A clone for the IR/MAR gene amplification method. This clone is used for monitoring the gene amplification by observation of GFP fluorescence.
Drawn by SnapGene® software |
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Clone info. | A clone for the IR/MAR gene amplification method. This clone is used for monitoring the gene amplification by observation of GFP fluorescence. This clone contains a destabilized EGFP expression unit, mammalian origin of replication from the Ori beta region downstream of the Chinese hamster DHFR gene, AR1 sequence form mouse Igk intron (MAR) and blasticidine resistance gene. Please refer Fig.1 of Harada, S. et al., J. Biol. Chem., 284 (36): 24320-24327, 2009, Fig.1 of Ohsaki, K. et al, PLoS ONE 12 (4): e0175585m 2017 and Ogaki, Y. et al., PLoS ONE 15 (6): e0235127, 2020. |
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Comment | Blasticidine resistance |
Vector backbone | pBR322 (plasmid) |
Size of vector backbone | 4.4 kb |
Selectable markers | Ampicillin |
Gene/insert name | hamster Dhfr genomic |
Depositor|Developer | Shimizu, Noriaki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Harada, S. et al., J. Biol. Chem., 284 (36): 24320-24327, 2009); (Ogaki, Y., et al. PLoS One, 15 (6): e0235127, 2020). The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Harada, S. et al., J. Biol. Chem., 284 (36): 24320-24327, 2009); (Ogaki, Y., et al. PLoS One, 15 (6): e0235127, 2020)。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18707 | pdeltaBM.AR1.d2EGFP | DNA solution |
Materials & Methods section:
The pdeltaBM.AR1.d2EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18707). |
Reference section:
Ogaki, Y., Fukuma, M., Shimizu, N., Repeat induces not only gene silencing, but also gene activation in mammalian cells. PLoS One 15 (6): e0235127 (2020). PMID 32579599. [link to RRC of NBRP] |
Harada, S., Uchida, M., Shimizu, N., Episomal high copy number maintenance of hairpin-capped DNA bearing a replication initiation region in human cells. J. Biol. Chem. 284 (36): 24320-24327 (2009). PMID 19617622. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Ogaki, Y., Repeat induces not only gene silencing, but also gene activation in mammalian cells. PLoS One 15 (6): e0235127 (2020). PMID 32579599. [link to RRC of NBRP] |
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original | Harada, S., Episomal high copy number maintenance of hairpin-capped DNA bearing a replication initiation region in human cells. J. Biol. Chem. 284 (36): 24320-24327 (2009). PMID 19617622. [link to RRC of NBRP] |
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