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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSilencer-neo-Rab5A (#RDB15727)

Expression vector of shRNA against mouse Rab5A.

Clone info. Expression vector of shRNA against mouse Rab5A (19-base target site 5'-GCACAGTCCTATGCAGATG-3'). U6 promoter.
Comment Expression was confirmed by the depositor with confocal microscopy. Please refer Mori, Y. et al., J. Biol. Chem., 288 (14): 9835-9847, 2013 (PMID 23430262) for gene cloning.
Vector backbone pSilencer 2.1-U6 neo (plasmid)
Size of vector backbone 4.5 kb
Selectable markers Amp^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab5a cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mori, Y. et al. J. Biol. Chem. 288, 9835-9847, 2013); (Azouz, N. P. et al. J. Immunol. 192, 4043-4053, 2014)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. J. Biol. Chem. 288, 9835-9847, 2013); (Azouz, N. P. et al. J. Immunol. 192, 4043-4053, 2014)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15727 pSilencer-neo-Rab5A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSilencer-neo-Rab5A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15727).

Reference section:

Azouz, N.P., Zur, N., Efergan, A., Ohbayashi, N., Fukuda, M., Amihai, D., Hammel, I., Rothenberg, M.E., Sagi-Eisenberg, R., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15727_A8A9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13fwd ()
Sequence file: RDB15727_A8A9a.seq check
>D05154A4_1_A8A9_M13fwd_A02_02_ABI24.ab1
    1 CGGACGGCGC AGGCAACGCA CCACGTGACG GAGCGTGACC GCGCGCCGAG CGCGCGCCAA
   61 GGTCGGGCAG GAAGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA
  121 GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA
  181 TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA
  241 AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG GTTTATATAT
  301 CTTGTGGAAA GGACGCGGGA TCCGCACAGT CCTATGCAGA TGTTCAAGAG ACATCTGCAT
  361 AGGACTGTGC TTTTTTGGAA AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
  421 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC
  481 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
  541 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
  601 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT
  661 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
  721 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
  781 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
  841 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
  901 CCTGGAAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GGATACCTGT
  961 CCGCCTTTCT CCCTTTCGGA GCGTGGCGCT TTTCTCATAG CTCACGGCTG TAGTATCTCA
 1021 GTTCGGTGTA AGTTCGGTCG CTCTAGCTGG CTGTGTGCAC GACCCCCCGA TCACCCGACG
1081 CTGCGCTTAT CAGTACCTAT CGATCCTTGG AGATC
//
Primer: SV40pro_F ()
Sequence file: RDB15727_A8A9b.seq check
>D05154A4_1_A8A9_SV40pro_F_C02_08_ABI24.ab1
    1 CCTTGGGTTC CCGGGCATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG
  121 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
  181 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
  241 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
  301 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
  361 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
  421 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
  481 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
  541 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
  601 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC
  661 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
  721 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
  781 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
  841 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCCTTC
  901 TATCGCCTTT CTTGACGAGT TCTTTCTGAG CGGGACTCTG GGGGTTCGCG AAATGACCGA
  961 CCAAGCGACG GCCCAACCTG CCATCACGAG ATTTCGATTT CAACGCGCCT TTCTATGAAA
 1021 GGTTGGGGCT CGGAATCGTT TCCGGACGTG GCTGGATGAT CTTCAGCGCG GGGAATCTCA
1081 TGCTTGGAGT CCTTTTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Azouz, N.P., Rab5 is a novel regulator of mast cell secretory granules: impact on size, cargo, and exocytosis. J. Immunol. 192 (9): 4043-4053 (2014). PMID 24696234. [link to RRC of NBRP]
reference Mori, Y., Rabex-5 protein regulates dendritic localization of small GTPase Rab17 and neurite morphogenesis in hippocampal neurons. J. Biol. Chem. 288 (14): 9835-9847 (2013). PMID 23430262. [link to RRC of NBRP]