Bacterial expression vector of human eIF2Bdelta mutant (L314Q) and human eIF2Bbeta.
Clone info. | Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit delta (eIF2Bdelta) mutant (L314Q) and human eukaryotic translation initiation factor 2B subunit beta (eIF2Bbeta) cloned into the pETDuet-1. |
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Comment | Expression was confirmed by the depositor. |
Vector backbone | pETDuet-1 (plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Ampicillin |
Growth conditions | LB+Ampicillin, 37oC |
Gene/insert name | human EIF2B2 cDNA human EIF2B4 cDNA |
Depositor|Developer | Ito, Takuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18642 | pETDuet-2B4-2B2_dL314Q | DNA solution |
Materials & Methods section:
The pETDuet-2B4-2B2_dL314Q was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18642). |
Reference section:
Zyryanova, A.F., Kashiwagi, K., Rato, C., Harding, H.P., Crespillo-Casado, A., Perera, L.A., Sakamoto, A., Nishimoto, M., Yonemochi, M., Shirouzu, M., Ito, T., Ron, D., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18642_B0Lbp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pBR322_BamHI_R (Pr0065) Region: lacO, RBS, insert 5' (2B4 5') Sequence file: RDB18642_B0Lba.seq ![]() |
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>D07570A4_B0Lb_2_pBR322_BamHI_R_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NANNANTNNC TATANNNNAA TTGTGAGCGG ATAACAATTC 61 CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACCATGGGC AGCAGCAGCC 121 AGGATCCGAA TTCGAGCTCG GCGCGCCTGC AGGTCGACGC TGCTGTGGCC GTGGCTGTTC 181 GCGAGGACTC GGGATCCGGG ATGAAGGCGG AGCTTCCCCC CGGGCCTGGG GCAGTGGGGA 241 GGGAAATGAC CAAAGAAGAA AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA 301 AACGGAAGGA AGAAAAGGGG GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT 361 GTCAAGTAGG CCCAACCAGA GAACTGCCAG AATCGGGCAT TCAGTTGGGC ACTCCTCGGG 421 AGAAAGTTCC AGCTGGTCGG AGTAAGGCCG AACTTCGGGC TGAGCGTCGA GCCAAGCAGG 481 AGGCCGAGCG GGCCCTGAAA CAGGCAAGAA AAGGGGAACA AGGAGGACCA CCTCCTAAGG 541 CCAGCCCCAG CACAGCTGGA GAAACCCCCT CAGGAGTGAA GCGTCTCCCT GAGTACCCTC 601 AGGTTGATGA CCTACTTCTG AGAAGGCTTG TTAAAAAACC AGAGCGTCAA CAGGTTCCTA 661 CACGAAAGGA TTATGGATCC AAAGTCAGTC TCTTCTCTCA CCTACCCCAG TACAGCAGAC 721 AAAACTCTCT GACCCAGTTT ATGAGCATCC CATCCTCTGT GATCCACCCA GCCATGGTGC 781 GACTCGGCCT GCAGTACTCC CAGGGCCTGG TCAGTGGCTC CNATGCCCGG NGTATTGCCC 841 TGCTTCGTGN CTTGCAGCAG GTGATTCANG ATTACACNAC ACCGCCTAAT GAAGAANTCT 901 CCAGGGATCT AGTGAATAAA CTAAAACCCT ACNTGANCTT NCTGANTCAG NGNCGTCCCC 961 NGNCAGCGAG CATGNNCAAC GCCNTNNNGT TNCTTNNCNN NGAAATCANC AGNGNGGGCA 1021 NTTNCNNGNN NNNANNANNN NNANNCNGAN NTTNNNNNNN NNN // |
Primer: T7 terminator (Pr0155) Region: insert 3' (2B2 3') Sequence file: RDB18642_B0Lbb.seq ![]() |
>D07570A4_B0Lb_2_T7 terminator_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGCN NNNANNNGAN TCNNTGTGCT GGCGTTCAAA TTTCGCAGCA 61 GCGGTTTCTT TACCAGACTC GAGGGTACCG ACGTCTCATA AAACATGATC ATCAGGATGG 121 TAGAGTTCAC TCATCAGGCG GTAGATGTAG GAAGGTGCAT TCCCACCAAT GTTGGAGATA 181 AAGAGGGTAA TGAGCTCTGG GGGAACGTAG TCAAACACAG GGCAATGCAC GCTGACCTTC 241 TCCAGAATGT CCCCTTCTGT GAATGGCAGG ACTTCTTCAG GAGCCACAAA CTTATGAAAT 301 GAGTCTTCTT CATTGGGGAA CTGTGGAGAA AGTTTGAACA TAGGTGCACA GACGATGAGT 361 GGGGTGGAAT GGTGTTTTGC TGCCAGTGCC AGAGTGTGAG TTCCTGTCAC AGCTCTCAGG 421 GCCCCATTGG CCAGGATGGT CTTCGTGCCA ATGATCACCT TGTTGACTCT TGACATAACG 481 GCAAAAATGG CAGCATCAGT CATGACAGTT GTCTCAATAC CTGCTTTGGA CAAATTCACA 541 GCCATTTCAT GACCCTGGCA GAAAGGAGCA CACTCTGCTA CAATGACATG GAATTTCCTC 601 TTTCGGGCAG CCTCTTTGAG GAAGGCCTCT ACTGTTCGGG AGAAGCCAAT GGTCATGATC 661 ACCTCATTGG AGTGAATGTG CTCCAGAGCC TGGGCTGCAA TGTTCTCCAT TGTCCCTTCC 721 AGCTCCACTA GCAGCTCATT AATCGCCTCA ATGATGTTGG ACTGGAGTTG GGCATAATGG 781 AAGCTGAAAT CCTCGTTTAN GNCTCCGGAT GTCAACAGTT TGTGCAGGGA CTCCTGCTGN 841 TCACTCTCGT CGCTGCGTCC ATGGANTCTG CCATACTCCT CCCGGANAAT CTTGANCANT 901 CTCCGCACCA TGTTGCCCAC NGNGGTCTCN GANGNNTGAN CGGCCGTCAT CCTCCTGNCN 961 NCTCTGNNGA TCAGCTCCNT CANCTCCCCC GCGTTGNTCC NGCNGNGGNC CGNGNNGNNC 1021 TGNNNCANNN NCCCNANGGN CNCCNNNNNN NTTTNNNNNN NNNN // |
Primer: hEIF2B-M_F1 (Pr0935) Region: insert mid (2B4 L314Q) Sequence file: RDB18642_B0Lbc.seq ![]() |
>D07570A4_B0Lb_2_hEIF2B-M_F1_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCC CTCNGGANTG AAGCGTCTCC CTGAGTACCC 61 TCAGGTTGAT GACCTACTTC TGAGAAGGCT TGTTAAAAAA CCAGAGCGTC AACAGGTTCC 121 TACACGAAAG GATTATGGAT CCAAAGTCAG TCTCTTCTCT CACCTACCCC AGTACAGCAG 181 ACAAAACTCT CTGACCCAGT TTATGAGCAT CCCATCCTCT GTGATCCACC CAGCCATGGT 241 GCGACTCGGC CTGCAGTACT CCCAGGGCCT GGTCAGTGGC TCCAATGCCC GGTGTATTGC 301 CCTGCTTCGT GCCTTGCAGC AGGTGATTCA GGATTACACA ACACCGCCTA ATGAAGAACT 361 CTCCAGGGAT CTAGTGAATA AACTAAAACC CTACATGAGC TTCCTGACTC AGTGCCGTCC 421 CCTGTCAGCG AGCATGCACA ACGCCATCAA GTTCCTTAAC AAGGAAATCA CCAGTGTGGG 481 CAGTTCCAAG CGGGAAGAGG AGGCCAAGTC AGAACTTCGA GCAGCCATTG ATCGGTATGT 541 GCAAGAGAAG ATTGTGCAAG CAGCTCAGGC AATTTCACGC TTTGCTTACC AGAAGATCAG 601 TAATGGAGAT GTGATCCTGG TATATGGATG CTCATCTCTG GTATCACGAA TTCTTCAGGA 661 GGCTTGGACA GAGGGCCGGC GGTTTCGGGT GGTAGTGGTG GACAGCCGGC CATGGCTGGA 721 AGGAAGGCAC ACACTACGTT CTCTAGTCCA TGCTGGTGTC CCAGCCTCCT ACCTGCTGAN 781 TCCTGCAGCC TCCTATGTGC TCCCAGANGT TTCCNAGGTG CTATTGGGAG CTCATGCACT 841 CTTGGNCAAC GGGTCTGTGA TGTCACGGGN ANGGACAGCA CAGTTAGCCC TGGTGGCTCG 901 AGCCCATAAT GNACCAGNGC TGGNTTGCTG TGAAACATAC NAGTTCTGTG ANCGTGNGCN 961 NACTGNNGCC TTTGNCTCTA NTGANCTANN NGNNCNTGAN GATCTGNNNN GTAANCGGGG 1021 ANAANNTGNN GCNCTGGCTN NNNNGNNNAN NNNNNNNNCC CNANNNNNNN NNN // |
