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pPB-CAG-hCas3 (#RDB18213)

Expression vector of Cas3 with bpNLS, CAG promoter for genome editing tool.


Drawn by SnapGene® software
Sequence (full) RDB18213hts01.seq
Publication Morisaka, H., Nat. Commun. 10 (1): 5302 (2019). [link to RRC of NBRP]
Test sheet RDB18213C0p1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of Cas3 with bpNLS, CAG promoter for genome editing tool, TypeI-E CRISPR/Cas3 system in mammalian cells. A components for genome editing in mammalian cells. Used with pCAG-All-in-one-hCascade (RDB18212) and pBS-U6-crRNA-empty (RDB18214) containing a target sequence.
Vector backbone Plasmid
Gene/insert name E. coli Cas3.
Depositor|Developer Mashimo, Tomoji |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morisaka, H. et al. Nat. Commun.10(1), 5302, 2019)
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RDB18213 pPB-CAG-hCas3 DNA solution

How to cite this biological resource

Materials & Methods section:

The pPB-CAG-hCas3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18213).

Reference section:

Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., Mashimo, T., CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nat. Commun. 10 (1): 5302 (2019). PMID 31811138. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Morisaka, H., CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nat. Commun. 10 (1): 5302 (2019). PMID 31811138. [link to RRC of NBRP]