Resource data sheet
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T7-NLS hCas9-pA (#RDB13130)

Cas9-poly(A) expressing improved plasmid.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Yoshimi, K., Nat. Commun. 7: 10431 (2016). PMID 26786405. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Cas9 expressing plasmid. Induction with CMV or T7 promoter. Applicable for both direct transfection into mammalian cells and T7 in-vitro translation.
Comment Digest with Nhe I for linearization prior to in vitro transcription followed by capping and polyadenylation.
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Growth conditions 37oC, LB + Amp.
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA
Depositor|Developer Mashimo, Tomoji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshimi, K. et al. Nat. Commun. 7: 10431, 2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with The University of Tokyo.
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yoshimi, K. et al. Nat. Commun. 7: 10431, 2016)。非営利機関が非営利目的の教育・研究用に用いる場合以外は、東京大学と別途MTAを締結すること。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee
RDB13130 T7-NLS hCas9-pA DNA solution

How to cite this biological resource

Materials & Methods section:

The T7-NLS hCas9-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13130).

Reference section:

Yoshimi, K., Kunihiro, Y., Kaneko, T., Nagahora, H., Voigt, B., Mashimo, T., ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat. Commun. 7: 10431 (2016). PMID 26786405. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yoshimi, K., ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat. Commun. 7: 10431 (2016). PMID 26786405. [PubMed] [Article] [RRC of NBRP]
reference Hashikawa, Y., Generation of Knockout Rabbits With X-linked Severe Combined Immunodeficiency (X-SCID) Using CRISPR/Cas9. Sci. Rep. 10 (1): 9957 (2020). [Article]
user_report Mizuno-Iijima, S., Efficient production of large deletion and gene fragment knock-in mice mediated by genome editing with Cas9-mouse Cdt1 in mouse zygotes. Methods 191: 23-31 (2021). PMID 32334080. [PubMed] [Article] [RRC of NBRP]
user_report Suzuki, K., Deletion of B-cell translocation gene 2 (BTG2) alters the responses of glial cells in white matter to chronic cerebral hypoperfusion. J. Neuroinflammation 18 (1): 86 (2021). PMID 33812385. [PubMed] [Article] [RRC of NBRP]
user_report Osawa, Y., EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Elife 10: e59759 (2021). PMID 33973520. [PubMed] [Article] [RRC of NBRP]
user_report Matsumura, K., Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes. Nat. Commun. 11 (1): 859 (2020). PMID 32103003. [PubMed] [Article] [RRC of NBRP]
user_report Ayabe, S., Off- and on-target effects of genome editing in mouse embryos. J. Reprod. Dev. 65 (1): 1-5 (2019). PMID 30518723. [PubMed] [Article] [RRC of NBRP]
user_report Miyasaka, Y., CLICK: one-step generation of conditional knockout mice. BMC Genomics 19 (1): 318 (2018). PMID 29720086. [PubMed] [Article] [RRC of NBRP]