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pAMC3.0

Human amyloid precursor 3 cDNA

Catalog number RDB01067
Resource name pAMC3.0
Alternative name Human amyloid precursor 3 cDNA
Clone info. Amyloid precursor gene (include A4 region)
Vector backbone pUC119 (Plasmid)
Size of vector backbone 3.2 kb
Selectable markers Amp^r
Gene/insert name Human Amyloid (A4) cDNA
Depositor|Developer Imai, Takasi |
Other clones in our bank
External Database
Human Amyloid (A4)
          Reference sequence
            M15533, M15532
           
          Remarks, protocol and/or map (gif) RDB01067.gif

          Distribution information

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          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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          RDB01067 pAMC3.0 DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pAMC3.0 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01067).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          Tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12278_A3Jh.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB12278_A3Jha.seq check
          >01067_12278_A3Jh_M13(-40)_E01_13.ab1
              1 CCGCCGGGAT CAGACCAGCC TGGCCACATG GCAAAACCCT GTCTCTACTA AAAATACAAA
             61 AATTAGCCAG GCGTGGTGGT ATGCCCCTGT AATCCCAGTT ACTCAGGAGG CTGAGAGTGA
            121 GGTAGGAGAA TCGCTTGAAC CTGGGAGGTG GAGGTTGCAG TGAGCGGAGA TCATGCCACT
            181 GCACTCCAGC CTGGGTGAAA GAGCAAGACT CCATCTCAAA AAACAAAGAA ACAACAACAA
            241 CAAAAGAAAA CAACAAAACC ATGAAAAAAA CCAAAACAAC AACAGCAGCA AAAAAGGGGT
            301 CTTGAATTAC TCAAGTATGA AGACATCTTT ATTTGCTCAA ATATGGAATT AAATTTTTTT
            361 AAAAATTAAT TTGAGCAGAA ATGAAATAAG CTGATACTAA TGAAAACCTA TCTTACACTG
            421 TTTTGAGGGA CATAAGCTTA GATACTATCA CAAAAGGTGC AATGGCCAAA CATGACAGTG
            481 GGGTCATTGC TTTTATGTGC TCACACTCTG ATCTATGGGT CAGTCCGTTC CTTTCCACCC
            541 TGCTGCCTGC TGTTCTCTTG GTTTCCTTCC TCACACTGCT CATCCACGTC AGTATGAATG
            601 GGGAAATGAG GCTCCACCCC AATTTCCATC AGCAGCTTCT GTTGTAGTCT GCAGAGGTTT
          661 TAATTGCTGA GCA
          //
          Primer:
          Sequence file: RDB12278_A3Jhb.seq check
          >01067_12278_A3Jh_Reverse2_F01_16.ab1
              1 AATACTCATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCGGGTACCG
             61 AGCTCGAATT CTGCAGAATT ACTATAGCTA AAGTAGGTAA CAAGATATCT TTTTTTCTAT
            121 TGTTTAACTC CTTTGTTTCA GAATGCCTAT TCCTGTGCAT TAAAAGTGTC CCTCCAAGGA
            181 AATTAGGACA TCTGCAGAGT TGAAAAACAC CTAAGTCTCA GTCACTTAGA GTCACACATC
            241 AGGGCTCAGA GTGCTATGAC TAGGAAAATG CTGACCTCCT TTCATTAGTA GGATCGTGCC
            301 TTTCCAGCTT TTGATAGATC CAAGCGCTAT CTTCCCACCA CTCACCAAAT GTTCCACCTG
            361 TCAAAGGGTT TCAGGTCCCT GCAGACTTCG GTTTTGACCT GTGAGGGGAA AGTAGACTTC
            421 CTCGAACTGG GGAAGCCACA TGTTGTACAT CCTTCTATAA ACTATGATTA TCATTCTTAG
            481 TAGGAAAATA TGTGATTTCT TTTTTTTTTT TTTTTTTAAA GTAAGCATCA AATATTTGAC
            541 CAACCAGTTG GGCAGAGAAT ATACTGAAAC TTTTTATATA ACCTCATCCA AATGTCCCCT
            601 GCATTTAAGA AATGAAATTC TTCTAATTGC GTTTATAAAT TGTAAATTAT ATTGCATTTA
          661 GAAATTAAAA TTC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          reference Kang, J., The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature, 325, 733-736 (1987). PMID 2881207.

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl