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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-bsr-ss-EGFP (#RDB17363)

Retroviral expression vector of secretory form of EGFP.

Clone info. Retroviral expression vector of secretory form of EGFP, tagged with the signal sequence from mouse calreticulin (ss) at N-terminus.
Vector backbone pMRX-IRES-bsr (plasmid)
Selectable markers Amp^r (E. coli), Blasticidin^r (mammalian cells).
Gene/insert name mouse Calr cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17363 pMRX-bsr-ss-EGFP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMRX-bsr-ss-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17363).

Reference section:

Homma, Y., Kinoshita, R., Kuchitsu, Y., Wawro, P.S., Marubashi, S., Oguchi, M.E., Ishida, M., Fujita, N., Fukuda, M., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17363_A9Lgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region, SS, EGFP 5'
Sequence file: RDB17363_A9Lga.seq check
>D06377A7_A9Lg_2_MMLV-pol_F_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNGGNAT CGCAGCTTGG NNNCNCGCCG CCCNCGTGAA GGCTGCCGAC
   61 CCCGGGGGTG GACCATCCTC TAGACTGCCG GATCTGCCAC CATGCTCCTT TCGGTGCCGC
  121 TCCTGCTTGG CCTCCTCGGC CTGGCCGCCG CAAGATCCAT GGTGAGCAAG GGCGAGGAGC
  181 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT
  241 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA
  301 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG
  361 GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG
  421 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA
  481 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG
  541 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA
  601 GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA
  661 TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC
  721 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC
  781 TGANCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGANTTC GTGACCGCCG
  841 CCGGNNTCAC TCTCGGCATG GACGANCTGN ACAAGGGNTC CNNANCGGNC GCGGNAACAA
  901 TTGTTNACTN NNTNNNGCTA GCNACNGTTT CCNNCTANCG GGNNCNATTN CGCCCCCCCC
961 NNCCNANNNN NANNGNNNGN NNNCNNNNNN NNNN
//
Primer: IRES-R (Pr0037)
Region: EGFP 3'
Sequence file: RDB17363_A9Lgb.seq check
>D06377A7_A9Lg_2_IRES-R_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTTA GGGGGGGGGG GCGGNNNTGN TCCCGCNAGA
   61 GGGAAACCGT TGCTAGCTTA AGTTAGTTAA CAATTGTTAC CGCGGCCGCT TAGGATCCCT
  121 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA
  181 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT
  241 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
  301 GCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT
  361 TCTGCTTGTC GGCCATGATA TANACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC
  421 CCAGGATGTT GCCGTCCTCC TTGAAGTCNA TGCCCTTCAG CTCGATGCGG TTCACCAGGG
  481 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAANAANA
  541 TGGTGCGCTC CTGGACGTAN CCTTCGGGCA TGGCGGACTT GAANAAGTCN TGCTGCTTCA
  601 TGTGGTCGGG GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG
  661 GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC ANATGAACTT CAGGGTCAGC TTGCCGTAGG
  721 TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA
  781 GCTCGACCAG GANGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGATC
  841 TTGCGGCGGC CANGCCGANG NNGNCNAGCA GGNNCGGCNC CNAAAGGANC ATGGNGNNAN
  901 ATNCGGNANN CTANANGNNG GNCCACCCCC NGGGNCGGCA GCNNTNNCGT NNNNGNGNGN
961 ATNCNAGCTG CNATNNCGNN TANTTTNANG NNNNNGGGGG TNNNNANNNN NNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES, bsr, 3' LTR
Sequence file: RDB17363_A9Lgc.seq check
>D06377A7_A9Lg_2_IRES-F_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGT CGAGGTNNNN NNNNNNTCTA GGCCCCCCGA ACCACGGGGN
   61 CGTGGTTTTC CTTTGAAAAA CACGATAATA CCATGGTCAT GAAAACATTT AACATTTCTC
  121 AACAAGATCT AGAATTAGTA GAAGTAGCGA CAGAGAAGAT TACAATGCTT TATGAGGATA
  181 ATAAACATCA TGTGGGAGCG GCAATTCGTA CGAAAACAGG AGAAATCATT TCGGCAGTAC
  241 ATATTGAAGC GTATATAGGA CGAGTAACTG TTTGTGCAGA AGCCATTGCG ATTGGTAGTG
  301 CAGTTTCGAA TGGACAAAAG GATTTTGACA CGATTGTAGC TGTTAGACAC CCTTATTCTG
  361 ACGAAGTAGA TAGAAGTATT CGAGTGGTAA GTCCTTGTGG TATGTGTAGG GAGTTGATTT
  421 CAGACTATGC ACCAGATTGT TTTGTGTTAA TAGAAATGAA TGGCAAGTTA GTCAAAACTA
  481 CGATTGAAGA ACTCATTCCA CTCAAATATA CCCGAAATTA AAAGTCGACG ATAAAATAAA
  541 AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA
  601 AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAGA
  661 AGTTCAGATC AAGGTCAGGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG
  721 TGGTAAGCAG TTCCTGCCCC GGCTCANGGC CNAGAACAGA TGGAACAGCT GAATATGGGC
  781 CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC ANGGCCNAGA ACAGATGGTC
  841 CCCAGATGCG GTCCAGCCCT CAGCAGTTTC TAGAGAANCA TCANANGTTT CCNGGGNGNC
  901 CCAAGGANCT GAANTGANCC TGNGCCTTAT TTGAACTNAC CNATCAGTTC GCTTCNCGCT
  961 NCNNTTCNNN NGCTTCNGNT NCNNNANCNC NNNAAAAGAN NNNNNNNNCN NNNNNNNNGG
1021 NNNNNNNNNN
//
Primer: CMV enh_F1 (Pr0652)
Region: CMV en, 5' LTR, MMLV psi
Sequence file: RDB17363_A9Lgd.seq check
>D06377A7_A9Lg_2_CMVenh_F1_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN NNNNNNNTGN NCTTATGGGA NTTTCCTACT TGGCAGNANN
   61 NCTACGTATT AGTCATCGCT ATTACCATGC TAGAGAACCA TCAGATGTTT CCAGGGTGCC
  121 CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT
  181 TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC
  241 GCGCCAGTCC TCCGATTGAC TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG
  301 CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC
  361 TACCCGTCAG CGGGGGTCTT TCATTTGGGG GCTCGTCCGG GATCGGGAGA CCCCTGCCCA
  421 GGGACCACCG ACCCACCACC GGGAGGTAAG CTGGCCAGCA ACTTATCTGT GTCTGTCCGA
  481 TTGTCTAGTG TCTATGACTG ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT
  541 CTGTATCTGG CGGACCCGTG GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG
  601 GAGACGTCCC AGGGACTTCG GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG
  661 ATCGTTTTGG ACTCTTTGGT GCACCCCCCT TAGAGGANGG ATATGTGGTT CTGGTAGGAG
  721 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGGANCGAA
  781 NCCGCGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGANTGTGT
  841 TTCTGTATTT GTCTGAAAAT ATGGGCCCGG GNCAGANNGN TACCNCTCCC TTAAGTTTGA
  901 NNNNNNNTCN NTGGAAAGNN GTCNANNGGN TCGNTCNNNN NNNGNCNGNA NNNGNNNNNN
961 ANNANNNNTN NGNNNANNNN NNGNNNNNCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826. [link to RRC of NBRP]