Resource data sheet
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pT7-gRNA:kit^h -2-2 DR274 (#RDB13015)

Plasmid clone for in vitro transcription of sgRNA targeting rat Kit gene by Cas9.

Alternative name Kit2-2
Clone info. Plasmid clone for in vitro transcription of sgRNA targeting rat Kit gene by Cas9.
Comment Nucleotide sequence: 5' TAGGGTCAAGATGTCATCTTACGG 3'.
Vector backbone DR274 (Plasmid)
Size of vector backbone 2.2 kb
Gene/insert name Rat kit genomic DNA
Depositor|Developer Mashimo, Tomoji |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nat Commun. 5:4240, 2014).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat Commun. 5:4240, 2014)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13015 pT7-gRNA:kit^h -2-2 DR274 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pT7-gRNA:kit^h -2-2 DR274 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13015).

Reference section:

Yoshimi, K., Kaneko, T., Voigt, B., Mashimo, T., Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat. Commun. 5:4240 (2014). PMID 24967838. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yoshimi, K., Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat. Commun. 5:4240 (2014). PMID 24967838. [link to RRC of NBRP]