Expression vector of human HIF1A.
Alternative name | SET-0173_7 |
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Clone info. | Expression vector of human HIF1A. Expression was confirmed by Western blotting with anti-FLAG antibody. |
Comment | Derived from Sugano BC012527. PCR cloning, forward primer: gagggcgccggcggcgcgaa; reverse primer: gctcagttaacttgatccaaagctctg. |
Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
Size of vector backbone | 5.5 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
Gene/insert name | Human hypoxia-inducible factor 1 alpha subunit (HIF1A) cDNA |
Depositor|Developer | DNA Bank, | |
Remarks, protocol and/or map (pdf) | RDB06378.pdf |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB06378 | pCMFlag_hsHIF1A | DNA solution |
Materials & Methods section:
The pCMFlag_hsHIF1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06378). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16934_A9Bfp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV-Forward () Sequence file: RDB16934_A9Bfa.seq |
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>06378_16934_A9Bf_1_CMV-Forward_B09_02_ABI08.ab1 1 TGTTTCCAAC CTCCCGGCCC CCTTTTGGAC GCAAATGGGC CGGGTAGGCG TGTACGGTGG 61 GAGGTCTATA TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC 121 GAAATTAATA CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC 181 GATGACGATA AGGCGGCCGA GGGCGCCGGC GGCGCGAACG ACAAGAAAAA GATAAGTTCT 241 GAACGTCGAA AAGAAAAGTC TCGAGATGCA GCCAGATCTC GGCGAAGTAA AGAATCTGAA 301 GTTTTTTATG AGCTTGCTCA TCAGTTGCCA CTTCCACATA ATGTGAGTTC GCATCTTGAT 361 AAGGCCTCTG TGATGAGGCT TACCATCAGC TATTTGCGTG TGAGGAAACT TCTGGATGCT 421 GGTGATTTGG ATATTGAAGA TGACATGAAA GCACAGATGA ATTGCTTTTA TTTGAAAGCC 481 TTGGATGGTT TTGTTATGGT TCTCACAGAT GATGGTGACA TGATTTACAT TTCTGATAAT 541 GTGAACAAAT ACATGGGATT AACTCAGTTT GAACTAACTG GACACAGTGT GTTTGATTTT 601 ACTCATCCAT GTGACCATGA GGAAATGAGA GAAATGCTTA CACACAGAAA TGGCCTTGTG 661 AAAAAGGGTA AAGAACAAAA ACACACAGCG AAGCTTTTTT CTCAGAATGA AGTGTACCCT 721 AACTAGCCGA GGAAGAACTA TGAACATAAA GTCTGCAACA TGGGAAGGTA TTGCACTGCA 781 CAGGCCACAT TTCACGTATA TGATACCAAC AGTAACCAAC CTCAGTGTGG GGTATAAGAA 841 AACCACCTAT GACCCTGCTT TGGTGCTGAT TTGTGGAACC CATTCCTCAC CCATCAAATA 901 TTGAAAATTC CTTTTAGATA AGCAAGACGT TTCCTCAGTC GAACACAGCC TGGATATGAA 961 AATTTTTCTT ATTTGTGATG AAAGAATTAC CGAATTGATG GGAATAATGA ACCCAGAAGA 1021 CCTTTTAGGC CGGCTCAAAT TTTAGTGAAT ATTATCCATG CTTTTTGGAC TTCGTGAATT 1081 CATTCTGACC GAGACCTTCA TTCAGTGATT ATGTTTACCA AGAGAACAAA GTCTCATCCA 1141 CCAGGGAACA GTTACCAAGG AATGGCCTGT GCCAAAAGGA GGTGGGATTA TTGTTTCTCT 1201 GTGGCGGGTG TTGAGAAAAA CACTCTATCA GAGGCACAGA CG // |
Primer: BGH_rev2 () Sequence file: RDB16934_A9Bfb.seq |
>06378_16934_A9Bf_1_BGH_rev2_C09_03_ABI08.ab1 1 CATAAAGGAA AAGGCCACGG GGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG 61 CACAGTCGAG GCTGATCAGC GAGCTCTAGC ATTTAGGTGA CACTATAGAA TAGGGCCCTC 121 TAGATGCATG CTCGAGCGGC CGCTCAGTTA ACTTGATCCA AAGCTCTGAG TAATTCTTCA 181 CCCTGCAGTA GGTTTCTGCT GCCTTGTATA GGAGCATTAA CTTCACAATC ATAACTGGTC 241 AGCTGTGGTA ATCCACTTTC ATCCATTGAT TGCCCCAGCA GTCTACATGC TAAATCAGAG 301 GGTATTAAAA TAATTGTCTT TTGCTCCATT CCATTCTGTT CACTAGATTT GCATCCTTTT 361 ACACGTTTCC AAGAAAGTGA TGTAGTAGCT GCATGATCGT CTGGCTGCTG TAATAATGTT 421 CCAATTCCTA CTGCTTGAAA AAGTGAACCA TCATGTTCCA TTTTTCGCTT TCTCTGAGCA 481 TTCTGCAAAG CTAGTATCTT TGGATTTAGT TCTTCCTCAG GAACTGTAGT TCTTTGACTC 541 AAAGCGACAG ATAACACGTT AGGGCTTCTT GGATGAGATT TTTCTGTCTG TTCTATGACT 601 CCTTTTCCTG CTCTGTTTGG TGAGGCTGTC CGACTTTGAG TATCTCTATA TGGTGATGAT 661 GTGGCACTAG TAGTTTCTTT ATGTATGTGG GTAGGAGATG GAGATGCAAT CAATATTTTA 721 ATGTCTTCCA TACGGTCTTT TGTCACTGTT TTTAATTCAT CAGTGGTGGC AGTGGTAGTG 781 GTGGCATTAG CAGTAGGTTC TTGTATTTGA GTCTGCTGGA ATACTGGTAA CTGTGCTTTT 841 GAGGACTTTG CGCTTTCAGG GGCTTGCGGA ACTGCTTTCT AATGGGTGAC ACTTGATCGA 901 AAGGAACGTA ACTGGAAGTC ATTCATCCAA TTTGGGGATA TAGGGAGCTA AACATCCTCC 961 CAATTCTAAA TCCTGGTGGT CCTGAAATTA GAAAAATGGG TTTCTTTGCC TTCCTGTGTC 1021 CTTCCACCAA AAAGTTTTTT TCTCCCAATT TCCAAACTTT GGATTTCGTG TGACCATTAT 1081 CACTATGTCG CAATAGAAAG CAATAATTTC ACCTGGGACT TATTAAGGGT CTTCAGGGTG 1141 G // |
Primer: SV40pro_F_ () Sequence file: RDB16934_A9Bfc.seq |
>06378_16934_A9Bf_1_SV40pro_F_D09_04_ABI08.ab1 1 ACCTCGGGGC TGAGCTTTTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 61 CAAAAAGCTC CCGGGAGCTT GGATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGGCGGACC 781 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAAATGG 841 GCTGAACCGC TTCCTCGTGG CTTTACGGTA TCGCCGCTCC CGATTCGCAA GCGCATCGCC 901 CTTCTATCGC CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG 961 ACCAAGCGAA CGCCCCAACC TGGCCATCAC GAGATTTTCG ATTTCCACCG GCCGGCCTTC 1021 TAATGGAAAG GGTTTGGGGC TTTCGGGATT TCGTTTTTCC GGGGACGGCC GGCTGGGAAT 1081 GAATTCCCTC CCGAGCCGCG GGGGGATCTC AATGCTGGGA AGTTCCCTTT CGCCCACCCC 1141 TAACCTTGGT TTAATGGCAA GCCTTTAATA ATGGCTTACC AAGATGTAAA GACGAAGTA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
user_report | Yoshida, T., Transcriptional upregulation of HIF-1 alpha by NF-kappaB/p65 and its associations with beta-catenin/p300 complexes in endometrial carcinoma cells. Lab. Invest., 93 (11): 1184-1193 (2013). PMID 24042437. [link to RRC of NBRP] |
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