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Noriaki Shimizu's Resource

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Catalog #Name of cloneRunning titlePublication
RDB18711pD8Plasmid clone of 2,347 bp Chinese hamster D8 sequence, which was isolated from DHFR replication origin as a minimum sequence required for gene amplification.Ogaki, Y., Repeat induces not only gene silencing, but also gene activation in mammalian cells. PLoS One 15 (6): e0235127 (2020). PMID 32579599. [PubMed] [Article] [RRC of NBRP] (original)
RDB18710pC12Plasmid clone of 798 bp human C12 sequence, which was isolated from MYC replication origin as a minimum sequence required for gene amplification.Ogaki, Y., Repeat induces not only gene silencing, but also gene activation in mammalian cells. PLoS One 15 (6): e0235127 (2020). PMID 32579599. [PubMed] [Article] [RRC of NBRP] (original)
RDB18709pG5Plasmid clone of 972 bp human G5 sequence, which was isolated from beta-globin replication origin as a minimum sequence required for gene amplification.Ogaki, Y., Repeat induces not only gene silencing, but also gene activation in mammalian cells. PLoS One 15 (6): e0235127 (2020). PMID 32579599. [PubMed] [Article] [RRC of NBRP] (original)
RDB18708pB-3-31Plasmid clone of 3,271 bp human genomic sequence, B-3-31, which is known to enhance the expression of co-transfected expression vectors.Ogaki, Y., Repeat induces not only gene silencing, but also gene activation in mammalian cells. PLoS One 15 (6): e0235127 (2020). PMID 32579599. [PubMed] [Article] [RRC of NBRP] (original)
RDB18707pdeltaBM.AR1.d2EGFPA clone for the IR/MAR gene amplification method. This clone is used for monitoring the gene amplification by observation of GFP fluorescence.Ogaki, Y., Repeat induces not only gene silencing, but also gene activation in mammalian cells. PLoS One 15 (6): e0235127 (2020). PMID 32579599. [PubMed] [Article] [RRC of NBRP] (original)
RDB18706pdeltaBM.AR1.MCSA clone for the IR/MAR gene amplification method. By cloning an expression unit of interested gene into the MCS, amplified gene expression can be obtained.Araki, Y., Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method. PLoS One, 7 (7): e41787 (2012). PMID 22844523. [PubMed] [Article] [RRC of NBRP] (original)
RDB18705pdeltaBN.AR1A clone for the IR/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells.Shimizu, N., Amplification of plasmids containing a mammalian replication initiation region is mediated by controllable conflict between replication and transcription. Cancer Res. 63 (17): 5281-5290 (2003). PMID 14500359. [PubMed] [RRC of NBRP] (original)
RDB18704pSFVdhfrA clone for the IR/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells. Shimizu, N., Plasmids with a mammalian replication origin and a matrix attachment region initiate the event similar to gene amplification. Cancer Res. 61 (19): 6987-6990 (2001). PMID 11585721. [PubMed] [RRC of NBRP] (original)
RDB01198ETR103Human Egr- 1 cDNA, TPA early response geneShimizu, N., A gene coding for a zinc finger protein is induced during 12-O-tetradecanoylphorbol-13-acetate-stimulated HL-60 cell differentiation. J. Biochem., 111, 272-277 (1992). PMID 1569051. [PubMed] [Article] (original)
RDB01197ETR101Human Chx I (mouse), pip92 (mouse) like cDNA, TPA early response geneShimizu, N., Expression of a novel immediate early gene during 12-O-tetradecanoylphorbol-13-acetate-induced macrophagic differentiation of HL-60 cells. J. Biol. Chem., 266, 12157-12161 (1991). PMID 2061303. [PubMed] (original)

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2025.05.21

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