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pG5 (#RDB18709)

Plasmid clone of 972 bp human G5 sequence, which was isolated from beta-globin replication origin as a minimum sequence required for gene amplification.


Drawn by SnapGene® software
Sequence (full) RDB18709hts02.seq
Publication Ogaki, Y., PLoS One 15 (6): e0235127 (2020). [link to RRC of NBRP]
Publication Ohsaki, K., PLoS One 12 (4): e0175585 (2017). [link to RRC of NBRP]
Publication Okada, N., PLoS One 8 (10): e77350 (2013). [link to RRC of NBRP]
Test sheet RDB18709_B1Bhp1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Plasmid clone of 972 bp human G5 sequence, which was isolated from beta-globin replication origin as a minimum sequence required for gene amplification. By PCR-amplifying the G5 sequence and incorporating it into an expression vector or by the transfection with an expression vector along with tandem repeat sequence made by ligation of the PCR-amplified G5 sequence, cells with high expression of the gene of interest can be obtained. Please refer to Fig.4 of Okada, N. et al. PLoS One, 8 (10): e77350, 2013.
Comment Blasticidine resistance
Vector backbone pBR322 (plasmid)
Size of vector backbone 4.4 kb
Selectable markers Ampicillin
Gene/insert name human HBB genomic
Depositor|Developer Shimizu, Noriaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Okada, N. et al. PLoS One, 8 (10): e77350, 2013); (Ogaki, Y., et al. PLoS One, 15 (6): e0235127, 2020); (Ohsaki, K., et al., PLoS One, 12 (4): e0175585, 2017). The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Okada, N. et al. PLoS One, 8 (10): e77350, 2013); (Ogaki, Y., et al. PLoS One, 15 (6): e0235127, 2020); (Ohsaki, K., et al., PLoS One, 12 (4): e0175585, 2017) 。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18709 pG5 DNA solution

How to cite this biological resource

Materials & Methods section:

The pG5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18709).

Reference section:

Ogaki, Y., Fukuma, M., Shimizu, N., Repeat induces not only gene silencing, but also gene activation in mammalian cells. PLoS One 15 (6): e0235127 (2020). PMID 32579599. [link to RRC of NBRP]
Ohsaki, K., Ohgaki, Y., Shimizu, N., Amplification of a transgene within a long array of replication origins favors higher gene expression in animal cells. PLoS One 12 (4): e0175585 (2017). PMID 28403180. [link to RRC of NBRP]
Okada, N., Shimizu, N., Dissection of the beta-globin replication-initiation region reveals specific requirements for replicator elements during gene amplification. PLoS One 8 (10): e77350 (2013). PMID 24124615. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ogaki, Y., Repeat induces not only gene silencing, but also gene activation in mammalian cells. PLoS One 15 (6): e0235127 (2020). PMID 32579599. [link to RRC of NBRP]
original Ohsaki, K., Amplification of a transgene within a long array of replication origins favors higher gene expression in animal cells. PLoS One 12 (4): e0175585 (2017). PMID 28403180. [link to RRC of NBRP]
original Okada, N., Dissection of the beta-globin replication-initiation region reveals specific requirements for replicator elements during gene amplification. PLoS One 8 (10): e77350 (2013). PMID 24124615. [link to RRC of NBRP]