Resource data sheet
DNA Bank Top

pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP (#RDB19322)

Targeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format
SnapGene file
Publication Ishibashi, R., Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]
Test sheet Data Sheet 
 
Clone info. Clec4f targeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo. See Fig.S8A and S8B of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pBluescriptII SK(+) (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name mouse Clec4f genomic human HBEGF
Depositor|Developer Ishibashi, Riki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。

Catalog # Resource name Shipping form Fee
RDB19322 pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP DNA solution

How to cite this biological resource

Materials & Methods section:

The pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19322).

Reference section:

Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., Toyoshima, F., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]