Resource data sheet
Prev.

pUC-cmr6h

Plasmid for introduction of (His)6-tag-coding sequence at the 3'-terminal of the cmr6 gene (TTHB165) with kanamycin resistance marker.

Catalog number RDB15775
Resource name pUC-cmr6h
Clone info. Plasmid for introduction of (His)6-tag-coding sequence at the 3'-terminal of the cmr6 gene (TTHB165) with kanamycin resistance marker gene by means of homologus recombination on the genome of Thermus thermophilus HB8.
Host strains Stbl2 and Stbl3 are recommended. Expression was confirmed by the depositor with purification of Cmr complex using Ni-resin colum.
Vector backbone pUC19 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Thermus thermophilus HB8 TTHB165 genomic DNA Staphylococcus aureus kanamycin nucleotidyltransferase (HTK) genomic DNA Thermus thermophilus HB8 TTHB166 genomic DNA
Depositor Shinkai, Akeo |
 
Other clones in our bank Thermus thermophilus HB8 TTHB165 (KEGG orthology K19142) | Staphylococcus aureus (KEGG orthology K17882) | Thermus thermophilus HB8 TTHB166 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Staals RHJ, et al. Mol. Cell 52, 135-145, 2013)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15775 pUC-cmr6h Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Staals, R.H.J., Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus. Mol. Cell 2013 Oct 10;52(1):135-145 (2013). PMID 24119403.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Staals, R.H.J., Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus. Mol. Cell 2013 Oct 10;52(1):135-145 (2013). PMID 24119403.

2019.07.17

GNP_filter3_RDBDEP_html_190432.pl