Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAMC3.0 (#RDB01067)

Human amyloid precursor 3 cDNA

Alternative name Human amyloid precursor 3 cDNA
Clone info. Amyloid precursor gene (include A4 region)
Vector backbone pUC119 (Plasmid)
Size of vector backbone 3.2 kb
Selectable markers Amp^r
Gene/insert name Human Amyloid (A4) cDNA
Depositor|Developer Imai, Takashi |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01067 pAMC3.0 DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAMC3.0 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01067).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12278_A3Jh.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12278_A3Jha.seq check
>01067_12278_A3Jh_M13(-40)_E01_13.ab1
    1 CCGCCGGGAT CAGACCAGCC TGGCCACATG GCAAAACCCT GTCTCTACTA AAAATACAAA
   61 AATTAGCCAG GCGTGGTGGT ATGCCCCTGT AATCCCAGTT ACTCAGGAGG CTGAGAGTGA
  121 GGTAGGAGAA TCGCTTGAAC CTGGGAGGTG GAGGTTGCAG TGAGCGGAGA TCATGCCACT
  181 GCACTCCAGC CTGGGTGAAA GAGCAAGACT CCATCTCAAA AAACAAAGAA ACAACAACAA
  241 CAAAAGAAAA CAACAAAACC ATGAAAAAAA CCAAAACAAC AACAGCAGCA AAAAAGGGGT
  301 CTTGAATTAC TCAAGTATGA AGACATCTTT ATTTGCTCAA ATATGGAATT AAATTTTTTT
  361 AAAAATTAAT TTGAGCAGAA ATGAAATAAG CTGATACTAA TGAAAACCTA TCTTACACTG
  421 TTTTGAGGGA CATAAGCTTA GATACTATCA CAAAAGGTGC AATGGCCAAA CATGACAGTG
  481 GGGTCATTGC TTTTATGTGC TCACACTCTG ATCTATGGGT CAGTCCGTTC CTTTCCACCC
  541 TGCTGCCTGC TGTTCTCTTG GTTTCCTTCC TCACACTGCT CATCCACGTC AGTATGAATG
  601 GGGAAATGAG GCTCCACCCC AATTTCCATC AGCAGCTTCT GTTGTAGTCT GCAGAGGTTT
661 TAATTGCTGA GCA
//
Primer:
Sequence file: RDB12278_A3Jhb.seq check
>01067_12278_A3Jh_Reverse2_F01_16.ab1
    1 AATACTCATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCGGGTACCG
   61 AGCTCGAATT CTGCAGAATT ACTATAGCTA AAGTAGGTAA CAAGATATCT TTTTTTCTAT
  121 TGTTTAACTC CTTTGTTTCA GAATGCCTAT TCCTGTGCAT TAAAAGTGTC CCTCCAAGGA
  181 AATTAGGACA TCTGCAGAGT TGAAAAACAC CTAAGTCTCA GTCACTTAGA GTCACACATC
  241 AGGGCTCAGA GTGCTATGAC TAGGAAAATG CTGACCTCCT TTCATTAGTA GGATCGTGCC
  301 TTTCCAGCTT TTGATAGATC CAAGCGCTAT CTTCCCACCA CTCACCAAAT GTTCCACCTG
  361 TCAAAGGGTT TCAGGTCCCT GCAGACTTCG GTTTTGACCT GTGAGGGGAA AGTAGACTTC
  421 CTCGAACTGG GGAAGCCACA TGTTGTACAT CCTTCTATAA ACTATGATTA TCATTCTTAG
  481 TAGGAAAATA TGTGATTTCT TTTTTTTTTT TTTTTTTAAA GTAAGCATCA AATATTTGAC
  541 CAACCAGTTG GGCAGAGAAT ATACTGAAAC TTTTTATATA ACCTCATCCA AATGTCCCCT
  601 GCATTTAAGA AATGAAATTC TTCTAATTGC GTTTATAAAT TGTAAATTAT ATTGCATTTA
661 GAAATTAAAA TTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Kang, J., The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature, 325, 733-736 (1987). PMID 2881207.