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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse myosin Va MC-tail (Melanocyte type)

Expression vector of mouse melanocyte-type myosin Va tail region (- exon B, + exon D and F).

Catalog number RDB15285
Resource name pEF-FLAG-mouse myosin Va MC-tail (Melanocyte type)
Clone info. Expression vector of mouse melanocyte (MC)-type myosin Va tail region (- exon B, + exon D and F), tagged with N-terminal FLAG, EF1 promoter. ｃDNA fragment 3161nt to 5989nt of NM_010864.2 is cloned.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : G to A substitution at 3481nt of NM_010864.2 (Gln, Synonym).
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo5a cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Myo5a
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002).
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15285 pEF-FLAG-mouse myosin Va MC-tail (Melanocyte type) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15285_A7Gop1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB15285_A7Goa.seq check
          >D04773A3_A7Go_1_EF1a-C_F_C07_07_ABI24.ab1
              1 CCAGTCATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
             61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
            121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCGCTCGCT CTGTGGAACG
            181 CTACAAGAAG CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA
            241 TGAGCAGAAT AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA
            301 CAACTCTGAG ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA
            361 AGCTAAGGTT GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA
            421 AGACCTGGAA CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA
            481 ACAGGAAACT GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA
            541 AAAGGAGACC CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA
            601 GAGGAAGTTA GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG
            661 GTATCAGAAC CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA
            721 AGAGATGACC CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA
            781 CAGCAGTAAT GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC
            841 ACCAAGAACA GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA
            901 GCTCCAGAAG CGTGTCACAG AGCTGGAAAC AGGAGAAGCA GCTGATGCAG GATGAGCTGG
            961 ACCGCAAGGG AGGGAGCAGG TGTTCCCGCA GCAAGGCAAA GGAAGAAGAA AGGGCCACAG
           1021 ATAGAGGAGC TGACTAGAGT ATGAGTCTTC TCAGCGTCAG GAACTGCAGT TCAGAAACCA
          1081 AAAAACTGCA AAAAACGGAG CC
          //
          Primer: hCGSF_R
          Sequence file: RDB15285_A7Gob.seq check
          >D04773A3_A7Go_1_hGCSF_R_C08_08_ABI24.ab1
              1 CTCACTGATC TCTCAGGGTC CAGGTGGGGA CCCTCACTCT AGAGCGGCCG CTATGGTCGA
             61 CGGTATCGAT AAGCTTGATA TCGAATTCGA TTCAGACCCG TGCGATGAAG CCCAGGCCCA
            121 GGCTGGCTGG GATCTGGATG GTTTCTAGGG CTAGGGAGGA TGGGTTAAAG GGAAAAGTGA
            181 CAGGAAAGAT GTGTTTTGCA TCCATGAGCA GCTGTGGAGA GTCTTTCCTG TCTCGTAACC
            241 GCATCTGTAT AGTGCGGATA AATGAAACTG AGACCCTTTC TTCAAACTCA TTAACCGGTG
            301 TGTACAGATT CAACACTTTG ACAATCTGGG CCGTGGTCAG GGCGTTGCAC ATGGAACAGA
            361 TGGCTTCTGC GTCATCGTCA GTTTTCTTTT TCACTTGCAA AAGCTGAGCG GCCTGGATAA
            421 GAGGTTCCAG AGTCTCCTTG GCCCCACTGT TCATTAGATT CTTGTCACGT AGCCATTCTT
            481 CCAGTTGACT GACATTGTAC CTGATCTGCA TGCCCTTGCT CCAGGAGCAC ATGTCCTTGC
            541 GCAGGAGGAG GTTGTTTAGG GTGATGGCGC CCACAATGTA GAACATCTGT TTGACTACCT
            601 GCTTGATTAG CTCCGGGTCC ATGCCATGCT GACACATGAC TGAGTGGAAG GAGTTGAGCT
            661 GCCGAAGGAT GGAGTCCAGT GTGTAGGTGC CCTCATCTGC GATACTGGAG GTTCGTTTTC
            721 TGAGCCCTGT GGGCTTCACC CCAGATACGC CCTGAATTGT TTCATGCTCT AGCATACCTG
            781 AGACTATCAT TGGCTGAAGA ATGTTCTCTA ACACCCTCAC AAGCTGCTGA TAGATCTGAA
            841 TGGCCAAGTC ACTCAGTACT TGCCGATATT CAGCAAGGTC AAAATTGGTG AGGCAGTGCT
            901 CATTCTGGCG AGACGTGTTG TGCTTCATAA AGCCCTCCTC TCCACTATAT TGCTTCAAAC
            961 AGTGCAAAAA TCGACATGTG TTAGAGAGCC CAGATGAGAC AGTTTCAAAA TCGTCACCTC
           1021 TTTTTTCTTC AGACTTTTTT TGGAATGCTT GCTAATGATG ATGTCAGCAA TGGATCCTAC
          1081 CTTCCTGATT CAATCGATTC CAGAAAATAG TTCCAGGCCA T
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.

          2022.05.17

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