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Tomoji Mashimo's Resource

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Catalog #Name of cloneRunning titlePublication
RDB18214pBS-U6-crRNA-emptyExpression vector of crRNA, U6 promoter for genome editing tool.Morisaka, H., CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nat. Commun. 10 (1): 5302 (2019). PMID 31811138. (original). [link to RRC of NBRP]
RDB18213pPB-CAG-hCas3Expression vector of Cas3 with bpNLS, CAG promoter for genome editing tool.Morisaka, H., CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nat. Commun. 10 (1): 5302 (2019). PMID 31811138. (original). [link to RRC of NBRP]
RDB18212pCAG-All-in-one-hCascadeExpression vector of CRISPR-associated complex for antiviral defense for genome editing tool.Morisaka, H., CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nat. Commun. 10 (1): 5302 (2019). PMID 31811138. (original). [link to RRC of NBRP]
RDB13130T7-NLS hCas9-pACas9-poly(A) expressing improved plasmid.Yoshimi, K., ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat. Commun. 7: 10431 (2016). PMID 26786405. (original). [link to RRC of NBRP]
RDB13015pT7-gRNA:kit^h -2-2 DR274Plasmid clone for in vitro transcription of sgRNA targeting rat Kit gene by Cas9.Yoshimi, K., Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat. Commun. 5:4240 (2014). PMID 24967838. (original). [link to RRC of NBRP]
RDB13014pT7-gRNA:kit^h -2-1 DR274Plasmid clone for in vitro transcription of sgRNA targeting rat Kit gene by Cas9.Yoshimi, K., Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat. Commun. 5:4240 (2014). PMID 24967838. (original). [link to RRC of NBRP]
RDB13013pT7-gRNA:kit^h -1 DR274Plasmid clone for in vitro transcription of sgRNA targeting rat Kit gene by Cas9.Yoshimi, K., Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat. Commun. 5:4240 (2014). PMID 24967838. (original). [link to RRC of NBRP]
RDB13012pT7-gRNA:Asip^a DR274Plasmid clone for in vitro transcription of sgRNA targeting rat Asip gene by Cas9.Yoshimi, K., Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat. Commun. 5:4240 (2014). PMID 24967838. (original). [link to RRC of NBRP]
RDB13011pT7-gRNA:Tyr^c DR274 (wild)Plasmid clone for in vitro transcription of sgRNA targeting rat Tyr^c (wild) gene by Cas9.Yoshimi, K., Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat. Commun. 5:4240 (2014). PMID 24967838. (original). [link to RRC of NBRP]
RDB13010pT7-gRNA:Tyr^c DR274 (albino)Plasmid clone for in vitro transcription of sgRNA targeting rat Tyr^c (albino) gene by Cas9.Yoshimi, K., Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat. Commun. 5:4240 (2014). PMID 24967838. (original). [link to RRC of NBRP]

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A bicistronic cell cycle reporter, Fucci2a (Sep 17, 2024)
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High intensity forms of luciferase and luminescent proteins from various organisms (BRC RESOURCE NEWS) (Apr 28, 2023)
Plasmid of Cas9 expression/mRNA production, evaluation of the genome edit efficiency, and Knock-in donors and tags
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2025.03.28

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