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pEGFP-C1-human TBC1D10B (#RDB14995)

Expression vector of human TBC1 domain family member 10B (TBC1D10B), fused with N-terminal EGFP, CMV promoter.

Alternative name FLJ13130
EPI64B
Clone info. Expression vector of human TBC1 domain family member 10B (TBC1D10B), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D10B cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14995 pEGFP-C1-human TBC1D10B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D10B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14995).

Reference section:

Itoh, T., Fukuda, M., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14995_A7Egp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Sequence file: RDB14995_A7Ega.seq check
>D04556C7_A7Eg_2_EGFP-C2_A07_01_ABI24.ab1
    1 TTCCCAACGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGTCTGGGA CCTTGGAGTC
  121 CTTGGCGGAT GATGTGAGCT CCATGGGCTC AGATTCAGAG ATAAACGGGC TGGCCCTGCG
  181 CAAGACGGAC AAGTATGGCT TCCTTGGGGG CAGCCAGTAC TCGGGCAGCC TAGAGAGCTC
  241 CATTCCCGTG GACGTGGCTC GGCAGCGGGA GCTCAGATGG CTGGACATGT TCAGTAACTG
  301 GGATAAGTGG CTGTCACGGC GATTCCAGAA GGTGAAGCTG CGCTGCCGGA AGGGGATCCC
  361 CTCCTCTCTC AGAGCCAAAG CCTGGCAGTA CCTGTCTAAT AGCAAGGAAC TTCTGGAGCA
  421 GAACCCAGGA AAGTTTGAGG AGCTGGAACG GGCTCCTGGG GACCCCAAGT GGCTGGATGT
  481 GATTGAGAAG GACCTGCACC GCCAGTTCCC TTTCCACGAG ATGTTTGCTG CTCGAGGGGG
  541 GCATGGGCAA CAGGACCTGT ACCGAATCCT GAAGGCCTAC ACCATCTACC GGCCTGACGA
  601 GGGTTACTGC CAGGCCCAGG CCCCCGTGGC TGCGGTCCTG CTCATGCACA TGCCTGCGGA
  661 GCAAGCCTTT TGGTGCCTGG TGCAGATCTG CGACAAGTAC CTCCCAGGTT ACTACAGTGC
  721 AGGGCTGGAG GCCATTCAGC TGGACGGGGA GATCTTTTTT GCACTCCTGC GCCGGGCCTC
  781 CCCGCTGGCG CATCGCCACC TGCGGCGGCA GCGCATTGAC CCTGTGCTCT ACATGACGGA
  841 GTGGTTCATG TGCATCTTCG CCCGCACCCT GCCCTGGGCG TCGGTGCTGC GTGTCTGGGA
  901 CATGTTTTTT CTGTGAAAGG CGTTAAGATC ATCTTCCGGG TGGCCCCTGG GTCCTGCTGC
  961 GCCACACGCT GGGCTCAGTG GAGAAGCTGC GCTCCTGCCC AAGGCATGTA TGAGACCATG
 1021 GAGCCAGCCT GCGTAACCTG CCCCAGCAGT GCCATGGCAA GAGGACTTCT GCTGCATGAG
1081 TGACCGATCT GGCGGATGAC CGAAGGCAAC TGAATTGGAA GCCGGA
//
Primer: SV40polyA-R
Sequence file: RDB14995_A7Egb.seq check
>D04556C7_A7Eg_2_SV40polyA-R_A08_02_ABI24.ab1
    1 GGGAATGCAG TTTTTAGCAG TAAACCTCTA CAATGTGGTA TGGCTGATTA TGATCAGTTA
   61 TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACC TGCAGGCGGC CGCGAATTCA
  121 CTAGTGATTA GGGCAGAGGT CAGAAGTAAG CGTCCTGCCG GGCCTCGGCT GAGGGCCTGT
  181 CCCCACCATC ATGGGGGCCT GGGGGCCCAT CTGCCTTTCG ACGCAGCGAA AGCTTCCGGC
  241 CTTGAGCCTT CTTCTCCTGC TTCTGCCGCT CCTTCTCCTG CTTCTCTCGC TCCTTTTCCT
  301 GCTTCTCTCG CTCTTTCTCC TGCTTCTGCC GCTCCTTCTC CCGCTCCTTC TCCTGTTTCT
  361 GCCGCTCCTT CTCCTGTTTC TGCCGCTCCT TCTCCTGCTT CCGGGTCTCC TTGCTGGAAC
  421 CCAAGGGGGT GCTATTGCCA GTGGGTGAGG GAAGGGATGG ATGCAGTCCC TCAGCAGTGA
  481 CCACAGGCCC TGGGGCAGAC CCAGCACTGG CTCTGCGGAC GGGGGGCGGC GGGGACGGGG
  541 CCCCTCCAGC TGCCCGGGAG TCTCGGCTCT TGAGGCCAGG GAGGCTGAGG AGGCTGGAGG
  601 AGGGGCCCAG GGGTGGCTGT TGCCGCCGGC GCTCCTCGTG GATGGCCCGG GACCCATGCA
  661 GTCGCCGTGA GGGCCGATAC TGCAGCTCCC CCCGCGTTTC CCGCCACTTC TTGAGCTGGG
  721 CTGCATTCTC CCGCTCAATC AGTGCTTCTG TCACCGGCAG ATTGGTCACC TCATGCACCA
  781 GGAAGTCTTC CTGCATGCAC TGCTGGGGCA GGTTACGCAG CTGCTCCATG GTCTCATACA
  841 TGCCTTGGCA GGAGCGCAGC TTCTCCACTG AGCCCAGCGT GTGGCGCAGC AGGACCAGGG
  901 CCACCCGGGA AGATGATCTT AACGCCTTCA CAGAAAAACA TGTCCCAGAC ACGCAGCACC
  961 GACGCCCAGG GCAGGGTGCG GGCGAAGATG CACATGAACC CACTCCCGTC ATGTAGAGCA
 1021 CAGGTCAATG CGGCTGCCGG CGCAGTGGCG ATGCGCAGCC GGGGAGCCCG CGCAGAGTGC
1081 AAAAGATCTC CCGTCAGCTG AATTGGCATC CAAGAGCCCT GGTA
//
Primer: SV40pro_F_V2
Sequence file: RDB14995_A7Egc.seq check
>D04556C7_A7Eg_2_SV40pro_F_V2_A09_03_ABI24.ab1
    1 GTACGACTTT TTTGGAGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAGGT TGGGCTTCGG
  961 AATCGTTTTC CCGGGACGCC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGGA
 1021 GGTCTTCGCC CCACCCTAGG GGGAGGGCTA CCTGAAACAC GGGAGAAGAA CATACAGAAG
1081 AACCGCGCTA TGAACGGCAT AAAAAGGAAC CG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]