Resource data sheet
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pCMV-3xFLAG-mGli3_insGafter97G-HA (#RDB15042)

Expression vector of mouse mutant Gli3, one-base-pair insertion of G after 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.

Clone info. Expression vector of mouse mutant Gli3, one-base-pair insertion of G after 97G, tagged with N-terminal 3xFLAG and C-terminal HA, see Fig3A of Makino, S. et al. Sci. Rep., 6: 39608, 2016.
Comment Expression was confirmed by the depositor with direct sequencing and western blotting.
Vector backbone
pEFGP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Gli3 cDNA
Depositor|Developer Gondo, Yoichi | Makino, Shigeru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino,S. et al. Sci. Rep., 6: 39608, 2016).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino,S. et al. Sci. Rep., 6: 39608, 2016)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15042 pCMV-3xFLAG-mGli3_insGafter97G-HA Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCMV-3xFLAG-mGli3_insGafter97G-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15042).

Reference section:

Makino, S., Fukumura, R., Gondo ,Y., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [link to RRC of NBRP]