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pEGFP-C1-Rab12-T55N (#RDB16881)

Expression vector of mouse Rab12A constitutive negative mutant, T55N, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab12A constitutive negative mutant, T55N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab12 cDNA
Reference of insert sequence AB232607 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16881 pEGFP-C1-Rab12-T55N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab12-T55N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16881).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16881_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB16881_A9A8a.seq check
>D06060G1_A9A8_2_EGFP-C2_B05_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNCNCGN NCNCNTGGTC CTGCTGGANT TCGTGNCCGC CGCCGGGATC
   61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTATGGATCC CAGCGCCGCG
  121 CTGCACCGAC GGCCCGCGGG CGGCAGCCTG GGCGCGGTCT CCCCGGCGCT GTCGGGCGGC
  181 CAGGCCCGCC GGAGGAAGCA GCCCCCCAGG CCGGCCGACT TCAAGCTGCA GGTCATCATC
  241 ATCGGCTCCC GCGGCGTGGG CAAGAACAGC CTGATGGAGC GCTTCACCGA CGACACGTTC
  301 TGCGAGGCCT GCAAGTCCAC CGTGGGTGTT GACTTTAAAA TCAAGACTGT AGAACTAAGA
  361 GGGAAGAAAA TTAGATTACA GATCTGGGAC ACAGCAGGGC AGGAGCGATT TAACAGCATT
  421 ACCTCAGCCT ATTACAGAAG TGCCAAGGGG ATCATACTCG TATATGACAT CACCAAGAAG
  481 GAGACGTTCG ATGACTTGCC AAAGTGGATG AAGATGATTG ACAAGTATGC TTCAGAAGAC
  541 GCCGAGCTCC TCCTGGTTGG AAATAAGCTG GACTGTGAGA CAGACAGAGA AATCTCAAGG
  601 CAGCAAGGAG AAAAGTTTGC ACAGCAGATA ACCGGGATGC GCTTCTGCGA AGCCAGTGCC
  661 AAGGACAATT TCAATGTGGA CGAGATCTTT CTGAAACTCG TTGATGATAT TCTGAAAAAG
  721 ATGCCTCTTG ATGTTTTGAG AAACGAGTTA TCCAATAGCA TCCTTTCTCT ACNACCAGAG
  781 CCTGANATCC CACCAGANCT GCCTCCTCCC AGANCACACG TCCNATGCTG NTGATTTGAT
  841 ACTTTGGAGA ATCACTANTG AATTCTGCAG NCGANNGTAN CGCGGGNCCN GGNTNCNNCG
  901 GATCTAGATA NNTGATCATA ATCAGCNNTA CNNNATTTGT ANANGNTTTN CTTGCTTTAA
  961 AAANNNNCCA CANNNCCCCN NGAANCNGAA ANATAAAATG NNNGNNNNNG NNGNNGNTNN
 1021 CNTNGNTTNN NNNANCNNAN NANNNNNNNN ANNNNNNANN NCNNNNNNNN TNNNNNNNNN
1081 N
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB16881_A9A8b.seq check
>D06060G1_A9A8_2_SV40polyA-R2_C05_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNANN NAANNANGNN NNNNNNAACA ATTGCATTCN TTTTATGTTT
   61 CNGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
  121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC
  181 TGCAGAATTC ACTAGTGATT CTCCAAAGTA TCAAATCAAC AGCATCGGAC GTGTGGTCTG
  241 GGAGGAGGCA GCTCTGGTGG GATCTCAGGC TCTGGTTGTA GAGAAAGGAT GCTATTGGAT
  301 AACTCGTTTC TCAAAACATC AAGAGGCATC TTTTTCAGAA TATCATCAAC GAGTTTCAGA
  361 AAGATCTCGT CCACATTGAA ATTGTCCTTG GCACTGGCTT CGCAGAAGCG CATCCCGGTT
  421 ATCTGCTGTG CAAACTTTTC TCCTTGCTGC CTTGAGATTT CTCTGTCTGT CTCACAGTCC
  481 AGCTTATTTC CAACCAGGAG GAGCTCGGCG TCTTCTGAAG CATACTTGTC AATCATCTTC
  541 ATCCACTTTG GCAAGTCATC GAACGTCTCC TTCTTGGTGA TGTCATATAC GAGTATGATC
  601 CCCTTGGCAC TTCTGTAATA GGCTGAGGTA ATGCTGTTAA ATCGCTCCTG CCCTGCTGTG
  661 TCCCAGATCT GTAATCTAAT TTTCTTCCCT CTTAGTTCTA CAGTCTTGAT TTTAAAGTCA
  721 ACACCCACGG TGGACTTGCA GGCCTCGCAG AACGTGTCGT CNGTGAAGCG CTCCATCAGG
  781 CTGTTCTTGC CCACGCCGCG GGANCCGANG ATGATGANCT GCAGCTTGAA GTCGGNCGGN
  841 CTGGGGGGNT GCTTCCTCNN NNGGGCCTGG NCGCCCGANA GCGCCNGGGA GANCGCGCCC
  901 AGGCTGNCGC CCGCGGGNCC NTCGGTGCAN CGCGGCGCTG GGANNNNTAN NTCTGANNCC
  961 NGANTTGNAN AGCNNGTCCA TGCCNANNNT GATNCNNNCG GNNGNCACNA ACNNCANCAN
1021 NNNCNNGTGN TNNCNCNTNN NGNNGGGGNN TTNNNNCNNN NNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB16881_A9A8c.seq check
>D06060G1_A9A8_2_CMV_Forward_D05_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTGNNNCAN NNGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
  601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
  661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
  721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
  781 TGCTGGAGTT CGTGACCGCC GCNNGGATCA CTCTCGGCAT GGNNGAGCTG TACAAGTCCG
  841 GACTCAGATC TATGGATCCC AGCGCCGCGC TGCACCGNCN GNCCGCGGGC GGNANCCNNN
  901 NCGCGGTCTC CCNGGCNCTG NCNGGNGGNC AGGCCCGCNN GNNGAANCAN CCCCCCCNNG
  961 CNGGCCNANN NCNAGCTGNN NGNNNNTCNT CNTNGNNTCC NNNNNCGNGG NCAANAANNN
1021 NNCNNGANNG NANCGNNNNN NNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16881_A9A8d.seq check
>D06060G1_A9A8_2_SV40pro_F_V2_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CTTTTNNNNN NNCGATCAAG AGANAGGNTG AGGNTCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAG
  721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGANCGC
  781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  841 CTTGACGANT TCTTCTGANC GGGANTCTGG GGTTCGAAAT GACNGANCAA GCGACGCCCA
  901 ACCTGCCATC ACGAGATTTC GANNCCACCG CCGCCTTCTA TGNNNNGNNG GGCTTCGGAN
  961 TCGTTTTCCN GNNNGNCNGC TNNNTNNNNC NTNNNGCGCG GGGNTNNCNN GCNNNNNNNN
1021 NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]