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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-bsr-ss-EGFP

Retroviral expression vector of secretory form of EGFP.

Catalog number RDB17363
Resource name pMRX-bsr-ss-EGFP
Clone info. Retroviral expression vector of secretory form of EGFP, tagged with the signal sequence from mouse calreticulin (ss) at N-terminus.
Vector backbone pMRX-IRES-bsr (plasmid)
Selectable markers Amp^r (E. coli), Blasticidin^r (mammalian cells).
Gene/insert name mouse Calr cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Calr

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)。
          付加的提供条件:
          pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17363 pMRX-bsr-ss-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17363_A9Lgp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F (Pr0352)
          Region: pol region, SS, EGFP 5'
          Sequence file: RDB17363_A9Lga.seq check
          >D06377A7_A9Lg_2_MMLV-pol_F_A07_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNGGNAT CGCAGCTTGG NNNCNCGCCG CCCNCGTGAA GGCTGCCGAC
             61 CCCGGGGGTG GACCATCCTC TAGACTGCCG GATCTGCCAC CATGCTCCTT TCGGTGCCGC
            121 TCCTGCTTGG CCTCCTCGGC CTGGCCGCCG CAAGATCCAT GGTGAGCAAG GGCGAGGAGC
            181 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT
            241 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA
            301 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG
            361 GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG
            421 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA
            481 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG
            541 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA
            601 GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA
            661 TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC
            721 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC
            781 TGANCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGANTTC GTGACCGCCG
            841 CCGGNNTCAC TCTCGGCATG GACGANCTGN ACAAGGGNTC CNNANCGGNC GCGGNAACAA
            901 TTGTTNACTN NNTNNNGCTA GCNACNGTTT CCNNCTANCG GGNNCNATTN CGCCCCCCCC
          961 NNCCNANNNN NANNGNNNGN NNNCNNNNNN NNNN
          //
          Primer: IRES-R (Pr0037)
          Region: EGFP 3'
          Sequence file: RDB17363_A9Lgb.seq check
          >D06377A7_A9Lg_2_IRES-R_B07_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTTA GGGGGGGGGG GCGGNNNTGN TCCCGCNAGA
             61 GGGAAACCGT TGCTAGCTTA AGTTAGTTAA CAATTGTTAC CGCGGCCGCT TAGGATCCCT
            121 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA
            181 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT
            241 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
            301 GCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT
            361 TCTGCTTGTC GGCCATGATA TANACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC
            421 CCAGGATGTT GCCGTCCTCC TTGAAGTCNA TGCCCTTCAG CTCGATGCGG TTCACCAGGG
            481 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAANAANA
            541 TGGTGCGCTC CTGGACGTAN CCTTCGGGCA TGGCGGACTT GAANAAGTCN TGCTGCTTCA
            601 TGTGGTCGGG GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG
            661 GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC ANATGAACTT CAGGGTCAGC TTGCCGTAGG
            721 TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA
            781 GCTCGACCAG GANGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGATC
            841 TTGCGGCGGC CANGCCGANG NNGNCNAGCA GGNNCGGCNC CNAAAGGANC ATGGNGNNAN
            901 ATNCGGNANN CTANANGNNG GNCCACCCCC NGGGNCGGCA GCNNTNNCGT NNNNGNGNGN
          961 ATNCNAGCTG CNATNNCGNN TANTTTNANG NNNNNGGGGG TNNNNANNNN NNNNNN
          //
          Primer: IRES-F (Pr0036)
          Region: IRES, bsr, 3' LTR
          Sequence file: RDB17363_A9Lgc.seq check
          >D06377A7_A9Lg_2_IRES-F_C07_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGT CGAGGTNNNN NNNNNNTCTA GGCCCCCCGA ACCACGGGGN
             61 CGTGGTTTTC CTTTGAAAAA CACGATAATA CCATGGTCAT GAAAACATTT AACATTTCTC
            121 AACAAGATCT AGAATTAGTA GAAGTAGCGA CAGAGAAGAT TACAATGCTT TATGAGGATA
            181 ATAAACATCA TGTGGGAGCG GCAATTCGTA CGAAAACAGG AGAAATCATT TCGGCAGTAC
            241 ATATTGAAGC GTATATAGGA CGAGTAACTG TTTGTGCAGA AGCCATTGCG ATTGGTAGTG
            301 CAGTTTCGAA TGGACAAAAG GATTTTGACA CGATTGTAGC TGTTAGACAC CCTTATTCTG
            361 ACGAAGTAGA TAGAAGTATT CGAGTGGTAA GTCCTTGTGG TATGTGTAGG GAGTTGATTT
            421 CAGACTATGC ACCAGATTGT TTTGTGTTAA TAGAAATGAA TGGCAAGTTA GTCAAAACTA
            481 CGATTGAAGA ACTCATTCCA CTCAAATATA CCCGAAATTA AAAGTCGACG ATAAAATAAA
            541 AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA
            601 AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAGA
            661 AGTTCAGATC AAGGTCAGGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG
            721 TGGTAAGCAG TTCCTGCCCC GGCTCANGGC CNAGAACAGA TGGAACAGCT GAATATGGGC
            781 CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC ANGGCCNAGA ACAGATGGTC
            841 CCCAGATGCG GTCCAGCCCT CAGCAGTTTC TAGAGAANCA TCANANGTTT CCNGGGNGNC
            901 CCAAGGANCT GAANTGANCC TGNGCCTTAT TTGAACTNAC CNATCAGTTC GCTTCNCGCT
            961 NCNNTTCNNN NGCTTCNGNT NCNNNANCNC NNNAAAAGAN NNNNNNNNCN NNNNNNNNGG
          1021 NNNNNNNNNN
          //
          Primer: CMV enh_F1 (Pr0652)
          Region: CMV en, 5' LTR, MMLV psi
          Sequence file: RDB17363_A9Lgd.seq check
          >D06377A7_A9Lg_2_CMVenh_F1_D07_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTNN NNNNNNNTGN NCTTATGGGA NTTTCCTACT TGGCAGNANN
             61 NCTACGTATT AGTCATCGCT ATTACCATGC TAGAGAACCA TCAGATGTTT CCAGGGTGCC
            121 CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT
            181 TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC
            241 GCGCCAGTCC TCCGATTGAC TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG
            301 CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC
            361 TACCCGTCAG CGGGGGTCTT TCATTTGGGG GCTCGTCCGG GATCGGGAGA CCCCTGCCCA
            421 GGGACCACCG ACCCACCACC GGGAGGTAAG CTGGCCAGCA ACTTATCTGT GTCTGTCCGA
            481 TTGTCTAGTG TCTATGACTG ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT
            541 CTGTATCTGG CGGACCCGTG GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG
            601 GAGACGTCCC AGGGACTTCG GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG
            661 ATCGTTTTGG ACTCTTTGGT GCACCCCCCT TAGAGGANGG ATATGTGGTT CTGGTAGGAG
            721 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGGANCGAA
            781 NCCGCGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGANTGTGT
            841 TTCTGTATTT GTCTGAAAAT ATGGGCCCGG GNCAGANNGN TACCNCTCCC TTAAGTTTGA
            901 NNNNNNNTCN NTGGAAAGNN GTCNANNGGN TCGNTCNNNN NNNGNCNGNA NNNGNNNNNN
          961 ANNANNNNTN NGNNNANNNN NNGNNNNNCN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826.

          2023.04.06

          GNP_filter3_RDBDEP_html_230405.pl