Primer: hEIF2Bb_R (Pr0902) Region: insert mid (2B2 5', RBS, lacO, T7pro, 2B4 3') Sequence file: RDB18642_B0Lbd.seq ![]() |
>D07570A4_B0Lb_2_hEIF2Bb_R_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNCGNNCGNC NNCCTCCTGC CCTCTCTGCG GNTCAGCTCC 61 ATCAGCTCCC CCGCGTTGCT CCNGCGGTGG TCCGTGATGA TCTGGCGCAG CAACCCTAGG 121 GTCTCCCGAG CCATTTCCTC GGAGCTGCGC GGCCCACCAC CCCGCTTCAG GGTCTCCACG 181 AAGCTCTCGA TCCTCTCTGA CAACTCCGAG CCCTTCGCTG CGGATCCCGG CATATGTATA 241 TCTCCTTCTT ATACTTAACT AATATACTAA GATGGGGAAT TGTTATCCGC TCACAATTCC 301 CCTATAGTGA GTCGTATTAA TTTCGATTAT GCGGCCGTGT ACAATACGAT TACTTTCTGT 361 TCGACTTAAG CATTATGCGG CCGCTCACTG GTCACTGCTC TTGACTCGTA GAACAACAGG 421 TACAGAACTG CAAGGGATCA TCCCCAGCTC CGTGATCACC AGATCCACAA GCTCTGGGGG 481 AGTCACATCA TAGACTAGAT TCAACAACCG TAGGGATGCG TGGTTCTGCC AGTTAGCCAG 541 CGCAACATGT TCTCCCCGCT TACATTGCAG ATCATCAGGG TCATCTAGCT CATTAGAGAC 601 AAAGGCATCA GTCTGCACAC GCTCACAGAA CTTGTATGTT TCACAGCAAA CCAGCACTGG 661 TACATTATGG GCTCGAGCCA CCAGGGCTAA CTGTGCTGTC CCTACCCGTG ACATCACAGA 721 CCCGTTGGCC AAGAGTGCAT GAGCTCCCAA TAGCACCTTG GAAACCTCTG GGAGCACATA 781 NGANGCTGCA NGAATCANCA GGTANGANGC TGGGACACCA GCATGGACTA GAGAACGTAG 841 TGTGTGCCTT CCTTCCAGCC ATGGCCGGCT GTCCACCACT ACCACCCGAA NNCGCCGGCC 901 NNCTGTCNAA NCCTCCTGAA NANNTCNNGA NANCAGANNT GANCATCCAT ATACCNNNAT 961 CACATCTNCN TTANTGATCN NCTGGNANNC AAAGCNNGNA NNTGNNNGAN CTGNNNGNAC 1021 NANCTTCNNN NGNNNNTANN NATNNNNGNN NGNNCNNNNN NNNNN // |
Primer: pGL4pA_R (Pr0664) Region: lacI Sequence file: RDB18642_B0Lbe.seq ![]() |
>D07570A4_B0Lb_2_pGL4pA_R_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNCNGNNGNC TAATGAGTGA GCTAACTTAC NTTAATTGCG 61 TTGCGCTCAC TGCCCGCTTT CNNGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC 121 GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC 181 CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG CCCTGAGAGA GTTGCAGCAA 241 GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTAACGGCGG 301 GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATGT CCGCACCAAC 361 GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC GCCATCTGAT CGTTGGCAAC 421 CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC ATGGTTTGTT GAAAACCGGA 481 CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA ATTTGATTGC GAGTGAGANA 541 TTTATGCCAG CCAGCCAGAN GCAGACGCGC CGAGACAGAA CTTAATGGGN CCGCTAACAG 601 CGCGANTTGC TGGTGACCCN ATGCGANCAG ATGCTCCACG CCCAGTCGCG NANCGTCTTC 661 NTGGGANAAA ATANTACTGN TGANGGGTGN CTGGNCNNAN ANATCNANAA ATAACGNCNG 721 NNANNTTANN GNNNNANCTT NCNCANCNNT GNNATNCTNG NCNTNCNNCN NANANNNANN 781 GNTNNNCCNN NNGANNNNNN NNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Zyryanova, A.F., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP] |
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