Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP

Targeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene.

Catalog number RDB19322
Resource name pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP
Clone info. Clec4f targeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo. See Fig.S8A and S8B of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pBluescriptII SK(+) (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Clec4f genomic human HBEGF
Depositor|Developer Ishibashi, Riki |
 
Sequence (full) RDB19322hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19322_B1Jep1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
Ordering
Order form [Credit Card Payment]  [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19322 pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP DNA solution


How to cite this biological resource

Materials & Methods section:

The pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19322).

Reference section:

Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., Toyoshima, F., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]