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Smad4-pcDNA1.1Amp

Expression vector of human SMAD4

Catalog number RDB02854
Resource name Smad4-pcDNA1.1Amp
Alternative name Smad4 cDNA
Clone info. Expression vector of human SMAD4 regulated by CMV promoter. PCR product. Expression was confirmed by the depositor in mammalian cells.
Vector backbone pcDNA1.1Amp (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name Human DPC4/Smad4 cDNA
Depositor|Developer Hagiwara, Koichi |
Other clones in our bank
External Database
Human DPC4/Smad4
          Reference sequence
            
           
          Sequence RDB02854z.seq
          Remarks, protocol and/or map (gif) RDB02854.gif
          Remarks, protocol and/or map (pdf) RDB02854.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Biotechniques, 25, 554-555 (1998)).
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          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biotechniques, 25, 554-555 (1998))。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB02854 Smad4-pcDNA1.1Amp DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Smad4-pcDNA1.1Amp was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02854).

          Reference section:

          Hagiwara, K., Freeman, A.A., McMenamin, M.G., Harris, C.C., Screening cloned PCR fragments by restriction endonuclease finger-printing to obtain wild-type sequences. Biotechniques, 25, 554-555 (1998). PMID 9793631. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13955_A6Bgp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13955_A6Bga.seq check
          >02854_13955_A6Bg_CMV-Forward_C12_09.ab1
              1 AGTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TTGGTACCGA GCTCGGATCC ACTAGTAACG GCCGCCAGTG
            181 TGCTGGAATT CTTTTCCTTG CAACGTTAGC TGTTGTTTTT CACTGTTTCC AAAGGATCAA
            241 AATTGCTTCA GAAATTGGAG ACATATTTGA TTTAAAAGGA AAAACTTGAA CAAATGGACA
            301 ATATGTCTAT TACGAATACA CCAACAAGTA ATGATGCCTG TCTGAGCATT GTGCATAGTT
            361 TGATGTGCCA TAGACAAGGT GGAGAGAGTG AAACATTTGC AAAAAGAGCA ATTGAAAGTT
            421 TGGTAAAGAA GCTGAAGGAG AAAAAAGATG AATTGGATTC TTTAATAACA GCTATAACTA
            481 CAAATGGAGC TCATCCTAGT AAATGTGTTA CCATACAGAG AACATTGGAT GGGAGGCTTC
            541 AGGTGGCTGG TCGGAAAGGA TTTCCTCATG TGATCTATGC CCGTCTCTGG AGGTGGCCTG
            601 ATCTTCACAA AAATGAACTA AAACATGTTA AATATTGTCA GTATGCGTTT GACTTAAAAT
            661 GTGATAGTGT CTGTGTGAAT CCATATCACT ACGAACGAGT TGTATCACCT GGAATTGATC
            721 TCTCAGGATT AACACTGCAG AGTAATGCTC CATCAAGTAT GATGGTGAAG GATGAATATG
            781 TGCATGACTT TGAGGGACAG CCATCGTTGT CCACTGAAGG ACATTCAATT CAAACCATCC
            841 AGCATCCACC AAGTAATCGT GCATCGACAG AGACATACAG CACCCCAGCT CTGTTAGCCC
            901 CATCTGAGTC TAATGCTACC AGCACTGCCC AACTTTCCCA AACATTCCCT GTGGGCTTTC
            961 CACAAAGTCA GCCCTGCCCA GTATACTTGG GGGGGGGCAG CCCATAGTTG GAAGGAACTG
           1021 ATGCAAGATA GCAATCAACG TCTTCAGTCA AGGACAGCAG CAGATCATTA CTGATCAGCC
          1081 AGCTACGTTA
          //
          Primer: pMAMneo-Reverse
          Sequence file: RDB13955_A6Bgb.seq check
          >02854_13955_A6Bg_pMAMneo-Reverse_D12_12.ab1
              1 TGTCATATGT CCACCACAGA AGTAAGGTTC CTTCACAAAG ATCCTCTAGA TGCATGCTCG
             61 AGTCTCCAAC GGTAAAAGAC CTCAGTCTAA AGGTTGTGGG TCTGCAATCG GCATGGTATG
            121 AAGTACTTCG TCTAGGAGCT GGAGGGCCCG GTGTAAGTGA ATTTCAATCC AGCAAGGTGT
            181 TTCTTTGATG CTCTGTCTTG GGTAATCCGG TCCCCAGCCT TTCACAAAAC TCATCCTGAG
            241 TATGCATAAG CGACGAAGGT CATCAACACC AATTCCAGCA GCAGCTGACA GACTGATAGC
            301 TGGAGCTATT CCACCTACTG ATCCTGGGCC AGGGATGTTT CCTGCCACGG CTGCTGCCTG
            361 GGCAGCTGCT GCAGCTTGTG CAGTAGCCGC CTGCTGCTGC ATCTGTCGAT GACACTGACG
            421 CAAATCAAAG ACCTTTATAT ATGCACTTGG GTAGATCTTA TGAACAGCAT CTCCAGGTGC
            481 ACGCCCAGCT TCTCTGTCTA AGTAGTAACT CTGTACAAAG ACCGCGTGGT CACTAAGGCA
            541 CCTGACCCAA ACATCACCTT CACCTTTACA TTCCAACTGC ACACCTTTGC CTATGTGCAA
            601 CCTTGCTCTC TCAATGGCTT CTGTCCTGTG GACATTGGAG AGTTGACCCA AACAAAAGCG
            661 ATCTCCTCCA GAAGGGTCCA CGTATCCATC AACAGTAACA ATAGGGCAGC TTGAAGGAAC
            721 CTTAAATGTC TCTCCTACCT GAACATCCAT TTCAAAGTAA GCAATGGAAC ACCAATACTC
            781 AGGAGCAGGA TGATTGGAAA TGGGAGGCTG GAATGCAAGC TCATTGTGAA CAGGCCAGTA
            841 ATGTCCGGGA TGGGGCGGCA TAGGCGGGTG GTGCTGAAGA TGGCCGTTTT GGTGGTGAGG
            901 CAAATTATGT GTGTATGGTG CAGTCCTACT TCCAGTCCAG GTGGTAGTGC TGTTTATGAT
            961 GGTAAAGTAG CTGGCTGACC CAGTAAATCC CATTTCTGCT GCTGGTCCTT GGCTGAGCCC
           1021 TGATGCTAAT CCTGCCACCA GTCCCTTCAC TAATGTCTGC CCCCCAAGTA TACTGGCAGG
          1081 CTTGACCATG G
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Hagiwara, K., Screening cloned PCR fragments by restriction endonuclease finger-printing to obtain wild-type sequences. Biotechniques, 25, 554-555 (1998). PMID 9793631. [link to RRC of NBRP]

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl