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pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP

Targeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene.

Catalog number RDB19322
Resource name pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP
Clone info. Clec4f targeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo. See Fig.S8A and S8B of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pBluescriptII SK(+) (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Clec4f genomic human HBEGF
Depositor|Developer Ishibashi, Riki |
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External Database
mouse Clec4f human HBEGF

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Schedule A (GFP-A) [Link]
        Remarks
        Please use Schedule A (GFP-A) additionally.
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
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        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        Schedule A(GFP-A, 英語版のみ) [Link]
        備考
        Schedule A(GFP-A, 英語版のみ)が追加で必要です。

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        RDB19322 pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Electronic file

        Original reference

        original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482.

        Further references such as user reports and related articles (go to bottom)

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        Sequence information

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482.

        2022.03.21

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