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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pETDuet-eIF2Bd-eIF2Bb (#RDB17723)

Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit beta (eIF2Bbeta) and human eukaryotic translation initiation factor 2B subunit delta (eIF2Bdelta).

Alternative name eIF2Bbeta-delta
Clone info. Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit beta (eIF2Bbeta) and human eukaryotic translation initiation factor 2B subunit delta (eIF2Bdelta) cloned into the pETDuet-1.
Comment Expression was confirmed by the depositor.
Vector backbone pETDuet-1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human EIF2B2 cDNA human EIF2B4 cDNA
Depositor|Developer Ito, Takuhiro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。

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RDB17723 pETDuet-eIF2Bd-eIF2Bb DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pETDuet-eIF2Bd-eIF2Bb was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17723).

Reference section:

Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17723_B0Adp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBE322_BamHI_R (Pr0065)
Region: lacO, RBS, insert1 5'
Sequence file: RDB17723_B0Ada.seq check
>D06721A2_B0Ad_2_pBR322_BamHI_R_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNNNNNNNN TCNCTATNNN NNNAANTGTG AGCGGANAAC
   61 AATTCCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGGCAGCAG
  121 CAGCCAGGAT CCGAATTCGA GCTCGGCGCG CCTGCAGGTC GACGCTGCTG TGGCCGTGGC
  181 TGTTCGCGAG GACTCGGGAT CCGGGATGAA GGCGGAGCTT CCCCCCGGGC CTGGGGCAGT
  241 GGGGAGGGAA ATGACCAAAG AAGAAAAGCT GCAGCTTCGG AAGGAAAAGA AACAGCAGAA
  301 GAAGAAACGG AAGGAAGAAA AGGGGGCAGA ACCAGAGACT GGCTCTGCTG TATCTGCAGC
  361 CCAATGTCAA GTAGGCCCAA CCAGAGAACT GCCAGAATCG GGCATTCAGT TGGGCACTCC
  421 TCGGGAGAAA GTTCCAGCTG GTCGGAGTAA GGCCGAACTT CGGGCTGAGC GTCGAGCCAA
  481 GCAGGAGGCC GAGCGGGCCC TGAAACAGGC AAGAAAAGGG GAACAAGGAG GACCACCTCC
  541 TAAGGCCAGC CCCAGCACAG CTGGAGAAAC CCCCTCAGGA GTGAAGCGTC TCCCTGAGTA
  601 CCCTCAGGTT GATGACCTAC TTCTGANAAG GCTTGTTAAA AAACCAGAGC GTCAACAGGT
  661 TCCTACACGA AAGGATTATG GATCCAAAGT CAGTCTCTTC TCTCACCTAC CCCAGTACAG
  721 CAGACAAAAC TCTCTGANCC AGTTTATGAG CATCCCATCC TCTGTGATCC ACCCAGCCNT
  781 GGTGCGACTC GGCCTGCAGT ACTCCCAGGG CCTGGTCAGT GGCTCCAATG CCCGGNGTAN
  841 TGNCCNGCTT NCGNGNCNNG CANCNNNGNN NTCNNGANTA NNCNACACCG NCNTAANGNA
  901 AGAACTCNNC NGGGNTCTAG TGNANNNNTN AAANCCTNNN TGANNNNNNN NNANNCNNNN
961 NNNNNCCNNN NCNNNNNNNN NN
//
Primer: hEIF2Bb_R (Pr0902)
Region: insert2 5', RBS, lacO, T7 pro, insert1 3'
Sequence file: RDB17723_B0Adb.seq check
>D06721A2_B0Ad_2_hEIF2Bb_R_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGCCCTCT CNGNNNNNNN
   61 NNNNNNNNNG CTCCCCCGCG TTGNNNNNNN GGTGGTCCGT GATGATCTGG CGCAGCAACC
  121 CTAGNNNNNN CCGAGCCATT TCCTCGGAGC TGCGCGGCCC ACCACCCCGC TTCAGGGTCT
  181 CCACGAAGCT CTCGATCCTC TCTGACAACT CCGAGCCCTT CGCTGCGGAT CCCGGCATAT
  241 GTATATCTCC TTCTTATACT TAACTAATAT ACTAAGATGG GGAATTGTTA TCCGCTCACA
  301 ATTCCCCTAT AGTGAGTCGT ATTAATTTCG ATTATGCGGC CGTGTACAAT ACGATTACTT
  361 TCTGTTCGAC TTAAGCATTA TGCGGCCGCT CACTGGTCAC TGCTCTTGAC TCGTAGAACA
  421 ACAGGTACAG AACTGCAAGG GATCATCCCC AGCTCCGTGA TCACCAGATC CACAAGCTCT
  481 GGGGGAGTCA CATCATAGAC TAGATTCAAC AACCGTAGGG ATGCGTGGTT CTGCCAGTTA
  541 GCCAGCGCAA CATGTTCTCC CCGCTTACAT TGCAGATCAT CAGGGTCATC TAGCTCATTA
  601 GAGACAAAGG CATCAGTCTG CACACGCTCA CAGAACTTGT ATGTTTNNNN NNAAACCANC
  661 ACTGGTACAT TATGGGCTCG AGCCACCAGG GCTAACTGTG CTGTCCCTAC CCGTGACATC
  721 ACAGACCCGT TGGCCAANAN TGCATGAGCT CCCAATAGCA CCTTGGAAAC CTCTGGGAGC
  781 ANNTANGANG CTGCANGAAT CANCAGGTAG GANGNTGGGA CNNNAGCATG GACTANNNAN
  841 NGTAGTGTGT GNCNTTCCTT CNNGCCATGG CCGGCTGTCC ANCANNNNCC NNCNGAANNC
  901 GNCNGNCCTC TGNNNNNNNC TNNNNNNANA NNNNNNNGAT NNCNNNAGAT GAGCNTCNNN
  961 NNTNCCNNNN ATCANNNNNN NCNNTTNNNN GANNNTNNTG GNNNANNCAA AAGNNNNGNA
 1021 AANNNGNCNT GNANCNNNNN NNCNNCNANN NNNNCNNCNT NGNNNNNNNA NCNNNNNNNN
1081 NANNNGNNNN NNNNNN
//
Primer: T7 terminator (Pr0155)
Region: insert2 3'
Sequence file: RDB17723_B0Adc.seq check
>D06721_A2_B0Ad_2_T7terminator_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGNGC NNNNANNNGA NTCNNTGTGC TGGCGTTCNA ATTTCGCAGC
   61 AGCGGTTTCT TTACCAGACT CGAGGGTACC GACGTCTCAT AAAACATGAT CATCAGGATG
  121 GTAGAGTTCA CTCATCAGGC GGTAGATGTA GGAAGGTGCA TTCCCACCAA TGTTGGAGAT
  181 AAAGAGGGTA ATGAGCTCTG GGGGAACGTA GTCAAACACA GGGCAATGCA CGCTGACCTT
  241 CTCCAGAATG TCCCCTTCTG TGAATGGCAG GACTTCTTCA GGAGCCACAA ACTTATGAAA
  301 TGAGTCTTCT TCATTGGGGA ACTGTGGAGA AAGTTTGAAC ATAGGTGCAC AGACGATGAG
  361 TGGGGTGGAA TGGTGTTTTG CTGCCAGTGC CAGAGTGTGA GTTCCTGTCA CAGCTCTCAG
  421 GGCCCCATTG GCCAGGATGG TCTTCGTGCC AATGATCACC TTGTTGACTC TTGACATAAC
  481 GGCAAAAATG GCAGCATCAG TCATGACAGT TGTCTCAATA CCTGCTTTGG ACAAATTCAC
  541 AGCCATTTCA TGACCCTGGC AGAAAGGAGC ACACTCTGCT ACAATGACAT GGAATTTCCT
  601 CTTTCGGGCA GCCTCTTTGA GGAAGGCCTC TACTGTTCGG GAGAAGCCAA TGGTCATGAT
  661 CACCTCATTG GAGTGAATGT GCTCCAGAGC CTGGGCTGCA ATGTTCTCCA TTGTCCCTTC
  721 CAGCTCCACT AGCAGCTCAT TAATCGCCTC NATGATGTTG GACTGGAGTT GGGCNTAATG
  781 GAAGCTGAAA TCCTCGTTTA NGCCTCCGGA NGNCAACAGT TTGTGCAGGG ACTCCTGCTG
  841 ATCACTCTCG TCGCTGCNNC CATGGANTCT GCCATACTCC NNCNGGATAA TCTNGANCAN
  901 TCNNCGCACN NNNTNGCCCA CGGNNGNTCT CGGANGGCTG ANCNGCNNNN ATNNNNNNNN
961 CNNNNNNNNN GNATCNNNNN CNNNNNNNNC CNNCNNNNNN NNNN
//
Primer: pET_lacIpro_F2 (Pr0646)
Region: lacI pro, lacI
Sequence file: RDB17723_B0Add.seq check
>D06721_A2_B0Ad_2_pET_lacIpro_F2_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGCGGT TGCTGGCGCC TATATCGCCG ANATCNCCGA
   61 TGGGGAAGAN CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT
  121 GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC
  181 GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA
  241 TAAGGGAGAG CGTCGAGATC CCGGACACCA TCGAATGGCG CAAAACCTTT CGCGGTATGG
  301 CATGATAGCG CCCGGAAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT
  361 ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG
  421 CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT
  481 ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG
  541 CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG
  601 CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT
  661 GTAAAGCGNC GGTGCACAAT CTTCTCGCGC ANCGCGTCAG TGGGCTGATC ATTAACTATC
  721 CGCTGGATGA NCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT
  781 TTCTTGATGT CTCTGANCAG ACACCCATCA ACAGTATTAN TTTCTCCCAT GAAGANNGTA
  841 CGCGACTNNN CGTGGNNCAT CTGGNCGCAT NGGGNCACCA GCAAATCGCG CTGTTAGCGG
  901 GNCCNTTAAN TTCTGTCNCN GCGCNNNTGC NNNNGGCTGG CTGGCATAAA TATNNNANNN
961 GNNANTCNAA TTNNNNNNAN NNNGGNNNNG GNNANGGCNN NNNGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]