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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pETDuet-2B4-2B2_dE310K (#RDB18641)

Bacterial expression vector of human eIF2Bdelta mutant (E310K) and human eIF2Bbeta.

Clone info. Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit delta (eIF2Bdelta) mutant (E310K) and human eukaryotic translation initiation factor 2B subunit beta (eIF2Bbeta) cloned into the pETDuet-1.
Comment Expression was confirmed by the depositor.
Vector backbone pETDuet-1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human EIF2B2 cDNA human EIF2B4 cDNA
Depositor|Developer Ito, Takuhiro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。

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RDB18641 pETDuet-2B4-2B2_dE310K DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pETDuet-2B4-2B2_dE310K was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18641).

Reference section:

Zyryanova, A.F., Kashiwagi, K., Rato, C., Harding, H.P., Crespillo-Casado, A., Perera, L.A., Sakamoto, A., Nishimoto, M., Yonemochi, M., Shirouzu, M., Ito, T., Ron, D., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18641_B0Lbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R (Pr0065)
Region: lacO, RBS, insert 5' (2B4 5')
Sequence file: RDB18641_B0Lba.seq check
>D07570A3_B0Lb_3_pBR322_BamHI_R_D03_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGANTNNC NATAGNNNGA NTGTGAGCGG NNAACAATTC
   61 CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACCATGGGC AGCAGCAGCC
  121 AGGATCCGAA TTCGAGCTCG GCGCGCCTGC AGGTCGACGC TGCTGTGGCC GTGGCTGTTC
  181 GCGAGGACTC GGGATCCGGG ATGAAGGCGG AGCTTCCCCC CGGGCCTGGG GCAGTGGGGA
  241 GGGAAATGAC CAAAGAAGAA AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA
  301 AACGGAAGGA AGAAAAGGGG GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT
  361 GTCAAGTAGG CCCAACCAGA GAACTGCCAG AATCGGGCAT TCAGTTGGGC ACTCCTCGGG
  421 AGAAAGTTCC AGCTGGTCGG AGTAAGGCCG AACTTCGGGC TGAGCGTCGA GCCAAGCAGG
  481 AGGCCGAGCG GGCCCTGAAA CAGGCAAGAA AAGGGGAACA AGGAGGACCA CCTCCTAAGG
  541 CCAGCCCCAG CACAGCTGGA GAAACCCCCT CAGGAGTGAA GCGTCTCCCT GAGTACCCTC
  601 AGGTTGATGA CCTACTTCTG AGAAGGCTTG TTAAAAAACC AGAGCGTCAA CAGGTTCCTA
  661 CACGAAAGGA TTATGGATCC AAAGTCAGTC TCTTCTCTCA CCTACCCCAG TACAGCAGAC
  721 AAAACTCTCT GACCCAGTTT ATGAGCATCC CATCCTCTGT GATCCACCCA GCCATGGTGC
  781 GACTCGGCCT GCAGTACTCC CAGGGCCTGG TCAGTGGCTC CNNTGCCCGG NGTATTGCCC
  841 TGCTTCGTGC CTTGCANCAN GTGATTCANG ATTNNNNNNC ACCGCCTAAT GAANAANTCT
  901 CCNGGGNTCT AGNGAATAAA CTAAANNCCT ACNNGANCTT NCTGANTCAG NGNCGTCCCN
961 NGTCANCGAG NATGNNNNNN NNNN
//
Primer: T7 terminator (Pr0155)
Region: insert 3' (2B2 3')
Sequence file: RDB18641_B0Lbb.seq check
>D07570A3_B0Lb_3_T7 terminator_E03_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCNNN NANNNNANTC NNTGTGCTGG CGTTCAAATT TCGCAGCAGC
   61 GGTTTCTTTA CCAGACTCNN GGGTACCGAC GTCTCATAAA ACATGATCAT CAGGATGGTA
  121 GAGTTCACTC ATCAGGCGGT AGATGTAGGA AGGTGCATTC CCACCAATGT TGGAGATAAA
  181 GAGGGTAATG AGCTCTGGGG GAACGTAGTC AAACACAGGG CAATGCACGC TGACCTTCTC
  241 CAGAATGTCC CCTTCTGTGA ATGGCAGGAC TTCTTCAGGA GCCACAAACT TATGAAATGA
  301 GTCTTCTTCA TTGGGGAACT GTGGAGAAAG TTTGAACATA GGTGCACAGA CGATGAGTGG
  361 GGTGGAATGG TGTTTTGCTG CCAGTGCCAG AGTGTGAGTT CCTGTCACAG CTCTCAGGGC
  421 CCCATTGGCC AGGATGGTCT TCGTGCCAAT GATCACCTTG TTGACTCTTG ACATAACGGC
  481 AAAAATGGCA GCATCAGTCA TGACAGTTGT CTCAATACCT GCTTTGGACA AATTCACAGC
  541 CATTTCATGA CCCTGGCAGA AAGGAGCACA CTCTGCTACA ATGACATGGA ATTTCCTCTT
  601 TCGGGCAGCC TCTTTGAGGA AGGCCTCTAC TGTTCGGGAG AAGCCAATGG TCATGATCAC
  661 CTCATTGGAG TGAATGTGCT CCAGAGCCTG GGCTGCAATG TTCTCCATTG TCCCTTCCAG
  721 CTCCACTAGC AGCTCATTAA TCGCCTCAAT GATGTTGGAC TGGAGTTGGG CATAATGNAA
  781 GCTGAAATCC TCGTTNANGC CTCCGGATGT CAACAGTTTG TGCAGGGACT CCTGCTGATC
  841 ACTCTCGTCG CTGCGNCCAT GGAGTCTGCC ATACTCCTCC CGGATAATCT TGANCANTCT
  901 CCGCANNATG TNGNCCACGG NGGTCTCNGA NGGNTGANCG GCNNNCNTCC NNCTGNCNNC
961 TCTGNNNATC ANCTCCNTCA NCTNCCCCNC GNNGNTNCNN CNGNNNNNNN NNN
//
Primer: hEIF2B-M_F1 (Pr0935)
Region: insert mid (2B4 E310K)
Sequence file: RDB18641_B0Lbc.seq check
>D07570A3_B0Lb_3_hEIF2B-M_F1_F03_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CCNNNNNNNT GAAGCGTCTC CCTGANTACC
   61 CTCAGGTTGA TGACCTACTT CTGAGAAGGC TTGTTAAAAA ACCAGAGCGT CAACAGGTTC
  121 CTACACGAAA GGATTATGGA TCCAAAGTCA GTCTCTTCTC TCACCTACCC CAGTACAGCA
  181 GACAAAACTC TCTGACCCAG TTTATGAGCA TCCCATCCTC TGTGATCCAC CCAGCCATGG
  241 TGCGACTCGG CCTGCAGTAC TCCCAGGGCC TGGTCAGTGG CTCCAATGCC CGGTGTATTG
  301 CCCTGCTTCG TGCCTTGCAG CAGGTGATTC AGGATTACAC AACACCGCCT AATGAAGAAC
  361 TCTCCAGGGA TCTAGTGAAT AAACTAAAAC CCTACATGAG CTTCCTGACT CAGTGCCGTC
  421 CCCTGTCAGC GAGCATGCAC AACGCCATCA AGTTCCTTAA CAAGGAAATC ACCAGTGTGG
  481 GCAGTTCCAA GCGGGAAGAG GAGGCCAAGT CAGAACTTCG AGCAGCCATT GATCGGTATG
  541 TGCAAAAGAA GATTGTGCTA GCAGCTCAGG CAATTTCACG CTTTGCTTAC CAGAAGATCA
  601 GTAATGGAGA TGTGATCCTG GTATATGGAT GCTCATCTCT GGTATCACGA ATTCTTCAGG
  661 AGGCTTGGAC AGAGGGCCGG CGGTTTCGGG TGGTAGTGGT GGACAGCCGG CCATGGCTGG
  721 AAGGAAGGCA CACACTACGT TCTCTAGTCC ATGCTGGTGT CCCAGCCTCC TACCTGCTGA
  781 TTCCTGCAGC CTCCTATGTG CTCNNNNANG TTTCCAAGGT GCTATTGGGA NCTCATGCAC
  841 TCTTGGCCNA CGGGTCTGTG ATGTCACGGG TANGGANAGC ACAGTTAGCC CTGNTGGCTC
  901 GAGCCCATAA TGNACCAGNG CTGGTTTGCT GTGAAACNTA CAAGTTCTGT GANCGTGTGC
  961 ANNNTNNTNN CTTTNGTNTN NAATGANCTA NATGANCNTN NNNNATCTGC NNNGNNANNN
1021 NNNNNNN
//
Primer: hEIF2Bb_R (Pr0902)
Region: insert mid (2B2 5', RBS, lacO, T7pro, 2B4 3')
Sequence file: RDB18641_B0Lbd.seq check
