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pBabe-hSTING(V155M_C88S_C91S) (#RDB14358)

Retroviral expression vector of human STING, V155M_C88S_C91S mutant.

Clone info. Mammalian retroviral vector for expressing human STING (mutation, V155M. dual mutation, C88S/C91S). Suppression of STING palmitoylation inhibits the gain-of-function phenotype in the SAVI–STING.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14358 pBabe-hSTING(V155M_C88S_C91S) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBabe-hSTING(V155M_C88S_C91S) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14358).

Reference section:

Mukai, K., Konno, H., Akiba, T., Uemura, T., Waguri, S., Kobayashi, T., Barber, G.N., Arai, H., Taguchi, T., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14358_A6J7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' ()
Sequence file: RDB14358_A6J7a.seq check
>D03866A8_A6J7_1_pBABE5'_H01_22_ABI24.ab1
    1 AGGGGTGCGG GGATCAGGTG TGGTGGTACG TAGGAATTCG CCGCCACCAT GCCCCACTCC
   61 AGCCTGCATC CATCCATCCC GTGTCCCAGG GGTCACGGGG CCCAGAAGGC AGCCTTGGTT
  121 CTGCTGAGTG CCTGCCTGGT GACCCTTTGG GGGCTAGGAG AGCCACCAGA GCACACTCTC
  181 CGGTACCTGG TGCTCCACCT AGCCTCCCTG CAGCTGGGAC TGCTGTTAAA CGGGGTCTGC
  241 AGCCTGGCTG AGGAGCTGCG CCACATCCAC TCCAGGTACC GGGGCAGCTA CTGGAGGACT
  301 GTGCGGGCCA GCCTGGGCAG CCCCCTCCGC CGTGGGGCCC TGTTGCTGCT GTCCATCTAT
  361 TTCTACTACT CCCTCCCAAA TGCGGTCGGC CCGCCCTTCA CTTGGATGCT TGCCCTCCTG
  421 GGCCTCTCGC AGGCACTGAA CATCCTCCTG GGCCTCAAGG GCCTGGCCCC AGCTGAGATC
  481 TCTGCAGTGT GTGAAAAAGG GAATTTCAAC ATGGCCCATG GGCTGGCATG GTCATATTAC
  541 ATCGGATATC TGCGGCTGAT CCTGCCAGAG CTCCAGGCCC GGATTCGAAC TTACAATCAG
  601 CATTACAACA ACCTGCTACG GGGTGCAGTG AGCCAGCGGC TGTATATTCT CCTCCCATTG
  661 GACTGTGGGG TGCCTGATAA CCTGAGTATG GCTGACCCCA AACATTCGCT TCCTGGATAA
  721 AACTGCCCCA GCAGACCGGT GACCATGCTG GCATCAAAGG ATCGGGTTTA CAGCAACAGC
  781 ATCTATGAGC TTTCTGGGAG AAACGGGCAG CGGGCGGGGC ACCTGTGTCC TGGAGTACGC
  841 CACCCCCTTG CAGACTTTGT TTGCCATGTC ACAATACAGT CAAGCTTGGC TTTAGCCGGG
  901 AAGAATAGGC TTTGAGCAGG CCCAAACTCT TTCTGCCCGG ACACTTGAAG GAACATCCTG
  961 GCAATTGCCC CCTGAAGTCT CAGAACAAAC TGCCGCCCTC ATTGCCTAAC CAGGGAACCC
 1021 TGCAGATGAC AGCAGCTTTC CTCGCCTGAT CCCCAGAAGG TTCTCCGGCA CCTGGCGGCC
1081 ATGAAAGAAA AGGAAAGAAA GGTTAT
//
Primer: PuroR-rev ()
Sequence file: RDB14358_A6J7b.seq check
>D03866A8_A6J7_1_PuroR-rev_H06_08_ABI08.ab1
    1 GATAGGCTTT TTGCAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT
   61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG
  121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA
  181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG
  241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG
  301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG
  361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG
  421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC
  481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT
  541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG
  601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG
  661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG
  721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC
  781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG
  841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC
  901 AGCCGCTGGC TCACTGCACC CCGTAGCAGG TTGTTGTAAT GCTGATTGTA AGTTCGAATC
  961 CGGGCCTGGA GCTCTGGCAG GATCAGCCGC AGATATCCGA TGTAATATGA CCATGCCAGC
 1021 CCCATGGGCC ATGTTGAAAT TTCCCTTTTT CACACACTGC AGAGGATCTC AGCTGGGGGC
 1081 CAGCCATGAG TCCAGGAGAT GATCAGTGCC TGCAGAGCCA AGAGGCAGGC CATCCAAGTG
1141 AAGGGTCCGG C
//
Primer: Amp_R ()
Sequence file: RDB14358_A6J7c.seq check
>D03866A8_A6J7_1_Amp_R_H03_24_ABI24.ab1
    1 CTCTTTTCAA TATTATTGAA GGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGCAGC CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
  181 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
  241 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
  301 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
  361 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
  421 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGGGCG CCAGTCCTCC GATTGACTGA
  481 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
  541 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
  601 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
  661 AGGCAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
  721 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
  781 GAACTGACGA GTTCTGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCAGGG ACTTTGGGGG
  841 CCGTTTTTGT GGCCCGACCT GAGGAAAGGG AGTCGATGTG GAATCCGACC CCGTCAGATA
  901 TGTGGGTTCT GGTAGGAGAC GAGAACCTAA AACAGTTCCC GCCCTCCGTC TGAATTTTTG
  961 CTTTCGGTTT GGAAACCGAA GCCCGCGCGT CTTGTCTGCT GCAGCGCTGC AGCATCGTCT
 1021 GTGTGTCTCT GTCTGACTGT GTTCTGTATT GTCTGAAATA GGCCCAGACT GGTAACCACT
1081 CCCTAGTTGA ACCTTGAGTC CACTGGAAGG ATGTGTTCGA AGGCCGGG
//
Primer: SV40pro_F_V2 ()
Sequence file: RDB14358_A6J7d.seq check
>D03866A8_A6J7_1_SV40pro_F_V2_F07_06_ABI08.ab1
    1 GTGCACTTTT TTGGAGGCTA GGCTTTTGCA AAAGCTTACC ATGACCGAGT ACAAGCCCAC
   61 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT
  121 CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC
  181 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC
  241 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT
  301 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA
  361 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC
  421 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG
  481 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA
  541 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG
  601 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA
  661 GCGCCCGACC GAAAGGAGCG CACGACCCCA TGCATCGATA AAATAAAAGA TTTTATTTAG
  721 TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT
  781 AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG
  841 GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC
  901 CTGCCCCGGC TCAGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT
  961 GTGGTAAGCA GTTCCTGCCC CGGGCTCAGG CCAAGAACAG ATGGGTCCCC AGATGCGGTC
 1021 CAGCCTCAGC AGTTTCTAGG AGACATCAGA TGTTTCCAAG GGTGCCCCAG AACTGAAATG
1081 ACTGTGCTAA TTGAACTAAG CATCAGTTCG CATTCTTCCG CCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217. [link to RRC of NBRP]