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pEGFP-C1-Rab5A-S34N (#RDB16874)

Expression vector of mouse Rab5A constitutive negative mutant, S34N, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab5A constitutive negative mutant, S34N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab5a cDNA
Reference of insert sequence AB232593 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16874 pEGFP-C1-Rab5A-S34N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab5A-S34N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16874).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16874_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP, insert 5'
Sequence file: RDB16874_A9A8a.seq check
>D06060F3_A9A8_2_EGFP-C2_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNCNCGAT CNCNTGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
   61 CTCTCGGCAT GGNCGAGCTG TACAAGTCCG GACTCAGATC CATGGCTAAT CGAGGAGCAA
  121 CAAGACCCAA CGGGCCAAAT ACTGGAAATA AAATATGCCA GTTCAAACTG GTCCTTCTAG
  181 GAGAGTCCGC GGTTGGCAAA AACAGCCTGG TTCTTCGCTT TGTGAAAGGC CAATTTCATG
  241 AATTTCAAGA GAGTACCATT GGGGCTGCCT TTCTAACCCA AACTGTGTGT CTTGATGACA
  301 CAACAGTAAA ATTTGAAATA TGGGATACAG CTGGTCAAGA ACGGTATCAT AGCTTAGCAC
  361 CAATGTACTA CCGAGGAGCA CAAGCAGCCA TAGTTGTGTA TGATATCACA AATGAGGAAT
  421 CCTTTGCGAG AGCAAAAAAC TGGGTTAAAG AACTTCAAAG GCAAGCAAGT CCTAATATTG
  481 TGATAGCTTT GTCAGGAAAC AAAGCTGACT TAGCAAATAA AAGAGCTGTT GACTTCCAGG
  541 AAGCACAGTC CTATGCAGAT GACAACAGCT TATTATTTAT GGAGACATCA GCTAAGACAT
  601 CAATGAATGT AAATGAAATA TTTATGGCAA TAGCTAAAAA GCTGCCAAAG AATGAACCAC
  661 AGAATCCTGG TGCAAACTCA GCCAGAGGAC GANGAGTAGA CCTTACTGAG CCTGCACAGC
  721 CAGCCAGAAG CCAGTGTTGT AGTAACTGAA ATCGAATTCG ATATCAAGCT TATCGATACC
  781 GTCGACNGTA CCGCGGGCCC GGGNNCCACC GGATCTAGAT AACTGATCAT AATCAGCCNT
  841 ACCACATTTG TAGAGGNTTN ACTTGCTTTN AAAANNCTNC CNCACCNCCC CCNGAANCNG
  901 AAACNTNAAA TGNANGCNAT TGNTGNTGTT NNCNTGNTTA TTGNNNCTTA NNNNGGNTAN
961 NAATNANGCN NNANCATCAN NAATTTNNCA AATAAAGNNN TTTTTTNNNN NNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB16874_A9A8b.seq check
>D06060F3_A9A8_2_SV40polyA-R2_F04_16_ABI24.ab1
    1 NNNNNNNNNC NNNNNNANNN NGTTAANNAN AACNATTGCA TTCNTTTTAT GTTTNNGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACGGTATC
  181 GATAAGCTTG ATATCGAATT CGATTTCAGT TACTACAACA CTGGCTTCTG GCTGGCTGTG
  241 CAGGCTCAGT AAGGTCTACT CCTCGTCCTC TGGCTGAGTT TGCACCAGGA TTCTGTGGTT
  301 CATTCTTTGG CAGCTTTTTA GCTATTGCCA TAAATATTTC ATTTACATTC ATTGATGTCT
  361 TAGCTGATGT CTCCATAAAT AATAAGCTGT TGTCATCTGC ATAGGACTGT GCTTCCTGGA
  421 AGTCAACAGC TCTTTTATTT GCTAAGTCAG CTTTGTTTCC TGACAAAGCT ATCACAATAT
  481 TAGGACTTGC TTGCCTTTGA AGTTCTTTAA CCCAGTTTTT TGCTCTCGCA AAGGATTCCT
  541 CATTTGTGAT ATCATACACA ACTATGGCTG CTTGTGCTCC TCGGTAGTAC ATTGGTGCTA
  601 AGCTATGATA CCGTTCTTGA CCAGCTGTAT CCCATATTTC AAATTTTACT GTTGTGTCAT
  661 CAAGACACAC AGTTTGGGTT AGAAAGGCAG CCCCAATGGT ACTCTCTTGA AATTCATGAA
  721 ATTGGCCTTT CACAAAGCGA AGAACCAGGC TGTTTTTGCC NACCGCGGAC TCTCCTAGAA
  781 GGANCAGTTT GAACTGGCAT ATTTTATTTC CAGTATTTGG CCCGTTGGGT CNTGTTGCTC
  841 CTCGATTANC CATGGNTCTG ANNNCGGANT TGTACAGCTC GTCCATGCCG ANANTGATNC
  901 NGGCGGCGGN CACGAACTCC ANCNNNANCN TGTGNNNGCN CTTCTCGTTG GGGNCNTTGN
  961 NCNNNNNNAN NNGNNGNTCN NNNANNNGNN NNNNNGGNAN CANCNNGGGN NNNNCNNNNN
1021 NNGGGGNNNN NNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP
Sequence file: RDB16874_A9A8c.seq check
>D06060F3_A9A8_2_CMV_Forward_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
  121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
  421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
  601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
  661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN
  721 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
  781 GTTCGTGACC GCCGCCGGGN TCACTCTCGG CATGGNCGAN CTGTACAAGT CCGGANTCNN
  841 ATCCATGGCT AATCGAGGAN CAACAAGANC CAACGGGCCN AATACTGNAA ATAAAATATG
  901 NCAGTTCNAA CTGGNCCTTC NNGGANANNC CGNGGNNGGN AAAAACAGCC NNNNTNNNNC
961 GCTTTGNGNA ANNNNNNNNT TCNTGNNNTN NNNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16874_A9A8d.seq check
>D06060F3_A9A8_2_SV40pro_F_V2_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGCTTTTGCA AGNNCGATCN AGAGNNNGGN NGAGGNNCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGANCT TGGCGGCGAA TGGGCTGANC
  781 GCTTCCTCGT GCTTTACNGT ATCGCCGCTC NCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACNA NTTCTTCTGA NCGGGANTCT NGGGTTCGAA ATGNNNNANN NANCGACGCC
  901 NNACCNGCCN TCACNANANT TCGANNNCNN CNCCNNNNTC TATNAAANGN NNNGNNTTNN
961 GNNNCGNTTT TNNNGGANGC NNNCNNGNNN NNNNCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]