Expression vector of shRNA against mouse Rab5A.
Clone info. | Expression vector of shRNA against mouse Rab5A (19-base target site 5'-GCACAGTCCTATGCAGATG-3'). U6 promoter. |
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Comment | Expression was confirmed by the depositor with confocal microscopy. Please refer Mori, Y. et al., J. Biol. Chem., 288 (14): 9835-9847, 2013 (PMID 23430262) for gene cloning. |
Vector backbone | pSilencer 2.1-U6 neo (plasmid) |
Size of vector backbone | 4.5 kb |
Selectable markers | Amp^r (E. coli), Neo^R (mammalian cells). |
Gene/insert name | mouse Rab5a cDNA |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mori, Y. et al. J. Biol. Chem. 288, 9835-9847, 2013); (Azouz, N. P. et al. J. Immunol. 192, 4043-4053, 2014) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. J. Biol. Chem. 288, 9835-9847, 2013); (Azouz, N. P. et al. J. Immunol. 192, 4043-4053, 2014)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15727 | pSilencer-neo-Rab5A | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pSilencer-neo-Rab5A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15727). |
Reference section:
Azouz, N.P., Zur, N., Efergan, A., Ohbayashi, N., Fukuda, M., Amihai, D., Hammel, I., Rothenberg, M.E., Sagi-Eisenberg, R., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15727_A8A9p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13fwd () Sequence file: RDB15727_A8A9a.seq ![]() |
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>D05154A4_1_A8A9_M13fwd_A02_02_ABI24.ab1 1 CGGACGGCGC AGGCAACGCA CCACGTGACG GAGCGTGACC GCGCGCCGAG CGCGCGCCAA 61 GGTCGGGCAG GAAGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA 121 GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA 181 TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA 241 AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG GTTTATATAT 301 CTTGTGGAAA GGACGCGGGA TCCGCACAGT CCTATGCAGA TGTTCAAGAG ACATCTGCAT 361 AGGACTGTGC TTTTTTGGAA AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA 421 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC 481 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC 541 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC 601 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT 661 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 721 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 781 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 841 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 901 CCTGGAAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GGATACCTGT 961 CCGCCTTTCT CCCTTTCGGA GCGTGGCGCT TTTCTCATAG CTCACGGCTG TAGTATCTCA 1021 GTTCGGTGTA AGTTCGGTCG CTCTAGCTGG CTGTGTGCAC GACCCCCCGA TCACCCGACG 1081 CTGCGCTTAT CAGTACCTAT CGATCCTTGG AGATC // |
Primer: SV40pro_F () Sequence file: RDB15727_A8A9b.seq ![]() |
>D05154A4_1_A8A9_SV40pro_F_C02_08_ABI24.ab1 1 CCTTGGGTTC CCGGGCATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT 61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG 121 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG 181 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT 241 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC 301 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT 361 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG 421 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG 481 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG 541 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG 601 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC 661 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA 721 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC 781 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG 841 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCCTTC 901 TATCGCCTTT CTTGACGAGT TCTTTCTGAG CGGGACTCTG GGGGTTCGCG AAATGACCGA 961 CCAAGCGACG GCCCAACCTG CCATCACGAG ATTTCGATTT CAACGCGCCT TTCTATGAAA 1021 GGTTGGGGCT CGGAATCGTT TCCGGACGTG GCTGGATGAT CTTCAGCGCG GGGAATCTCA 1081 TGCTTGGAGT CCTTTTC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Azouz, N.P., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234. [link to RRC of NBRP] |
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reference | Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262. [link to RRC of NBRP] |
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