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pEGFP-C1-mouse Rab5A-Q79L (#RDB15726)

Expression vector of mouse Rab5A constitutive active mutant EGFP-tag.

Clone info. Expression vector of mouse Rab5A constitutive active mutant (GTP-hydrolysis-deficient, Q79L), fused with EGFP at N-terminal. CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope. Please refer Itoh, T. et al., Genes. Cells, 11 (9): 1023-1037, 2006 (PMID 16923123) for gene cloning.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab5a cDNA
Depositor|Developer Fukuda, Mitsunori |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)。

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RDB15726 pEGFP-C1-mouse Rab5A-Q79L DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Rab5A-Q79L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15726).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15726_A8A9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP, insert 5'
Sequence file: RDB15726_A8A9a.seq check
>D05154A3_A8A9_1_EGFP-C2_E10_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNCGNNC NCNTGGTCCT GCTGGANTTC GTGACCGCCG CCGGGNTCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCTAATC GAGGAGCAAC
  121 AAGACCCAAC GGGCCAAATA CTGGAAATAA AATATGCCAG TTCAAACTGG TCCTTCTAGG
  181 AGAGTCTGCT GTTGGCAAAT CAAGCCTGGT TCTTCGCTTT GTGAAAGGCC AATTTCATGA
  241 ATTTCAAGAG AGTACCATTG GGGCTGCCTT TCTAACCCAA ACTGTGTGTC TTGATGACAC
  301 AACAGTAAAA TTTGAAATAT GGGATACAGC TGGTCTCGAG CGGTATCATA GCTTAGCACC
  361 AATGTACTAC CGAGGAGCAC AAGCAGCCAT AGTTGTGTAT GATATCACAA ATGAGGAATC
  421 CTTTGCGAGA GCAAAAAACT GGGTTAAAGA ACTTCAAAGG CAAGCAAGTC CTAATATTGT
  481 GATAGCTTTG TCAGGAAACA AAGCTGACTT AGCAAATAAA AGAGCTGTTG ACTTCCAGGA
  541 AGCACAGTCC TATGCAGATG ACAACAGCTT ATTATTTATG GAGACATCAG CTAAGACATC
  601 AATGAATGTA AATGAAATAT TTATGGCAAT AGCTAAAAAG CTGCCAAAGA ATGAACCACA
  661 GAATCCTGGT GCAAACTCAG CCAGAGGANG AGGAGTAGAC CTTACTGAGC CTGCACAGCC
  721 AGCCAGAAGC CAGTGTTGTA GTAACTGAAA TCGAATAATC GAATTCTGCA GTCGACGGTA
  781 CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAT AATCNGCCNT ACCACATTTG
  841 TAGANGNTTT ACTTGCTTTN AAAAACCTCC CNNACCNNCC CCNNAANCTG AAANATNAAA
  901 TGAANGNNAT TGNNGNTGNT NNCTTGNTTA NNNNANCNTN NTAANGGNNN NNANTAAAGC
961 NNNANCNTCN NNANNTTNNN NANNNANNNN NTTTTTTNNN NNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB15726_A8A9b.seq check
>D05154A3_A8A9_1_SV40polyA-R2_F10_16_ABI24.ab1
    1 NNNNNNNNNN NCNNNNNNNN NNNGTTAACA ANNACAATTG CATTCNTTTT ATGTTTCAGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
  181 GAATTCGATT ATTCGATTTC AGTTACTACA ACACTGGCTT CTGGCTGGCT GTGCAGGCTC
  241 AGTAAGGTCT ACTCCTCGTC CTCTGGCTGA GTTTGCACCA GGATTCTGTG GTTCATTCTT
  301 TGGCAGCTTT TTAGCTATTG CCATAAATAT TTCATTTACA TTCATTGATG TCTTAGCTGA
  361 TGTCTCCATA AATAATAAGC TGTTGTCATC TGCATAGGAC TGTGCTTCCT GGAAGTCAAC
  421 AGCTCTTTTA TTTGCTAAGT CAGCTTTGTT TCCTGACAAA GCTATCACAA TATTAGGACT
  481 TGCTTGCCTT TGAAGTTCTT TAACCCAGTT TTTTGCTCTC GCAAAGGATT CCTCATTTGT
  541 GATATCATAC ACAACTATGG CTGCTTGTGC TCCTCGGTAG TACATTGGTG CTAAGCTATG
  601 ATACCGCTCG AGACCAGCTG TATCCCATAT TTCAAATTTT ACTGTTGTGT CATCAAGACA
  661 CACAGTTTGG GTTAGAAAGG CAGCCCCAAT GGTACTCTCT TGAAATTCAT GAAATTGGCC
  721 TTTCACAAAG CGAAGAACCA GGCTTGATTT GCCAACAGCA GACTCTCCTA GAANGANCAG
  781 TTTGAACTGG CATATTTTAT TTCCAGTATT TGGCCCGTTG GGTCTTGTTG CTCCTCNATT
  841 ANCCATGGNT CTGANTCCGG ANTTGTACAG CTCGNCCATG NCGANANTGA TNCCGGCGNC
  901 GGNCACGANN TCCAGCAGGA NCATGTGATC GCGCTTNNCG TTGGGGNCTT TGCTNNNGNN
  961 GNANTGGGNG CTCNNNNANT GGNNNNCGGN NNNCANCNNG GGGNNNNNGN NNANGGGGGN
1021 NNNCNGNNNN NNNNNGNNNN NNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP
Sequence file: RDB15726_A8A9c.seq check
>D05154A3_1_A8A9_CMV-Forward_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNTNGNNG CNNTGGGCGG TANGCGTGTA CGGTGGGANG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACN ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCNA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGANCACTAC
  661 CAGCAGAACA CNNNCATCGG CGACGGCCCC GNGCTGCTGC CCGACAACCA CTACCTGANC
  721 ACCCAGTCCG CCCTGANCAA AGANCCCNAC GAGAANCGCG ATCACATNGN CCTGCTNGAN
781 TNCGTGANCG CCNCCNGGNT CANTCTCNNN NNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB15726_A8A9d.seq check
>D05154A3_A9Kr_1_SV40pro_F_V2_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTTG NAAGNNCGNN NANGAGNNAG GNNGAGGNNC GTTTCGCATG
   61 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC
  121 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
  181 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA
  241 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC
  301 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT
  361 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
  421 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
  481 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG
  541 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC
  601 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC
  661 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA
  721 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC
  781 GTGCTTTACG GTATCGCCGC TCCNGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC
  841 GAGTNCTTCT GANCGGGNNT CTGGGGTTCN NAATGNNNNA NCNAGCGACG CCCNANNNNN
901 NNTCACNANN TTNCGANTCC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]