RIKEN DNA Bank Mouse Resource

RIKEN DNA Bank Mouse Resource - Rab12

Gene ID	NCBI Gene 19328 \| KEGG mmu:19328
Gene Symbol	Rab12
Protein Name	RAB12, member RAS oncogene family
Synonyms	2900054P15Rik
Featured content	Rab Family - mouse

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Ortholog resource in our bank

Rab12

External dsatabase

mouse Rab12

Individualy Deposited Resource

Catalog number	Name of Resource	Description	CDS comparison
Catalog number	Name of Resource	Description	Refered (NCBI mRNA)	CDS status⁽¹⁾
RDB20739	pMRX-puro-mStr-Rab12-N154I	Expression vector of mouse mStrawberry-Rab12 constitutive negative mutant (N154I).
RDB18853	pGBD-C1-Rab12-deltaCys-N154I	Expression vector of mouse Rab12 constitutive negative mutant (N154I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
RDB18852	pGBD-C1-Rab12-deltaCys-Q100L	Expression vector of mouse Rab12 constitutive active mutant (Q100L) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
RDB17408	pGBD-C1-Rab12-delta Cys-T55N	Expression vector of constitutive negative mutants of mouse Rab12 (T55N) for yeast two-hybrid assay.
RDB17259	pEGFP-C1-Rab12	Expression vector of mouse Rab12 fused with EGFP.
RDB16882	pEGFP-C1-Rab12-Q100L	Expression vector of mouse Rab12A constitutive active mutant, Q100L, fused with EGFP at N-terminus.
RDB16881	pEGFP-C1-Rab12-T55N	Expression vector of mouse Rab12A constitutive negative mutant, T55N, fused with EGFP at N-terminus.
RDB16837	pGEX-4T-3-Rab12	Expression vector of mouse Rab12 tagged with GST at N-terminus, tac promoter.
RDB15393	pEF-FLAG-mouse Rab12	Expression vector of mouse RAB12, N-terminal FLAG-tag.

(1) CDS status was determined by comparing the plasmid sequence with NCBI RefSeq mRNA.

webcatalog20240727.tab

NIA/NIH Mouse cDNA Clone

Plasmid request [in Japanese] [in English]

Catalog number	Clone name	Vector	Sequence		Status
Catalog number	Clone name	Vector	5' terminal EST, submitted in DDBJ⁽¹⁾	3' terminal EST, submitted in DDBJ⁽¹⁾	Status
MCH057307	H3143E11	pSPORT1	BG087596	BG075124

♦ Full length sequence is not available. The clone could differ from the NCBI mRNA reference sequence.
(1) Actual EST sequence either 5' or 3' terminal end. Data were submitted to the DNA Data Bank of Japan (DDBJ).
♦ NIA/NIH Mouse cDNA clone was deposited by Dr Minoru Ko (Tanaka et al., PNAS 97, 9127-9132, 2000. VanBuren V, et al. Genome Research 12, 1999-2003, 2002) at the National Institute on Aging, the National Institutes of Health, U.S.A.

NIAmouse22k_2024May11.csv

Mouse BAC clone

Clone request [in Japanese] [in English]

BAC clone search	[map around Gene ID 19328] (link to our BAC browser)

♦ Every single our BAC clones have analyzed their terminal end sequences to map on the genome. Most of them are assigned by both ends and indicated with blue line on the genome browser (they likely contain the region). Others are assigned only by a single end and indicated with red line on the genome browser (containing piece is unsure).
♦ Reasons that we did not use the other end result for mapping are: (1) Data was not suitable for BLAST analysis (short read, repeats, no data or such stuff); (2) Result of BLAST analysis is unlikely [two ends are, too far away (>0.3 Mb), distinct chromosome, same orientation, or such incomprehensible].
♦ Full length sequence is not available.

2025.03.12

Mus_musculus_gene_info230514.csv - RDB_mmu_GIxxxxxxxxx_html_240727.pl