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pSpCas9-mGli3-exon2_sgRNA

Expression vector of sgRNA for targeting mouse Gli3-exon2.

Catalog number RDB15037
Resource name pSpCas9-mGli3-exon2_sgRNA
Clone info. Expression vector of sgRNA for targeting mouse Gli3-exon2, SpCAS9 and 2A-Puro, see Fig1B of Makino, S. et al. Sci. Rep., 6: 39608, 2016.
Expression was confirmed by the depositor with direct sequencing and western blotting. Recommended host, Stbl3.
Vector backbone pSpCas9(BB)-2A-Puro (PX459) (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Gli3 genomic
Depositor Gondo, Yoichi | Makino, Shigeru |
 
Other clones in our bank mouse Gli3 (NCBI Gene 14634) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino,S. et al. Sci. Rep., 6: 39608, 2016).
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (genome editing).
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15037 pSpCas9-mGli3-exon2_sgRNA Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783.

2019.07.19

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