Expression vector of mouse mutant Gli3, one-base-pair deletion of 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.
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External Database mouse Gli3 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino,S. et al. Sci. Rep., 6: 39608, 2016). |
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Ordering | Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino,S. et al. Sci. Rep., 6: 39608, 2016)。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15041 | pCMV-3xFLAG-mGli3_del97G-HA | DNA solution |
Electronic file
Original reference
original | Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15041_A7Eop1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward Region: CMV pro, 3xFLAG, insert 5' (del97G) Sequence file: RDB15041_A7Eoa.seq |
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>D04539A5_A7Eo_1_CMV-Forword_G02_20_ABI24.ab1 1 AGTCAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCACCA TGGACTACAA AGACCATGAC 121 GGTGATTATA AAGATCATGA CATCGATTAC AAGGATGACG ATGACAAGGA GGCCCAGGCC 181 CACAGCTCTA CGGCGACTGA GAGGAAGAAA GCTGAAAATT CCATTGGGAA ATGTCCCACG 241 AGAACAGATG TCAGCGAGAA GCCGTGGCCT CTAGTACCAC TTCCAATGAG GATGAAAGTC 301 CTGGACAGAT CTATCACCGA GAGAGAAGAA ACGCAATCAC TATGCAGCCT CAGAGTGTGC 361 AGGGTCTCAA CAAAATCAGT GAGGAGCCCT CGACGTCTAG TGATGAGAGG GCCTCGCTGA 421 TCAAGAAAGA GATCCATGGC TCTCTACCAC ATCTGGCGGA GCCCTCTCTC CCTTACCGTG 481 GGACTGTGTT TGCCATGGAT CCCCGGAATG GCTACATGGA GCCTCACTAC CACCCTCCTC 541 ATCTTTTCCC TGCCTTCCAT CCTCCTGTAC CAATTGATGC CAGACATCAT GAGGGCCGTT 601 ACCATTATGA TCCATCTCCT ATTCCTCCAT TACATGTGCC TTCTGCCTTA TCTAGTAGCC 661 CGACGTATCC AGACTTGCCC TTCATTAGGA TCTCCCCACA CCGTAATCCC ACTGCAGCCT 721 CAGAGTCCCC CTTCAGCCCC CCACACCCCT ACATCAACCC ATATATGGAC TACATCCGCT 781 CATTGCACAG CAGCCCATCC CTCTCCATGA TCTCTGCTGC CCGAGGGCTG AGCCCTACAG 841 ATGCTCCCCA TGCTGGAGTC AGCCCTGCGG AATACTATCA CCAGATGGCT CTGCTGACAG 901 GCCAGCGCAG CCCTTATGCA GACATCCTTC CCTCAGCTGC CACTGCTGGT GCACGGGCCA 961 TCCATATGGA GTACCCTTCA TGCCATGGAC AGCACCAGAT TTCCCAGCCC TAGCTGTCAG 1021 CTAGCCAGGC CGGAAGCGCA CACTGTCATA TCGCCACTGT CAGATCCATA GCCTTCCGAC 1081 ATTCGACCAT GATAGACATC CCCCTACTCT GGTTTAACAA TTCCCTCCAA T // |
Primer: SV40polyA-R Region: HA, insert 3' Sequence file: RDB15041_A7Eob.seq |
>D04539A5_A7Eo_1_SV40polyA-R_H02_23_ABI24.ab1 1 AAAAGTGGGA GTTTTAGCAG TAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT 61 ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC TGCAGAATTC CTATTAAGCG 121 TAATCTGGAA CATCGTATGG GTAGGCCTGC ATAACTGCAA GGAACTTGCT TTCTTCTGCA 181 AGGGAGGTCA GCAAAGAACT CATATCCCCG ATAGCCATGT TGGTGGTGCC CATGGATAGG 241 GATGGGAATG GGAGGGATGC CCGCGGAGTG GTGAGACGGG AGGAGCTGTG GGAAAGGTTC 301 TGAATAATAC TTGGGCTCAA GGCCCCTGAC ATTAGGCTGG TATGGTCCCC ATCATCTATG 361 ATGGCATCAA AATCAATCTG CACACCTTCT AGGTCATGAC TCTCAAAGCT GTCAACTGTG 421 CTTGTTACCT GGTTAGCACC TGGGGAGAGT AACTCAGAGT TTTCAGTGCC ATTCCCTTGG 481 AGCAGGCAGT TGGCATCTGA GAGGGAGAAC GAGCCAGCTT TCCTGTCTTG CTGACTGAAG 541 CCCATGGTTT GGTCATAGAA CTGACCGGAA TAATTCTGCA CGGTAGAGCA GAGCTGCTGG 601 GACTGACCTT GTGCCTCCAT TTTGATGCTG TTCACCCTGC TGCTCTGACT CATGTCACTG 661 AGCTGTCCTT GTTGCAGAGT GAGGTTGCCC CTTGGCATTG CCTGACGCCT GTTACCCCCA 721 TAGCTGGCAC AGGGCTGGTA GCCCCTGATA GCTGCCATGC TTGATGGCTG GCTGCTGTTG 781 TCCTGAGTCC CTGGTAGGCA GCTCTCTGTC CCTTGATAGA TGTTGATATG TGAGGTAGCA 841 CCAACCTGCC CTAGTACCTG TTGACCAGGG CGGCTGGGCC CCTGGTGTTG CATGCTGCCA 901 CTGAGTAGCT GATGGGCAAT GTATCCCTGT CCCATTTGAT CTTGGGTTTG CATGCCATTC 961 ACTGTGTTGC CAGCTAACTC ATTTGGTGCC ACGGGACAGG CTGAAATCTA GCAACCCCCA 1021 TTCTCTTGCA AGCTGGTGCG TTTAGCTGAC CCCTTGAAAT CCCTGTACAC AGCCATGATC 1081 TAGAGCACTG GAGTCATTGT CTGGCCTGTG GATCTGACTC GATCACTGCT GCTCAAGGGC 1141 TGATTCCATG // |
Primer: SV40pro_ori_F Region: SV40pro_ori, NeoR_KanR Sequence file: RDB15041_A7Eoc.seq |
>15041_15041_A9IbF_SV40pro_ori_F_F06_18_ABI24.ab1 1 NNNNNNNNNN NNNGCNGCCT CGNCCTCTGN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT 61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA 121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT 181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG 301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG 361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC 421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC 481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC 601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG 661 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC 721 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CANGACATAG 781 CGTTGGCTAC CCGTGATATT GCTGAAGANC TTGGCGGCGA ATGNGCTGAC CGCTTCCTCG 841 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC NNTCTATCGC CTTCTTGNNG 901 AGTNCTTCTG ANCGGGNNTC NGGGGGTTCN AAATGANNGA NNNANCGANN NNNNANNNGN 961 NNTCNNNANN TTNNNATTNN NNNGNNNNNC NTNNNNNNNA NNNNNNG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. |
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2022.05.17
GNP_filter3_RDBDEP_html_220516.pl