tbx3-3xFlag-2A-EGFP DNA solution


          How to cite this biological resource

          Materials & Methods section:

          The pCriMGET_v1-Tbx3-3xFlag-2A-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19321).

          Reference section:

          Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., Toyoshima, F., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          References

          Original, user report and related articles

          original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

          2023.12.26

          GNP_filter3_RDBDEP_html_231214.pl