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pEF-T7-Syt XI (Syt11) (#RDB14691)

Expression vector of mouse synaptotagmin XI (Syt XI) [Syt 11].

Clone info. Expression vector of mouse synaptotagmin XI (Syt XI) [Syt 11], T7-tag, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Syt11 cDNA
Reference of insert sequence AB026808 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. J. Biol. Chem. 274, 31421-31427, 1999)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. J. Biol. Chem. 274, 31421-31427, 1999)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14691 pEF-T7-Syt XI (Syt11) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-Syt XI (Syt11) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14691).

Reference section:

Fukuda, M., Kanno, E., Mikoshiba, K., Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X. J. Biol. Chem. 274 (44): 31421-31427 (1999). PMID 10531343. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14691_A7Ddp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Sequence file: RDB14691_A7Dda.seq check
>D04472B9_A7Dd_2_EF1a-C_F_A08_02_ABI24.ab1
    1 TTATGGCATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGCGG CCGCGGGAAT
  121 TCGATTCCAC CATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGCGGA TCTATGGCTG
  181 AGATCACAAA TATACGCCCC AGCTTTGATG TGTCACCGGT AGCGGCTGGC CTCATCGGGG
  241 CCTCCGTGCT GGTGGTGTGT GTTTCAGTGA CTGTCTTTGT GTGGACGTGC TGCCACCAGC
  301 AGGCAGAGAA GAAGCACAAG ACCCCGCCAT ACAAGTTCAT TCATATGCTG AAAGGCATTA
  361 GCATCTACCC AGAGACCCTC AGCAACAAGA AGAAAATCAT CAAAGTTCGG AGAGACAAAG
  421 ATGGCCCTCG GAGGGAAAGT GGACGAGGGA ACCTGTTAAT AAACGCGGAG TCTGGCCTGC
  481 TGAGCCACGA CAAGGATCCC AGAGGGCCTA GCCCTGCATC TTGTATGGAC CAGTTACCCA
  541 TCAAAAGAGA CTATGGGGAA GAACTGAGGA GCCCCATGAC AAGCCTGACC CCTGGTGAGA
  601 GCAAAGCCAC CTCCCCATCC TCGCCAGAGG AAGACGTGAT GTTAGGCTCT CTTACCTTCT
  661 CCGTGGACTA CAACTTTCCC AAGAAAGCTC TGGTGGTGAC AATCCAAGAG GCTCACGGGC
  721 TGCCAGTGAT GGATGACCAG ACCCAGGGCT CCGATCCCTA CATCAAAATG ACCATTCTCC
  781 CGGACAAGCG GCATCGAGTG AAGACCCGAG TGCTTCGCAA GACGCTGGAC CCGGTGTTCG
  841 ATGAGACCTT CACCTTCTAC GGCATCCCGT ACAGCCAGCT GCAGGACCTG GTGCTGCATT
  901 TCCTGGTGCT CAGCTTTGAT CGATTCTCTC GGGATGACGT CATCGGAGAG GTCATGGTGC
  961 CATTGGCTGG AGTGGACCCC AGCACAGGCA AGTTCAGCTG ACCAGGGACA TCATCCAGAG
 1021 ACATTCAGAA GTGCATTAGC CAGAGGTACT CAGGGTATCT CTGTCATACC AGGCTCGTTC
1081 GCACAGAAGA ATGAACCAGT GGCTTTGGGC
//
Primer: hGCSF_R ()
Sequence file: RDB14691_A7Ddb.seq check
>D04472B9_A7Dd_2_hGCSF_R_A09_03_ABI24.ab1
    1 CTGGACATGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCGAATTC
   61 ACTAGTTTAG TACTCGCTCA GACTGTGCCA CTTGGCTATG GGCTTGCGGG GACTCTCGCA
  121 GACCTCTCTC CAGTGTTCCG CACCACTGGT TGTGACACTG TGTGCCCCCA AGATCAGCCT
  181 CCCTACCACC TCATTCTTAG TAGTGCGATC GAAGTCAATG ACAAGGAACT CGATGCTGAT
  241 ATCAGGCAGA AGGTCGGTGG GGATGTCATA GATGAACGAC TCATTGAAGA CGGGGTTCAA
  301 AGTGCACTTC TTTACATGGG TTTTCTTCTT GGCAATGCGT TTTCTGCCGT AGTAGACGTT
  361 CACCTTGACA TAAGGATTAC CTGAGAGACC GGTGATATCC ATCTTCGGCA AGTGTCTGGC
  421 TTTGAGGACC ACCACTGTCA TTCTCTGTGC GACGGGCTGG TATGACAGAG ATACCTGGAG
  481 TTCCCCTCTG CTAATGCACT TCTGAATGTT CCTCTTGATG ATGTCCCTGG TCAGCTGAAC
  541 CTTGCCTGTG CTGGGGTCCA CTCCAGCCAA TGGCACCATG ACCTCTCCGA TGACGTCATC
  601 CCGAGAGAAT CGATCAAAGC TGAGCACCAG GAAATGCAGC ACCAGGTCCT GCAGCTGGCT
  661 GTACGGGATG CCGTAGAAGG TGAAGGTCTC ATCGAACACC GGGTCCAGCG TCTTGCGAAG
  721 CACTCGGGTC TTCACTCGAT GCCGCTTGTC CGGGAGAATG GTCATTTTGA TGTAGGGATC
  781 GGAGCCCTGG GTCTGGTCAT CCATCACTGG CAGCCCGTGA GCCTCTTGGA TTGTCACCAC
  841 CAGAGCTTTC TTGGGAAAGT TGTAGTCCAC GGAGAAGGTA AGAGAGCCTA ACATCACGTC
  901 TTCCTCTGGC GAGGATGGGG AGGTGGCTTT GCTCTCACCA GGGGTCAGCT TGTCATGGGG
  961 CTCCTCAGTT CTTTCCCCAT AGTCTCTTTT GATGGGTAAC TGGTCCATAC AAGATGCAGG
 1021 GCTAGGCCTC CTGGGATCTG TCGTTGCTCA GCAGGCAGAC TCCGCGTTTA TTAACAGTAC
1081 TCGTCACTTC CCTCGAGGGT CAATCTTGTC TCTCCCTCCG AAACCATG
//
Primer:
Sequence file: RDB14691_A7Ddc.seq check
1
Primer:
Sequence file: RDB14691_A7Ddd.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X. J. Biol. Chem. 274 (44): 31421-31427 (1999). PMID 10531343. [link to RRC of NBRP]