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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pETDuet-2B4-2B2_dL314Q (#RDB18642)

Bacterial expression vector of human eIF2Bdelta mutant (L314Q) and human eIF2Bbeta.

Clone info. Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit delta (eIF2Bdelta) mutant (L314Q) and human eukaryotic translation initiation factor 2B subunit beta (eIF2Bbeta) cloned into the pETDuet-1.
Comment Expression was confirmed by the depositor.
Vector backbone pETDuet-1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human EIF2B2 cDNA human EIF2B4 cDNA
Depositor|Developer Ito, Takuhiro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。

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RDB18642 pETDuet-2B4-2B2_dL314Q DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pETDuet-2B4-2B2_dL314Q was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18642).

Reference section:

Zyryanova, A.F., Kashiwagi, K., Rato, C., Harding, H.P., Crespillo-Casado, A., Perera, L.A., Sakamoto, A., Nishimoto, M., Yonemochi, M., Shirouzu, M., Ito, T., Ron, D., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18642_B0Lbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R (Pr0065)
Region: lacO, RBS, insert 5' (2B4 5')
Sequence file: RDB18642_B0Lba.seq check
>D07570A4_B0Lb_2_pBR322_BamHI_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NANNANTNNC TATANNNNAA TTGTGAGCGG ATAACAATTC
   61 CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACCATGGGC AGCAGCAGCC
  121 AGGATCCGAA TTCGAGCTCG GCGCGCCTGC AGGTCGACGC TGCTGTGGCC GTGGCTGTTC
  181 GCGAGGACTC GGGATCCGGG ATGAAGGCGG AGCTTCCCCC CGGGCCTGGG GCAGTGGGGA
  241 GGGAAATGAC CAAAGAAGAA AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA
  301 AACGGAAGGA AGAAAAGGGG GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT
  361 GTCAAGTAGG CCCAACCAGA GAACTGCCAG AATCGGGCAT TCAGTTGGGC ACTCCTCGGG
  421 AGAAAGTTCC AGCTGGTCGG AGTAAGGCCG AACTTCGGGC TGAGCGTCGA GCCAAGCAGG
  481 AGGCCGAGCG GGCCCTGAAA CAGGCAAGAA AAGGGGAACA AGGAGGACCA CCTCCTAAGG
  541 CCAGCCCCAG CACAGCTGGA GAAACCCCCT CAGGAGTGAA GCGTCTCCCT GAGTACCCTC
  601 AGGTTGATGA CCTACTTCTG AGAAGGCTTG TTAAAAAACC AGAGCGTCAA CAGGTTCCTA
  661 CACGAAAGGA TTATGGATCC AAAGTCAGTC TCTTCTCTCA CCTACCCCAG TACAGCAGAC
  721 AAAACTCTCT GACCCAGTTT ATGAGCATCC CATCCTCTGT GATCCACCCA GCCATGGTGC
  781 GACTCGGCCT GCAGTACTCC CAGGGCCTGG TCAGTGGCTC CNATGCCCGG NGTATTGCCC
  841 TGCTTCGTGN CTTGCAGCAG GTGATTCANG ATTACACNAC ACCGCCTAAT GAAGAANTCT
  901 CCAGGGATCT AGTGAATAAA CTAAAACCCT ACNTGANCTT NCTGANTCAG NGNCGTCCCC
  961 NGNCAGCGAG CATGNNCAAC GCCNTNNNGT TNCTTNNCNN NGAAATCANC AGNGNGGGCA
1021 NTTNCNNGNN NNNANNANNN NNANNCNGAN NTTNNNNNNN NNN
//
Primer: T7 terminator (Pr0155)
Region: insert 3' (2B2 3')
Sequence file: RDB18642_B0Lbb.seq check
>D07570A4_B0Lb_2_T7 terminator_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCN NNNANNNGAN TCNNTGTGCT GGCGTTCAAA TTTCGCAGCA
   61 GCGGTTTCTT TACCAGACTC GAGGGTACCG ACGTCTCATA AAACATGATC ATCAGGATGG
  121 TAGAGTTCAC TCATCAGGCG GTAGATGTAG GAAGGTGCAT TCCCACCAAT GTTGGAGATA
  181 AAGAGGGTAA TGAGCTCTGG GGGAACGTAG TCAAACACAG GGCAATGCAC GCTGACCTTC
  241 TCCAGAATGT CCCCTTCTGT GAATGGCAGG ACTTCTTCAG GAGCCACAAA CTTATGAAAT
  301 GAGTCTTCTT CATTGGGGAA CTGTGGAGAA AGTTTGAACA TAGGTGCACA GACGATGAGT
  361 GGGGTGGAAT GGTGTTTTGC TGCCAGTGCC AGAGTGTGAG TTCCTGTCAC AGCTCTCAGG
  421 GCCCCATTGG CCAGGATGGT CTTCGTGCCA ATGATCACCT TGTTGACTCT TGACATAACG
  481 GCAAAAATGG CAGCATCAGT CATGACAGTT GTCTCAATAC CTGCTTTGGA CAAATTCACA
  541 GCCATTTCAT GACCCTGGCA GAAAGGAGCA CACTCTGCTA CAATGACATG GAATTTCCTC
  601 TTTCGGGCAG CCTCTTTGAG GAAGGCCTCT ACTGTTCGGG AGAAGCCAAT GGTCATGATC
  661 ACCTCATTGG AGTGAATGTG CTCCAGAGCC TGGGCTGCAA TGTTCTCCAT TGTCCCTTCC
  721 AGCTCCACTA GCAGCTCATT AATCGCCTCA ATGATGTTGG ACTGGAGTTG GGCATAATGG
  781 AAGCTGAAAT CCTCGTTTAN GNCTCCGGAT GTCAACAGTT TGTGCAGGGA CTCCTGCTGN
  841 TCACTCTCGT CGCTGCGTCC ATGGANTCTG CCATACTCCT CCCGGANAAT CTTGANCANT
  901 CTCCGCACCA TGTTGCCCAC NGNGGTCTCN GANGNNTGAN CGGCCGTCAT CCTCCTGNCN
  961 NCTCTGNNGA TCAGCTCCNT CANCTCCCCC GCGTTGNTCC NGCNGNGGNC CGNGNNGNNC
1021 TGNNNCANNN NCCCNANGGN CNCCNNNNNN NTTTNNNNNN NNNN
//
Primer: hEIF2B-M_F1 (Pr0935)
Region: insert mid (2B4 L314Q)
Sequence file: RDB18642_B0Lbc.seq check
>D07570A4_B0Lb_2_hEIF2B-M_F1_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCC CTCNGGANTG AAGCGTCTCC CTGAGTACCC
   61 TCAGGTTGAT GACCTACTTC TGAGAAGGCT TGTTAAAAAA CCAGAGCGTC AACAGGTTCC
  121 TACACGAAAG GATTATGGAT CCAAAGTCAG TCTCTTCTCT CACCTACCCC AGTACAGCAG
  181 ACAAAACTCT CTGACCCAGT TTATGAGCAT CCCATCCTCT GTGATCCACC CAGCCATGGT
  241 GCGACTCGGC CTGCAGTACT CCCAGGGCCT GGTCAGTGGC TCCAATGCCC GGTGTATTGC
  301 CCTGCTTCGT GCCTTGCAGC AGGTGATTCA GGATTACACA ACACCGCCTA ATGAAGAACT
  361 CTCCAGGGAT CTAGTGAATA AACTAAAACC CTACATGAGC TTCCTGACTC AGTGCCGTCC
  421 CCTGTCAGCG AGCATGCACA ACGCCATCAA GTTCCTTAAC AAGGAAATCA CCAGTGTGGG
  481 CAGTTCCAAG CGGGAAGAGG AGGCCAAGTC AGAACTTCGA GCAGCCATTG ATCGGTATGT
  541 GCAAGAGAAG ATTGTGCAAG CAGCTCAGGC AATTTCACGC TTTGCTTACC AGAAGATCAG
  601 TAATGGAGAT GTGATCCTGG TATATGGATG CTCATCTCTG GTATCACGAA TTCTTCAGGA
  661 GGCTTGGACA GAGGGCCGGC GGTTTCGGGT GGTAGTGGTG GACAGCCGGC CATGGCTGGA
  721 AGGAAGGCAC ACACTACGTT CTCTAGTCCA TGCTGGTGTC CCAGCCTCCT ACCTGCTGAN
  781 TCCTGCAGCC TCCTATGTGC TCCCAGANGT TTCCNAGGTG CTATTGGGAG CTCATGCACT
  841 CTTGGNCAAC GGGTCTGTGA TGTCACGGGN ANGGACAGCA CAGTTAGCCC TGGTGGCTCG
  901 AGCCCATAAT GNACCAGNGC TGGNTTGCTG TGAAACATAC NAGTTCTGTG ANCGTGNGCN
  961 NACTGNNGCC TTTGNCTCTA NTGANCTANN NGNNCNTGAN GATCTGNNNN GTAANCGGGG
