Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBabe-hSTING(V155M_C88S_C91S)

Retroviral expression vector of human STING, V155M_C88S_C91S mutant.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14358_A6J7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14358_A6J7a.seq
>D03866A8_A6J7_1_pBABE5'_H01_22_ABI24.ab1     1 AGGGGTGCGG GGATCAGGTG TGGTGGTACG TAGGAATTCG CCGCCACCAT GCCCCACTCC    61 AGCCTGCATC CATCCATCCC GTGTCCCAGG GGTCACGGGG CCCAGAAGGC AGCCTTGGTT   121 CTGCTGAGTG CCTGCCTGGT GACCCTTTGG GGGCTAGGAG AGCCACCAGA GCACACTCTC   181 CGGTACCTGG TGCTCCACCT AGCCTCCCTG CAGCTGGGAC TGCTGTTAAA CGGGGTCTGC   241 AGCCTGGCTG AGGAGCTGCG CCACATCCAC TCCAGGTACC GGGGCAGCTA CTGGAGGACT   301 GTGCGGGCCA GCCTGGGCAG CCCCCTCCGC CGTGGGGCCC TGTTGCTGCT GTCCATCTAT   361 TTCTACTACT CCCTCCCAAA TGCGGTCGGC CCGCCCTTCA CTTGGATGCT TGCCCTCCTG   421 GGCCTCTCGC AGGCACTGAA CATCCTCCTG GGCCTCAAGG GCCTGGCCCC AGCTGAGATC   481 TCTGCAGTGT GTGAAAAAGG GAATTTCAAC ATGGCCCATG GGCTGGCATG GTCATATTAC   541 ATCGGATATC TGCGGCTGAT CCTGCCAGAG CTCCAGGCCC GGATTCGAAC TTACAATCAG   601 CATTACAACA ACCTGCTACG GGGTGCAGTG AGCCAGCGGC TGTATATTCT CCTCCCATTG   661 GACTGTGGGG TGCCTGATAA CCTGAGTATG GCTGACCCCA AACATTCGCT TCCTGGATAA   721 AACTGCCCCA GCAGACCGGT GACCATGCTG GCATCAAAGG ATCGGGTTTA CAGCAACAGC   781 ATCTATGAGC TTTCTGGGAG AAACGGGCAG CGGGCGGGGC ACCTGTGTCC TGGAGTACGC   841 CACCCCCTTG CAGACTTTGT TTGCCATGTC ACAATACAGT CAAGCTTGGC TTTAGCCGGG   901 AAGAATAGGC TTTGAGCAGG CCCAAACTCT TTCTGCCCGG ACACTTGAAG GAACATCCTG   961 GCAATTGCCC CCTGAAGTCT CAGAACAAAC TGCCGCCCTC ATTGCCTAAC CAGGGAACCC  1021 TGCAGATGAC AGCAGCTTTC CTCGCCTGAT CCCCAGAAGG TTCTCCGGCA CCTGGCGGCC 1081 ATGAAAGAAA AGGAAAGAAA GGTTAT //
Primer: PuroR-rev Sequence file: RDB14358_A6J7b.seq
>D03866A8_A6J7_1_PuroR-rev_H06_08_ABI08.ab1     1 GATAGGCTTT TTGCAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT    61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG   121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA   181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG   241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG   301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG   361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG   421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC   481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT   541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG   601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG   661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG   721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC   781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG   841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC   901 AGCCGCTGGC TCACTGCACC CCGTAGCAGG TTGTTGTAAT GCTGATTGTA AGTTCGAATC   961 CGGGCCTGGA GCTCTGGCAG GATCAGCCGC AGATATCCGA TGTAATATGA CCATGCCAGC  1021 CCCATGGGCC ATGTTGAAAT TTCCCTTTTT CACACACTGC AGAGGATCTC AGCTGGGGGC  1081 CAGCCATGAG TCCAGGAGAT GATCAGTGCC TGCAGAGCCA AGAGGCAGGC CATCCAAGTG 1141 AAGGGTCCGG C //
Primer: Amp_R Sequence file: RDB14358_A6J7c.seq
>D03866A8_A6J7_1_Amp_R_H03_24_ABI24.ab1     1 CTCTTTTCAA TATTATTGAA GGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT    61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC   121 CACCTGCAGC CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC   181 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC   241 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG   301 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT   361 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC   421 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGGGCG CCAGTCCTCC GATTGACTGA   481 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT   541 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA   601 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG   661 AGGCAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT   721 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG   781 GAACTGACGA GTTCTGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCAGGG ACTTTGGGGG   841 CCGTTTTTGT GGCCCGACCT GAGGAAAGGG AGTCGATGTG GAATCCGACC CCGTCAGATA   901 TGTGGGTTCT GGTAGGAGAC GAGAACCTAA AACAGTTCCC GCCCTCCGTC TGAATTTTTG   961 CTTTCGGTTT GGAAACCGAA GCCCGCGCGT CTTGTCTGCT GCAGCGCTGC AGCATCGTCT  1021 GTGTGTCTCT GTCTGACTGT GTTCTGTATT GTCTGAAATA GGCCCAGACT GGTAACCACT 1081 CCCTAGTTGA ACCTTGAGTC CACTGGAAGG ATGTGTTCGA AGGCCGGG //
Primer: SV40pro_F_V2 Sequence file: RDB14358_A6J7d.seq
>D03866A8_A6J7_1_SV40pro_F_V2_F07_06_ABI08.ab1     1 GTGCACTTTT TTGGAGGCTA GGCTTTTGCA AAAGCTTACC ATGACCGAGT ACAAGCCCAC    61 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT   121 CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC   181 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC   241 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT   301 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA   361 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC   421 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG   481 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA   541 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG   601 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA   661 GCGCCCGACC GAAAGGAGCG CACGACCCCA TGCATCGATA AAATAAAAGA TTTTATTTAG   721 TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT   781 AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG   841 GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC   901 CTGCCCCGGC TCAGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT   961 GTGGTAAGCA GTTCCTGCCC CGGGCTCAGG CCAAGAACAG ATGGGTCCCC AGATGCGGTC  1021 CAGCCTCAGC AGTTTCTAGG AGACATCAGA TGTTTCCAAG GGTGCCCCAG AACTGAAATG 1081 ACTGTGCTAA TTGAACTAAG CATCAGTTCG CATTCTTCCG CCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl