Resource data sheet
DNA Bank Top

pCMV-3xFLAG-mGli3_del97G-HA (#RDB15041)

Expression vector of mouse mutant Gli3, one-base-pair deletion of 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Makino, S., Sci. Rep. 6: 39608 (2016). PMID 28000783. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse mutant Gli3, one-base-pair deletion of 97G, tagged with N-terminal 3xFLAG and C-terminal HA, see Fig3A of Makino, S. et al. Sci. Rep., 6: 39608, 2016.
Comment Expression was confirmed by the depositor with direct sequencing and western blotting.
Vector backbone
pEFGP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Growth conditions
LB+Km, 37oC
Gene/insert name mouse Gli3 cDNA
Depositor|Developer Gondo, Yoichi | Makino, Shigeru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino,S. et al. Sci. Rep., 6: 39608, 2016).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino,S. et al. Sci. Rep., 6: 39608, 2016)。

Catalog # Resource name Shipping form Fee
RDB15041 pCMV-3xFLAG-mGli3_del97G-HA DNA solution

How to cite this biological resource

Materials & Methods section:

The pCMV-3xFLAG-mGli3_del97G-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15041).

Reference section:

Makino, S., Fukumura, R., Gondo ,Y., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Makino, S., Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci. Rep. 6: 39608 (2016). PMID 28000783. [PubMed] [Article] [RRC of NBRP]