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pEF-FLAG-mouse myosin Va MC-tail (Melanocyte type)

Expression vector of mouse melanocyte-type myosin Va tail region (- exon B, + exon D and F).

Catalog number RDB15285
Resource name pEF-FLAG-mouse myosin Va MC-tail (Melanocyte type)
Clone info. Expression vector of mouse melanocyte (MC)-type myosin Va tail region (- exon B, + exon D and F), tagged with N-terminal FLAG, EF1 promoter. cDNA fragment 3161nt to 5989nt of NM_010864.2 is cloned.
Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : G to A substitution at 3481nt of NM_010864.2 (Gln, Synonym).
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo5a cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Myo5a (NCBI Gene 17918) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Fukuda, M. and Kuroda T. S., J. Biol. Chem., 277 (45): 43096-43103, 2002).
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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RDB15285 pEF-FLAG-mouse myosin Va MC-tail (Melanocyte type) DNA solution

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References and tips

Electronic file

Featured content

References

original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15285_A7Gop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15285_A7Goa.seq
>D04773A3_A7Go_1_EF1a-C_F_C07_07_ABI24.ab1
    1 CCAGTCATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCGCTCGCT CTGTGGAACG
  181 CTACAAGAAG CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA
  241 TGAGCAGAAT AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA
  301 CAACTCTGAG ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA
  361 AGCTAAGGTT GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA
  421 AGACCTGGAA CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA
  481 ACAGGAAACT GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA
  541 AAAGGAGACC CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA
  601 GAGGAAGTTA GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG
  661 GTATCAGAAC CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA
  721 AGAGATGACC CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA
  781 CAGCAGTAAT GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC
  841 ACCAAGAACA GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA
  901 GCTCCAGAAG CGTGTCACAG AGCTGGAAAC AGGAGAAGCA GCTGATGCAG GATGAGCTGG
  961 ACCGCAAGGG AGGGAGCAGG TGTTCCCGCA GCAAGGCAAA GGAAGAAGAA AGGGCCACAG
 1021 ATAGAGGAGC TGACTAGAGT ATGAGTCTTC TCAGCGTCAG GAACTGCAGT TCAGAAACCA
1081 AAAAACTGCA AAAAACGGAG CC
//
Primer: hCGSF_R RDB15285_A7Gob.seq
>D04773A3_A7Go_1_hGCSF_R_C08_08_ABI24.ab1
    1 CTCACTGATC TCTCAGGGTC CAGGTGGGGA CCCTCACTCT AGAGCGGCCG CTATGGTCGA
   61 CGGTATCGAT AAGCTTGATA TCGAATTCGA TTCAGACCCG TGCGATGAAG CCCAGGCCCA
  121 GGCTGGCTGG GATCTGGATG GTTTCTAGGG CTAGGGAGGA TGGGTTAAAG GGAAAAGTGA
  181 CAGGAAAGAT GTGTTTTGCA TCCATGAGCA GCTGTGGAGA GTCTTTCCTG TCTCGTAACC
  241 GCATCTGTAT AGTGCGGATA AATGAAACTG AGACCCTTTC TTCAAACTCA TTAACCGGTG
  301 TGTACAGATT CAACACTTTG ACAATCTGGG CCGTGGTCAG GGCGTTGCAC ATGGAACAGA
  361 TGGCTTCTGC GTCATCGTCA GTTTTCTTTT TCACTTGCAA AAGCTGAGCG GCCTGGATAA
  421 GAGGTTCCAG AGTCTCCTTG GCCCCACTGT TCATTAGATT CTTGTCACGT AGCCATTCTT
  481 CCAGTTGACT GACATTGTAC CTGATCTGCA TGCCCTTGCT CCAGGAGCAC ATGTCCTTGC
  541 GCAGGAGGAG GTTGTTTAGG GTGATGGCGC CCACAATGTA GAACATCTGT TTGACTACCT
  601 GCTTGATTAG CTCCGGGTCC ATGCCATGCT GACACATGAC TGAGTGGAAG GAGTTGAGCT
  661 GCCGAAGGAT GGAGTCCAGT GTGTAGGTGC CCTCATCTGC GATACTGGAG GTTCGTTTTC
  721 TGAGCCCTGT GGGCTTCACC CCAGATACGC CCTGAATTGT TTCATGCTCT AGCATACCTG
  781 AGACTATCAT TGGCTGAAGA ATGTTCTCTA ACACCCTCAC AAGCTGCTGA TAGATCTGAA
  841 TGGCCAAGTC ACTCAGTACT TGCCGATATT CAGCAAGGTC AAAATTGGTG AGGCAGTGCT
  901 CATTCTGGCG AGACGTGTTG TGCTTCATAA AGCCCTCCTC TCCACTATAT TGCTTCAAAC
  961 AGTGCAAAAA TCGACATGTG TTAGAGAGCC CAGATGAGAC AGTTTCAAAA TCGTCACCTC
 1021 TTTTTTCTTC AGACTTTTTT TGGAATGCTT GCTAATGATG ATGTCAGCAA TGGATCCTAC
1081 CTTCCTGATT CAATCGATTC CAGAAAATAG TTCCAGGCCA T
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fukuda, M., Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J. Biol. Chem. 277 (45): 43096-43103 (2002). PMID 12221080.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl