RIKEN DNA Bank Mouse Resource

RIKEN DNA Bank Mouse Resource - Gli3

Gene ID	NCBI Gene 14634 \| KEGG mmu:14634
Gene Symbol	Gli3
Protein Name	GLI-Kruppel family member GLI3
Synonyms	Bph\|GLI3-190\|GLI3FL\|Pdn\|Xt\|add
Featured content	Hedgehog signaling (mouse)

Link

Ortholog resource in our bank

Gli3

External dsatabase

mouse Gli3

Individualy Deposited Resource

Catalog number	Name of Resource	Description	CDS comparison
Catalog number	Name of Resource	Description	Refered (NCBI mRNA)	CDS status⁽¹⁾
RDB15042	pCMV-3xFLAG-mGli3_insGafter97G-HA	Expression vector of mouse mutant Gli3, one-base-pair insertion of G after 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.
RDB15041	pCMV-3xFLAG-mGli3_del97G-HA	Expression vector of mouse mutant Gli3, one-base-pair deletion of 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.
RDB15040	pCMV-3xFLAG-mGli3_WT-HA	Expression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.
RDB15039	pCMV-3xFLAG-mGli3_WT	Expression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG. Though this clone was constructed based on the pEGFP-C1, GFP was removed.
RDB15038	pSpCas9-mGli3-exon3_sgRNA	Expression vector of sgRNA for targeting mouse Gli3-exon3.
RDB15037	pSpCas9-mGli3-exon2_sgRNA	Expression vector of sgRNA for targeting mouse Gli3-exon2.

(1) CDS status was determined by comparing the plasmid sequence with NCBI RefSeq mRNA.

webcatalog20240727.tab

NIA/NIH Mouse cDNA Clone

Plasmid request [in Japanese] [in English]

Catalog number	Clone name	Vector	Sequence		Status
Catalog number	Clone name	Vector	5' terminal EST, submitted in DDBJ⁽¹⁾	3' terminal EST, submitted in DDBJ⁽¹⁾	Status
MCH052099	H3130E03	pSPORT1	BG086782	BG074069

♦ Full length sequence is not available. The clone could differ from the NCBI mRNA reference sequence.
(1) Actual EST sequence either 5' or 3' terminal end. Data were submitted to the DNA Data Bank of Japan (DDBJ).
♦ NIA/NIH Mouse cDNA clone was deposited by Dr Minoru Ko (Tanaka et al., PNAS 97, 9127-9132, 2000. VanBuren V, et al. Genome Research 12, 1999-2003, 2002) at the National Institute on Aging, the National Institutes of Health, U.S.A.

NIAmouse22k_2024May11.csv

Mouse BAC clone

Clone request [in Japanese] [in English]

BAC clone search	[map around Gene ID 14634] (link to our BAC browser)

♦ Every single our BAC clones have analyzed their terminal end sequences to map on the genome. Most of them are assigned by both ends and indicated with blue line on the genome browser (they likely contain the region). Others are assigned only by a single end and indicated with red line on the genome browser (containing piece is unsure).
♦ Reasons that we did not use the other end result for mapping are: (1) Data was not suitable for BLAST analysis (short read, repeats, no data or such stuff); (2) Result of BLAST analysis is unlikely [two ends are, too far away (>0.3 Mb), distinct chromosome, same orientation, or such incomprehensible].
♦ Full length sequence is not available.

2024.09.24

Mus_musculus_gene_info230514.csv - RDB_mmu_GIxxxxxxxxx_html_240727.pl