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Smad4-pcDNA1.1Amp (#RDB02854)

Expression vector of human SMAD4

Alternative name Smad4 cDNA
Clone info. Expression vector of human SMAD4 regulated by CMV promoter. PCR product. Expression was confirmed by the depositor in mammalian cells.
Vector backbone pcDNA1.1Amp (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name Human DPC4/Smad4 cDNA
Depositor|Developer Hagiwara, Koichi |
 
Sequence RDB02854z.seq
Additional map
Remarks, protocol and/or map (pdf) RDB02854.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Biotechniques, 25, 554-555 (1998)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biotechniques, 25, 554-555 (1998))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02854 Smad4-pcDNA1.1Amp DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Smad4-pcDNA1.1Amp was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02854).

Reference section:

Hagiwara, K., Freeman, A.A., McMenamin, M.G., Harris, C.C., Screening cloned PCR fragments by restriction endonuclease finger-printing to obtain wild-type sequences. Biotechniques, 25, 554-555 (1998). PMID 9793631. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13955_A6Bgp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13955_A6Bga.seq check
>02854_13955_A6Bg_CMV-Forward_C12_09.ab1
    1 AGTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TTGGTACCGA GCTCGGATCC ACTAGTAACG GCCGCCAGTG
  181 TGCTGGAATT CTTTTCCTTG CAACGTTAGC TGTTGTTTTT CACTGTTTCC AAAGGATCAA
  241 AATTGCTTCA GAAATTGGAG ACATATTTGA TTTAAAAGGA AAAACTTGAA CAAATGGACA
  301 ATATGTCTAT TACGAATACA CCAACAAGTA ATGATGCCTG TCTGAGCATT GTGCATAGTT
  361 TGATGTGCCA TAGACAAGGT GGAGAGAGTG AAACATTTGC AAAAAGAGCA ATTGAAAGTT
  421 TGGTAAAGAA GCTGAAGGAG AAAAAAGATG AATTGGATTC TTTAATAACA GCTATAACTA
  481 CAAATGGAGC TCATCCTAGT AAATGTGTTA CCATACAGAG AACATTGGAT GGGAGGCTTC
  541 AGGTGGCTGG TCGGAAAGGA TTTCCTCATG TGATCTATGC CCGTCTCTGG AGGTGGCCTG
  601 ATCTTCACAA AAATGAACTA AAACATGTTA AATATTGTCA GTATGCGTTT GACTTAAAAT
  661 GTGATAGTGT CTGTGTGAAT CCATATCACT ACGAACGAGT TGTATCACCT GGAATTGATC
  721 TCTCAGGATT AACACTGCAG AGTAATGCTC CATCAAGTAT GATGGTGAAG GATGAATATG
  781 TGCATGACTT TGAGGGACAG CCATCGTTGT CCACTGAAGG ACATTCAATT CAAACCATCC
  841 AGCATCCACC AAGTAATCGT GCATCGACAG AGACATACAG CACCCCAGCT CTGTTAGCCC
  901 CATCTGAGTC TAATGCTACC AGCACTGCCC AACTTTCCCA AACATTCCCT GTGGGCTTTC
  961 CACAAAGTCA GCCCTGCCCA GTATACTTGG GGGGGGGCAG CCCATAGTTG GAAGGAACTG
 1021 ATGCAAGATA GCAATCAACG TCTTCAGTCA AGGACAGCAG CAGATCATTA CTGATCAGCC
1081 AGCTACGTTA
//
Primer: pMAMneo-Reverse
Sequence file: RDB13955_A6Bgb.seq check
>02854_13955_A6Bg_pMAMneo-Reverse_D12_12.ab1
    1 TGTCATATGT CCACCACAGA AGTAAGGTTC CTTCACAAAG ATCCTCTAGA TGCATGCTCG
   61 AGTCTCCAAC GGTAAAAGAC CTCAGTCTAA AGGTTGTGGG TCTGCAATCG GCATGGTATG
  121 AAGTACTTCG TCTAGGAGCT GGAGGGCCCG GTGTAAGTGA ATTTCAATCC AGCAAGGTGT
  181 TTCTTTGATG CTCTGTCTTG GGTAATCCGG TCCCCAGCCT TTCACAAAAC TCATCCTGAG
  241 TATGCATAAG CGACGAAGGT CATCAACACC AATTCCAGCA GCAGCTGACA GACTGATAGC
  301 TGGAGCTATT CCACCTACTG ATCCTGGGCC AGGGATGTTT CCTGCCACGG CTGCTGCCTG
  361 GGCAGCTGCT GCAGCTTGTG CAGTAGCCGC CTGCTGCTGC ATCTGTCGAT GACACTGACG
  421 CAAATCAAAG ACCTTTATAT ATGCACTTGG GTAGATCTTA TGAACAGCAT CTCCAGGTGC
  481 ACGCCCAGCT TCTCTGTCTA AGTAGTAACT CTGTACAAAG ACCGCGTGGT CACTAAGGCA
  541 CCTGACCCAA ACATCACCTT CACCTTTACA TTCCAACTGC ACACCTTTGC CTATGTGCAA
  601 CCTTGCTCTC TCAATGGCTT CTGTCCTGTG GACATTGGAG AGTTGACCCA AACAAAAGCG
  661 ATCTCCTCCA GAAGGGTCCA CGTATCCATC AACAGTAACA ATAGGGCAGC TTGAAGGAAC
  721 CTTAAATGTC TCTCCTACCT GAACATCCAT TTCAAAGTAA GCAATGGAAC ACCAATACTC
  781 AGGAGCAGGA TGATTGGAAA TGGGAGGCTG GAATGCAAGC TCATTGTGAA CAGGCCAGTA
  841 ATGTCCGGGA TGGGGCGGCA TAGGCGGGTG GTGCTGAAGA TGGCCGTTTT GGTGGTGAGG
  901 CAAATTATGT GTGTATGGTG CAGTCCTACT TCCAGTCCAG GTGGTAGTGC TGTTTATGAT
  961 GGTAAAGTAG CTGGCTGACC CAGTAAATCC CATTTCTGCT GCTGGTCCTT GGCTGAGCCC
 1021 TGATGCTAAT CCTGCCACCA GTCCCTTCAC TAATGTCTGC CCCCCAAGTA TACTGGCAGG
1081 CTTGACCATG G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hagiwara, K., Screening cloned PCR fragments by restriction endonuclease finger-printing to obtain wild-type sequences. Biotechniques, 25, 554-555 (1998). PMID 9793631. [link to RRC of NBRP]