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Akeo Shinkai's Resource

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Akeo Shinkai's web page in English   in Japanese.

Catalog #Name of cloneRunning titlePublication
RDB15775pUC-cmr6hPlasmid for introduction of (His)6-tag-coding sequence at the 3'-terminal of the cmr6 gene (TTHB165) with kanamycin resistance marker.Staals, R.H.J., Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus. Mol. Cell 2013 Oct 10;52(1):135-145 (2013). PMID 24119403. (original).
RDB15776pUC-csm5hPlasmid for introduction of (His)6-tag-coding sequence at the 3'-terminal of the csm5 gene (TTHB151) with kanamycin resistance marker.Staals, R.H., RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus. Mol. Cell 56 (4): 518-530 (2014). PMID 25457165. (original).
RDB15777PD014023-01Plasmid for gene disruption at TTHB023 locus by kanamycin resistance gene.Agari, Y., Transcriptional repression mediated by a TetR family protein, PfmR, from Thermus thermophilus HB8. J. Bacteriol. 194 (17): 4630-4641 (2012). PMID 22753056. (original).
RDB15778PC014023-41Bacterial (E. coli) expression plasmid clone of Thermus thermophilus HB8 TTHB023 (pfmR).Agari, Y., Transcriptional repression mediated by a TetR family protein, PfmR, from Thermus thermophilus HB8. J. Bacteriol. 194 (17): 4630-4641 (2012). PMID 22753056. (original).
RDB15779PC014162-42Bacterial (E. coli) expression plasmid clone of Thermus thermophilus HB8 TTHB162 (Cmr1).Staals, R.H.J., Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus. Mol. Cell 2013 Oct 10;52(1):135-145 (2013). PMID 24119403. (original).

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2018.12.11

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