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Shigeru Makino's Resource

Shigeru Makino's web page in English    in Japanese.

Learn more about their research in Reseachmap[link]   in English     in Japanese.

Catalog #Name of cloneRunning titlePublication
RDB15037pSpCas9-mGli3-exon2_sgRNAExpression vector of sgRNA for targeting mouse Gli3-exon2.Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. (original).
RDB15038pSpCas9-mGli3-exon3_sgRNAExpression vector of sgRNA for targeting mouse Gli3-exon3.Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. (original).
RDB15039pCMV-3xFLAG-mGli3_WTExpression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG. Though this clone was constructed based on the pEGFP-C1, GFP was removed.Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. (original).
RDB15040pCMV-3xFLAG-mGli3_WT-HAExpression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. (original).
RDB15041pCMV-3xFLAG-mGli3_del97G-HAExpression vector of mouse mutant Gli3, one-base-pair deletion of 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. (original).
RDB15042pCMV-3xFLAG-mGli3_insGafter97G-HAExpression vector of mouse mutant Gli3, one-base-pair insertion of G after 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. (original).

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2019.07.19

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