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pCMV-3xFLAG-mGli3_WT

Expression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG. Though this clone was constructed based on the pEGFP-C1, GFP was removed.

Catalog number RDB15039
Resource name pCMV-3xFLAG-mGli3_WT
Clone info. Expression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG, see Fig3A of Makino, S. et al. Sci. Rep., 6: 39608, 2016.
Expression was confirmed by the depositor with direct sequencing and western blotting.
Vector backbone
pEFGP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Gli3 cDNA
Depositor Gondo, Yoichi | Makino, Shigeru |
 
Other clones in our bank mouse Gli3 (NCBI Gene 14634) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino,S. et al. Sci. Rep., 6: 39608, 2016).
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15039 pCMV-3xFLAG-mGli3_WT Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783.

2019.07.19

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