Bacterial expression vector of human eIF2Bdelta mutant (E310K) and human eIF2Bbeta.
Clone info. | Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit delta (eIF2Bdelta) mutant (E310K) and human eukaryotic translation initiation factor 2B subunit beta (eIF2Bbeta) cloned into the pETDuet-1. |
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Comment | Expression was confirmed by the depositor. |
Vector backbone | pETDuet-1 (plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Ampicillin |
Growth conditions | LB+Ampicillin, 37oC |
Gene/insert name | human EIF2B2 cDNA human EIF2B4 cDNA |
Depositor|Developer | Ito, Takuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18641 | pETDuet-2B4-2B2_dE310K | DNA solution |
Materials & Methods section:
The pETDuet-2B4-2B2_dE310K was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18641). |
Reference section:
Zyryanova, A.F., Kashiwagi, K., Rato, C., Harding, H.P., Crespillo-Casado, A., Perera, L.A., Sakamoto, A., Nishimoto, M., Yonemochi, M., Shirouzu, M., Ito, T., Ron, D., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18641_B0Lbp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pBR322_BamHI_R (Pr0065) Region: lacO, RBS, insert 5' (2B4 5') Sequence file: RDB18641_B0Lba.seq ![]() |
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>D07570A3_B0Lb_3_pBR322_BamHI_R_D03_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGANTNNC NATAGNNNGA NTGTGAGCGG NNAACAATTC 61 CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACCATGGGC AGCAGCAGCC 121 AGGATCCGAA TTCGAGCTCG GCGCGCCTGC AGGTCGACGC TGCTGTGGCC GTGGCTGTTC 181 GCGAGGACTC GGGATCCGGG ATGAAGGCGG AGCTTCCCCC CGGGCCTGGG GCAGTGGGGA 241 GGGAAATGAC CAAAGAAGAA AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA 301 AACGGAAGGA AGAAAAGGGG GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT 361 GTCAAGTAGG CCCAACCAGA GAACTGCCAG AATCGGGCAT TCAGTTGGGC ACTCCTCGGG 421 AGAAAGTTCC AGCTGGTCGG AGTAAGGCCG AACTTCGGGC TGAGCGTCGA GCCAAGCAGG 481 AGGCCGAGCG GGCCCTGAAA CAGGCAAGAA AAGGGGAACA AGGAGGACCA CCTCCTAAGG 541 CCAGCCCCAG CACAGCTGGA GAAACCCCCT CAGGAGTGAA GCGTCTCCCT GAGTACCCTC 601 AGGTTGATGA CCTACTTCTG AGAAGGCTTG TTAAAAAACC AGAGCGTCAA CAGGTTCCTA 661 CACGAAAGGA TTATGGATCC AAAGTCAGTC TCTTCTCTCA CCTACCCCAG TACAGCAGAC 721 AAAACTCTCT GACCCAGTTT ATGAGCATCC CATCCTCTGT GATCCACCCA GCCATGGTGC 781 GACTCGGCCT GCAGTACTCC CAGGGCCTGG TCAGTGGCTC CNNTGCCCGG NGTATTGCCC 841 TGCTTCGTGC CTTGCANCAN GTGATTCANG ATTNNNNNNC ACCGCCTAAT GAANAANTCT 901 CCNGGGNTCT AGNGAATAAA CTAAANNCCT ACNNGANCTT NCTGANTCAG NGNCGTCCCN 961 NGTCANCGAG NATGNNNNNN NNNN // |
Primer: T7 terminator (Pr0155) Region: insert 3' (2B2 3') Sequence file: RDB18641_B0Lbb.seq ![]() |
>D07570A3_B0Lb_3_T7 terminator_E03_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNCNNN NANNNNANTC NNTGTGCTGG CGTTCAAATT TCGCAGCAGC 61 GGTTTCTTTA CCAGACTCNN GGGTACCGAC GTCTCATAAA ACATGATCAT CAGGATGGTA 121 GAGTTCACTC ATCAGGCGGT AGATGTAGGA AGGTGCATTC CCACCAATGT TGGAGATAAA 181 GAGGGTAATG AGCTCTGGGG GAACGTAGTC AAACACAGGG CAATGCACGC TGACCTTCTC 241 CAGAATGTCC CCTTCTGTGA ATGGCAGGAC TTCTTCAGGA GCCACAAACT TATGAAATGA 301 GTCTTCTTCA TTGGGGAACT GTGGAGAAAG TTTGAACATA GGTGCACAGA CGATGAGTGG 361 GGTGGAATGG TGTTTTGCTG CCAGTGCCAG AGTGTGAGTT CCTGTCACAG CTCTCAGGGC 421 CCCATTGGCC AGGATGGTCT TCGTGCCAAT GATCACCTTG TTGACTCTTG ACATAACGGC 481 AAAAATGGCA GCATCAGTCA TGACAGTTGT CTCAATACCT GCTTTGGACA AATTCACAGC 541 CATTTCATGA CCCTGGCAGA AAGGAGCACA CTCTGCTACA ATGACATGGA ATTTCCTCTT 601 TCGGGCAGCC TCTTTGAGGA AGGCCTCTAC TGTTCGGGAG AAGCCAATGG TCATGATCAC 661 CTCATTGGAG TGAATGTGCT CCAGAGCCTG GGCTGCAATG TTCTCCATTG TCCCTTCCAG 721 CTCCACTAGC AGCTCATTAA TCGCCTCAAT GATGTTGGAC TGGAGTTGGG CATAATGNAA 781 GCTGAAATCC TCGTTNANGC CTCCGGATGT CAACAGTTTG TGCAGGGACT CCTGCTGATC 841 ACTCTCGTCG CTGCGNCCAT GGAGTCTGCC ATACTCCTCC CGGATAATCT TGANCANTCT 901 CCGCANNATG TNGNCCACGG NGGTCTCNGA NGGNTGANCG GCNNNCNTCC NNCTGNCNNC 961 TCTGNNNATC ANCTCCNTCA NCTNCCCCNC GNNGNTNCNN CNGNNNNNNN NNN // |
Primer: hEIF2B-M_F1 (Pr0935) Region: insert mid (2B4 E310K) Sequence file: RDB18641_B0Lbc.seq ![]() |
>D07570A3_B0Lb_3_hEIF2B-M_F1_F03_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CCNNNNNNNT GAAGCGTCTC CCTGANTACC 61 CTCAGGTTGA TGACCTACTT CTGAGAAGGC TTGTTAAAAA ACCAGAGCGT CAACAGGTTC 121 CTACACGAAA GGATTATGGA TCCAAAGTCA GTCTCTTCTC TCACCTACCC CAGTACAGCA 181 GACAAAACTC TCTGACCCAG TTTATGAGCA TCCCATCCTC TGTGATCCAC CCAGCCATGG 241 TGCGACTCGG CCTGCAGTAC TCCCAGGGCC TGGTCAGTGG CTCCAATGCC CGGTGTATTG 301 CCCTGCTTCG TGCCTTGCAG CAGGTGATTC AGGATTACAC AACACCGCCT AATGAAGAAC 361 TCTCCAGGGA TCTAGTGAAT AAACTAAAAC CCTACATGAG CTTCCTGACT CAGTGCCGTC 421 CCCTGTCAGC GAGCATGCAC AACGCCATCA AGTTCCTTAA CAAGGAAATC ACCAGTGTGG 481 GCAGTTCCAA GCGGGAAGAG GAGGCCAAGT CAGAACTTCG AGCAGCCATT GATCGGTATG 541 TGCAAAAGAA GATTGTGCTA GCAGCTCAGG CAATTTCACG CTTTGCTTAC CAGAAGATCA 601 GTAATGGAGA TGTGATCCTG GTATATGGAT GCTCATCTCT GGTATCACGA ATTCTTCAGG 661 AGGCTTGGAC AGAGGGCCGG CGGTTTCGGG TGGTAGTGGT GGACAGCCGG CCATGGCTGG 721 AAGGAAGGCA CACACTACGT TCTCTAGTCC ATGCTGGTGT CCCAGCCTCC TACCTGCTGA 781 TTCCTGCAGC CTCCTATGTG CTCNNNNANG TTTCCAAGGT GCTATTGGGA NCTCATGCAC 841 TCTTGGCCNA CGGGTCTGTG ATGTCACGGG TANGGANAGC ACAGTTAGCC CTGNTGGCTC 901 GAGCCCATAA TGNACCAGNG CTGGTTTGCT GTGAAACNTA CAAGTTCTGT GANCGTGTGC 961 ANNNTNNTNN CTTTNGTNTN NAATGANCTA NATGANCNTN NNNNATCTGC NNNGNNANNN 1021 NNNNNNN // |
Primer: hEIF2Bb_R (Pr0902) Region: insert mid (2B2 5', RBS, lacO, T7pro, 2B4 3') Sequence file: RDB18641_B0Lbd.seq ![]() |
>D07570A3_B0Lb_3_hEIF2Bb_R_G03_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT CCTCCTGCCC TCTCTGCGGN NCAGCTCCNT 61 CAGCTCCCCC GCGTTGCTCC NGCGGTGGTC CGTGATGATC TGGCGCAGCA ACCCTAGGGT 121 CTCCCGAGCC ATTTCCTCGG AGCTGCGCGG CCCACCACCC CGCTTCAGGG TCTCCACGAA 181 GCTCTCGATC CTCTCTGACA ACTCCGAGCC CTTCGCTGCG GATCCCGGCA TATGTATATC 241 TCCTTCTTAT ACTTAACTAA TATACTAAGA TGGGGAATTG TTATCCGCTC ACAATTCCCC 301 TATAGTGAGT CGTATTAATT TCGATTATGC GGCCGTGTAC AATACGATTA CTTTCTGTTC 361 GACTTAAGCA TTATGCGGCC GCTCACTGGT CACTGCTCTT GACTCGTAGA ACAACAGGTA 421 CAGAACTGCA AGGGATCATC CCCAGCTCCG TGATCACCAG ATCCACAAGC TCTGGGGGAG 481 TCACATCATA GACTAGATTC AACAACCGTA GGGATGCGTG GTTCTGCCAG TTAGCCAGCG 541 CAACATGTTC TCCCCGCTTA CATTGCAGAT CATCAGGGTC ATCTAGCTCA TTAGAGACAA 601 AGGCATCAGT CTGCACACGC TCACAGAACT TGTATGTTTC ACAGCAAACC AGCACTGGTA 661 CATTATGGGC TCGAGCCACC AGGGCTAACT GTGCTGTCCC TACCCGTGAC ATCACAGACC 721 CGTTGGCCAA GAGTGCATGA GCTCCCNATA GCACCTTGGA AACCTCTGGG AGCACATAGG 781 AGGCTGCAGG AATCANCANG TANGANGCTG GGACACCAGC ATGNACTANA GAACGTAGTG 841 TGTGCCTTNC TTNCAGCCAT GGCCGGNTGN NCNCCACTAC NACCCGAAAC CGCCGGCCCT 901 CTGTNCNNNC CTNCTGAAGA ATTCGTGATA CCAGANATGA NCATNCNNAT NNNNNNNTNA 961 CATCTCNNTN NCTGATCTTC TGGNNNNNAA AGCGNNNANN TGNNNNANCT NNTANCNNNN 1021 NNNNNN // |
Primer: pGL4pA_R (Pr0664) Region: lacI Sequence file: RDB18641_B0Lbe.seq ![]() |
>D07570A3_B0Lb_3_pGL4pA_R_H03_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNC CNNGNGNNNN NNNAGTGAGC TAACTTACNT TAATTGCGTT 61 GCGCTCACTG CCCGNNNNNN NNNNNGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 121 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA 181 GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC 241 GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA 301 TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATGTCC GCACCAACGC 361 GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA 421 GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA 481 TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT 541 TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG 601 CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT 661 GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA 721 CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCNGCGGA TAGTTAATGA 781 TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACNG GCTTCGANGC 841 CGCTTCNTTC TACCATCGAC ACCACCACGC TGGCACCCAG TNGATCNGCG CGAGATTTAA 901 TCGCCGCGAC NATTTGNNAN GGCGCGNGNA GGGCNNNANT GNANGTGGNA NNNCNNATCA 961 NCNACNACNG NTNNNCCNNC ANNNGNNGNG CCNNGCNGNN NNGNNNTGNN NNNNANCTNC 1021 NGCCNNCNNN NNNTNNNNNN TTTTNNNNNN NNTTNNNNNN NANNNNNNNN NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Zyryanova, A.F., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP] |
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