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pSilencer-neo-Rab5A

Expression vector of shRNA against mouse Rab5A.

Catalog number RDB15727
Resource name pSilencer-neo-Rab5A
Clone info. Expression vector of shRNA against mouse Rab5A (19-base target site 5'-GCACAGTCCTATGCAGATG-3'). U6 promoter.
Expression was confirmed by the depositor with confocal microscopy. Please refer Mori, Y. et al., J. Biol. Chem., 288 (14): 9835-9847, 2013 (PMID 23430262) for gene cloning.
Vector backbone pSilencer 2.1-U6 neo (plasmid)
Size of vector backbone 4.5 kb
Selectable markers Amp^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab5a cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab5a (NCBI Gene 271457) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mori, Y. et al. J. Biol. Chem. 288, 9835-9847, 2013); (Azouz, N. P. et al. J. Immunol. 192, 4043-4053, 2014)
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RDB15727 pSilencer-neo-Rab5A DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Azouz, N.P., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15727_A8A9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13fwd RDB15727_A8A9a.seq
>D05154A4_1_A8A9_M13fwd_A02_02_ABI24.ab1
    1 CGGACGGCGC AGGCAACGCA CCACGTGACG GAGCGTGACC GCGCGCCGAG CGCGCGCCAA
   61 GGTCGGGCAG GAAGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA
  121 GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA
  181 TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA
  241 AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG GTTTATATAT
  301 CTTGTGGAAA GGACGCGGGA TCCGCACAGT CCTATGCAGA TGTTCAAGAG ACATCTGCAT
  361 AGGACTGTGC TTTTTTGGAA AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
  421 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC
  481 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
  541 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
  601 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT
  661 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
  721 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
  781 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
  841 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
  901 CCTGGAAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GGATACCTGT
  961 CCGCCTTTCT CCCTTTCGGA GCGTGGCGCT TTTCTCATAG CTCACGGCTG TAGTATCTCA
 1021 GTTCGGTGTA AGTTCGGTCG CTCTAGCTGG CTGTGTGCAC GACCCCCCGA TCACCCGACG
1081 CTGCGCTTAT CAGTACCTAT CGATCCTTGG AGATC
//
Primer: SV40pro_F RDB15727_A8A9b.seq
>D05154A4_1_A8A9_SV40pro_F_C02_08_ABI24.ab1
    1 CCTTGGGTTC CCGGGCATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG
  121 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
  181 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
  241 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
  301 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
  361 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
  421 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
  481 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
  541 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
  601 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC
  661 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
  721 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
  781 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
  841 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCCTTC
  901 TATCGCCTTT CTTGACGAGT TCTTTCTGAG CGGGACTCTG GGGGTTCGCG AAATGACCGA
  961 CCAAGCGACG GCCCAACCTG CCATCACGAG ATTTCGATTT CAACGCGCCT TTCTATGAAA
 1021 GGTTGGGGCT CGGAATCGTT TCCGGACGTG GCTGGATGAT CTTCAGCGCG GGGAATCTCA
1081 TGCTTGGAGT CCTTTTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Azouz, N.P., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234.
reference Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262.

2019.05.16

GNP_filter3_RDBDEP_html_190430.pl