1021 ANAANNTGNN GCNCTGGCTN NNNNGNNNAN NNNNNNNNCC CNANNNNNNN NNN
//
Primer: hEIF2Bb_R (Pr0902)
Region: insert mid (2B2 5', RBS, lacO, T7pro, 2B4 3')
Sequence file: RDB18642_B0Lbd.seq check
>D07570A4_B0Lb_2_hEIF2Bb_R_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCGNNCGNC NNCCTCCTGC CCTCTCTGCG GNTCAGCTCC
   61 ATCAGCTCCC CCGCGTTGCT CCNGCGGTGG TCCGTGATGA TCTGGCGCAG CAACCCTAGG
  121 GTCTCCCGAG CCATTTCCTC GGAGCTGCGC GGCCCACCAC CCCGCTTCAG GGTCTCCACG
  181 AAGCTCTCGA TCCTCTCTGA CAACTCCGAG CCCTTCGCTG CGGATCCCGG CATATGTATA
  241 TCTCCTTCTT ATACTTAACT AATATACTAA GATGGGGAAT TGTTATCCGC TCACAATTCC
  301 CCTATAGTGA GTCGTATTAA TTTCGATTAT GCGGCCGTGT ACAATACGAT TACTTTCTGT
  361 TCGACTTAAG CATTATGCGG CCGCTCACTG GTCACTGCTC TTGACTCGTA GAACAACAGG
  421 TACAGAACTG CAAGGGATCA TCCCCAGCTC CGTGATCACC AGATCCACAA GCTCTGGGGG
  481 AGTCACATCA TAGACTAGAT TCAACAACCG TAGGGATGCG TGGTTCTGCC AGTTAGCCAG
  541 CGCAACATGT TCTCCCCGCT TACATTGCAG ATCATCAGGG TCATCTAGCT CATTAGAGAC
  601 AAAGGCATCA GTCTGCACAC GCTCACAGAA CTTGTATGTT TCACAGCAAA CCAGCACTGG
  661 TACATTATGG GCTCGAGCCA CCAGGGCTAA CTGTGCTGTC CCTACCCGTG ACATCACAGA
  721 CCCGTTGGCC AAGAGTGCAT GAGCTCCCAA TAGCACCTTG GAAACCTCTG GGAGCACATA
  781 NGANGCTGCA NGAATCANCA GGTANGANGC TGGGACACCA GCATGGACTA GAGAACGTAG
  841 TGTGTGCCTT CCTTCCAGCC ATGGCCGGCT GTCCACCACT ACCACCCGAA NNCGCCGGCC
  901 NNCTGTCNAA NCCTCCTGAA NANNTCNNGA NANCAGANNT GANCATCCAT ATACCNNNAT
  961 CACATCTNCN TTANTGATCN NCTGGNANNC AAAGCNNGNA NNTGNNNGAN CTGNNNGNAC
1021 NANCTTCNNN NGNNNNTANN NATNNNNGNN NGNNCNNNNN NNNNN
//
Primer: pGL4pA_R (Pr0664)
Region: lacI
Sequence file: RDB18642_B0Lbe.seq check
>D07570A4_B0Lb_2_pGL4pA_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCNGNNGNC TAATGAGTGA GCTAACTTAC NTTAATTGCG
   61 TTGCGCTCAC TGCCCGCTTT CNNGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC
  121 GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC
  181 CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG CCCTGAGAGA GTTGCAGCAA
  241 GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTAACGGCGG
  301 GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATGT CCGCACCAAC
  361 GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC GCCATCTGAT CGTTGGCAAC
  421 CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC ATGGTTTGTT GAAAACCGGA
  481 CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA ATTTGATTGC GAGTGAGANA
  541 TTTATGCCAG CCAGCCAGAN GCAGACGCGC CGAGACAGAA CTTAATGGGN CCGCTAACAG
  601 CGCGANTTGC TGGTGACCCN ATGCGANCAG ATGCTCCACG CCCAGTCGCG NANCGTCTTC
  661 NTGGGANAAA ATANTACTGN TGANGGGTGN CTGGNCNNAN ANATCNANAA ATAACGNCNG
  721 NNANNTTANN GNNNNANCTT NCNCANCNNT GNNATNCTNG NCNTNCNNCN NANANNNANN
781 GNTNNNCCNN NNGANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Zyryanova, A.F., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP]