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Shigeru Makino's web page in English / in Japanese
| Catalog # | Name of clone | Running title | Publication |
| RDB15042 | pCMV-3xFLAG-mGli3_insGafter97G-HA | Expression vector of mouse mutant Gli3, one-base-pair insertion of G after 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed. | Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB15041 | pCMV-3xFLAG-mGli3_del97G-HA | Expression vector of mouse mutant Gli3, one-base-pair deletion of 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed. | Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB15040 | pCMV-3xFLAG-mGli3_WT-HA | Expression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed. | Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB15039 | pCMV-3xFLAG-mGli3_WT | Expression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG. Though this clone was constructed based on the pEGFP-C1, GFP was removed. | Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB15038 | pSpCas9-mGli3-exon3_sgRNA | Expression vector of sgRNA for targeting mouse Gli3-exon3. | Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB15037 | pSpCas9-mGli3-exon2_sgRNA | Expression vector of sgRNA for targeting mouse Gli3-exon2. | Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [PubMed] [Article] [RRC of NBRP] (original) |
2026.06.04
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