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pdeltaBN.AR1 (#RDB18705)

A clone for the IR/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Shimizu, N., Cancer Res. 63 (17): 5281-5290 (2003). PMID 14500359. [PubMed] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. A clone for the IR/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells. This clone contains mammalian origin of replication from the Ori beta region downstream of the Chinese hamster DHFR gene, AR1 sequence form mouse Igk intron (MAR) and blasticidine resistance gene. Please refer Fig.5 of Shimizu, N. et al., Cancer Res., 63 (17): 5281-5290, 2003 and Fig.1 of Araki, Y. et al., PLoS ONE 7 (7): e41787, 2012.
Comment Blasticidine resistance
Vector backbone pBR322 (plasmid)
Size of vector backbone 4.4 kb
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name hamster Dhfr genomic
Depositor|Developer Shimizu, Noriaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shimizu, N. et al., Cancer Res., 63 (17): 5281-5290, 2003). The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution.
提供案内 (日本国内) [open/close]

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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shimizu, N. et al., Cancer Res., 63 (17): 5281-5290, 2003)。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

Catalog # Resource name Shipping form Fee
RDB18705 pdeltaBN.AR1 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pdeltaBN.AR1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18705).

Reference section:

Shimizu, N., Hashizume, T., Shingaki, K., Kawamoto, J.K., Amplification of plasmids containing a mammalian replication initiation region is mediated by controllable conflict between replication and transcription. Cancer Res. 63 (17): 5281-5290 (2003). PMID 14500359. [PubMed] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shimizu, N., Amplification of plasmids containing a mammalian replication initiation region is mediated by controllable conflict between replication and transcription. Cancer Res. 63 (17): 5281-5290 (2003). PMID 14500359. [PubMed] [RRC of NBRP]