>D07570A3_B0Lb_3_hEIF2Bb_R_G03_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT CCTCCTGCCC TCTCTGCGGN NCAGCTCCNT
   61 CAGCTCCCCC GCGTTGCTCC NGCGGTGGTC CGTGATGATC TGGCGCAGCA ACCCTAGGGT
  121 CTCCCGAGCC ATTTCCTCGG AGCTGCGCGG CCCACCACCC CGCTTCAGGG TCTCCACGAA
  181 GCTCTCGATC CTCTCTGACA ACTCCGAGCC CTTCGCTGCG GATCCCGGCA TATGTATATC
  241 TCCTTCTTAT ACTTAACTAA TATACTAAGA TGGGGAATTG TTATCCGCTC ACAATTCCCC
  301 TATAGTGAGT CGTATTAATT TCGATTATGC GGCCGTGTAC AATACGATTA CTTTCTGTTC
  361 GACTTAAGCA TTATGCGGCC GCTCACTGGT CACTGCTCTT GACTCGTAGA ACAACAGGTA
  421 CAGAACTGCA AGGGATCATC CCCAGCTCCG TGATCACCAG ATCCACAAGC TCTGGGGGAG
  481 TCACATCATA GACTAGATTC AACAACCGTA GGGATGCGTG GTTCTGCCAG TTAGCCAGCG
  541 CAACATGTTC TCCCCGCTTA CATTGCAGAT CATCAGGGTC ATCTAGCTCA TTAGAGACAA
  601 AGGCATCAGT CTGCACACGC TCACAGAACT TGTATGTTTC ACAGCAAACC AGCACTGGTA
  661 CATTATGGGC TCGAGCCACC AGGGCTAACT GTGCTGTCCC TACCCGTGAC ATCACAGACC
  721 CGTTGGCCAA GAGTGCATGA GCTCCCNATA GCACCTTGGA AACCTCTGGG AGCACATAGG
  781 AGGCTGCAGG AATCANCANG TANGANGCTG GGACACCAGC ATGNACTANA GAACGTAGTG
  841 TGTGCCTTNC TTNCAGCCAT GGCCGGNTGN NCNCCACTAC NACCCGAAAC CGCCGGCCCT
  901 CTGTNCNNNC CTNCTGAAGA ATTCGTGATA CCAGANATGA NCATNCNNAT NNNNNNNTNA
  961 CATCTCNNTN NCTGATCTTC TGGNNNNNAA AGCGNNNANN TGNNNNANCT NNTANCNNNN
1021 NNNNNN
//
Primer: pGL4pA_R (Pr0664)
Region: lacI
Sequence file: RDB18641_B0Lbe.seq check
>D07570A3_B0Lb_3_pGL4pA_R_H03_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC CNNGNGNNNN NNNAGTGAGC TAACTTACNT TAATTGCGTT
   61 GCGCTCACTG CCCGNNNNNN NNNNNGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG
  121 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA
  181 GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC
  241 GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA
  301 TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATGTCC GCACCAACGC
  361 GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA
  421 GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA
  481 TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT
  541 TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG
  601 CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT
  661 GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA
  721 CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCNGCGGA TAGTTAATGA
  781 TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACNG GCTTCGANGC
  841 CGCTTCNTTC TACCATCGAC ACCACCACGC TGGCACCCAG TNGATCNGCG CGAGATTTAA
  901 TCGCCGCGAC NATTTGNNAN GGCGCGNGNA GGGCNNNANT GNANGTGGNA NNNCNNATCA
  961 NCNACNACNG NTNNNCCNNC ANNNGNNGNG CCNNGCNGNN NNGNNNTGNN NNNNANCTNC
1021 NGCCNNCNNN NNNTNNNNNN TTTTNNNNNN NNTTNNNNNN NANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Zyryanova, A.F., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP]