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pCMV-3xFLAG-mGli3_WT

Expression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG. Though this clone was constructed based on the pEGFP-C1, GFP was removed.

Catalog number RDB15039
Resource name pCMV-3xFLAG-mGli3_WT
Clone info. Expression vector of mouse Gli3, wild type, tagged with N-terminal 3xFLAG, see Fig3A of Makino, S. et al. Sci. Rep., 6: 39608, 2016.
Comment Expression was confirmed by the depositor with direct sequencing and western blotting.
Vector backbone
pEFGP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Gli3 cDNA
Depositor|Developer Gondo, Yoichi | Makino, Shigeru |
Other clones in our bank
External Database
mouse Gli3
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino,S. et al. Sci. Rep., 6: 39608, 2016).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino,S. et al. Sci. Rep., 6: 39608, 2016)。
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          RDB15039 pCMV-3xFLAG-mGli3_WT Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl