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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsHIF1A

Expression vector of human HIF1A.

Catalog number RDB06378
Resource name pCMFlag_hsHIF1A
Alternative name SET-0173_7
Clone info. Expression vector of human HIF1A. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano BC012527. PCR cloning, forward primer: gagggcgccggcggcgcgaa; reverse primer: gctcagttaacttgatccaaagctctg.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human hypoxia-inducible factor 1 alpha subunit (HIF1A) cDNA
Depositor|Developer DNA Bank, |
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External Database
Human hypoxia-inducible factor 1 alpha subunit (HIF1A)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB06378 pCMFlag_hsHIF1A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06378.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16934_A9Bfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB16934_A9Bfa.seq check
          >06378_16934_A9Bf_1_CMV-Forward_B09_02_ABI08.ab1
              1 TGTTTCCAAC CTCCCGGCCC CCTTTTGGAC GCAAATGGGC CGGGTAGGCG TGTACGGTGG
             61 GAGGTCTATA TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC
            121 GAAATTAATA CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC
            181 GATGACGATA AGGCGGCCGA GGGCGCCGGC GGCGCGAACG ACAAGAAAAA GATAAGTTCT
            241 GAACGTCGAA AAGAAAAGTC TCGAGATGCA GCCAGATCTC GGCGAAGTAA AGAATCTGAA
            301 GTTTTTTATG AGCTTGCTCA TCAGTTGCCA CTTCCACATA ATGTGAGTTC GCATCTTGAT
            361 AAGGCCTCTG TGATGAGGCT TACCATCAGC TATTTGCGTG TGAGGAAACT TCTGGATGCT
            421 GGTGATTTGG ATATTGAAGA TGACATGAAA GCACAGATGA ATTGCTTTTA TTTGAAAGCC
            481 TTGGATGGTT TTGTTATGGT TCTCACAGAT GATGGTGACA TGATTTACAT TTCTGATAAT
            541 GTGAACAAAT ACATGGGATT AACTCAGTTT GAACTAACTG GACACAGTGT GTTTGATTTT
            601 ACTCATCCAT GTGACCATGA GGAAATGAGA GAAATGCTTA CACACAGAAA TGGCCTTGTG
            661 AAAAAGGGTA AAGAACAAAA ACACACAGCG AAGCTTTTTT CTCAGAATGA AGTGTACCCT
            721 AACTAGCCGA GGAAGAACTA TGAACATAAA GTCTGCAACA TGGGAAGGTA TTGCACTGCA
            781 CAGGCCACAT TTCACGTATA TGATACCAAC AGTAACCAAC CTCAGTGTGG GGTATAAGAA
            841 AACCACCTAT GACCCTGCTT TGGTGCTGAT TTGTGGAACC CATTCCTCAC CCATCAAATA
            901 TTGAAAATTC CTTTTAGATA AGCAAGACGT TTCCTCAGTC GAACACAGCC TGGATATGAA
            961 AATTTTTCTT ATTTGTGATG AAAGAATTAC CGAATTGATG GGAATAATGA ACCCAGAAGA
           1021 CCTTTTAGGC CGGCTCAAAT TTTAGTGAAT ATTATCCATG CTTTTTGGAC TTCGTGAATT
           1081 CATTCTGACC GAGACCTTCA TTCAGTGATT ATGTTTACCA AGAGAACAAA GTCTCATCCA
           1141 CCAGGGAACA GTTACCAAGG AATGGCCTGT GCCAAAAGGA GGTGGGATTA TTGTTTCTCT
          1201 GTGGCGGGTG TTGAGAAAAA CACTCTATCA GAGGCACAGA CG
          //
          Primer: BGH_rev2
          Sequence file: RDB16934_A9Bfb.seq check
          >06378_16934_A9Bf_1_BGH_rev2_C09_03_ABI08.ab1
              1 CATAAAGGAA AAGGCCACGG GGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG
             61 CACAGTCGAG GCTGATCAGC GAGCTCTAGC ATTTAGGTGA CACTATAGAA TAGGGCCCTC
            121 TAGATGCATG CTCGAGCGGC CGCTCAGTTA ACTTGATCCA AAGCTCTGAG TAATTCTTCA
            181 CCCTGCAGTA GGTTTCTGCT GCCTTGTATA GGAGCATTAA CTTCACAATC ATAACTGGTC
            241 AGCTGTGGTA ATCCACTTTC ATCCATTGAT TGCCCCAGCA GTCTACATGC TAAATCAGAG
            301 GGTATTAAAA TAATTGTCTT TTGCTCCATT CCATTCTGTT CACTAGATTT GCATCCTTTT
            361 ACACGTTTCC AAGAAAGTGA TGTAGTAGCT GCATGATCGT CTGGCTGCTG TAATAATGTT
            421 CCAATTCCTA CTGCTTGAAA AAGTGAACCA TCATGTTCCA TTTTTCGCTT TCTCTGAGCA
            481 TTCTGCAAAG CTAGTATCTT TGGATTTAGT TCTTCCTCAG GAACTGTAGT TCTTTGACTC
            541 AAAGCGACAG ATAACACGTT AGGGCTTCTT GGATGAGATT TTTCTGTCTG TTCTATGACT
            601 CCTTTTCCTG CTCTGTTTGG TGAGGCTGTC CGACTTTGAG TATCTCTATA TGGTGATGAT
            661 GTGGCACTAG TAGTTTCTTT ATGTATGTGG GTAGGAGATG GAGATGCAAT CAATATTTTA
            721 ATGTCTTCCA TACGGTCTTT TGTCACTGTT TTTAATTCAT CAGTGGTGGC AGTGGTAGTG
            781 GTGGCATTAG CAGTAGGTTC TTGTATTTGA GTCTGCTGGA ATACTGGTAA CTGTGCTTTT
            841 GAGGACTTTG CGCTTTCAGG GGCTTGCGGA ACTGCTTTCT AATGGGTGAC ACTTGATCGA
            901 AAGGAACGTA ACTGGAAGTC ATTCATCCAA TTTGGGGATA TAGGGAGCTA AACATCCTCC
            961 CAATTCTAAA TCCTGGTGGT CCTGAAATTA GAAAAATGGG TTTCTTTGCC TTCCTGTGTC
           1021 CTTCCACCAA AAAGTTTTTT TCTCCCAATT TCCAAACTTT GGATTTCGTG TGACCATTAT
           1081 CACTATGTCG CAATAGAAAG CAATAATTTC ACCTGGGACT TATTAAGGGT CTTCAGGGTG
          1141 G
          //
          Primer: SV40pro_F_
          Sequence file: RDB16934_A9Bfc.seq check
          >06378_16934_A9Bf_1_SV40pro_F_D09_04_ABI08.ab1
              1 ACCTCGGGGC TGAGCTTTTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
             61 CAAAAAGCTC CCGGGAGCTT GGATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
            121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
            181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
            241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
            301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
            361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
            421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
            481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
            541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
            601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
            661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
            721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGGCGGACC
            781 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAAATGG
            841 GCTGAACCGC TTCCTCGTGG CTTTACGGTA TCGCCGCTCC CGATTCGCAA GCGCATCGCC
            901 CTTCTATCGC CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG
            961 ACCAAGCGAA CGCCCCAACC TGGCCATCAC GAGATTTTCG ATTTCCACCG GCCGGCCTTC
           1021 TAATGGAAAG GGTTTGGGGC TTTCGGGATT TCGTTTTTCC GGGGACGGCC GGCTGGGAAT
           1081 GAATTCCCTC CCGAGCCGCG GGGGGATCTC AATGCTGGGA AGTTCCCTTT CGCCCACCCC
          1141 TAACCTTGGT TTAATGGCAA GCCTTTAATA ATGGCTTACC AAGATGTAAA GACGAAGTA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Yoshida, T., Transcriptional upregulation of HIF-1 alpha by NF-kappaB/p65 and its associations with beta-catenin/p300 complexes in endometrial carcinoma cells. Lab. Invest., 93 (11): 1184-1193 (2013). PMID 24042437.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl