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pUC-csm5h (#RDB15776)

Plasmid for introduction of (His)6-tag-coding sequence at the 3'-terminal of the csm5 gene (TTHB151) with kanamycin resistance marker.

Clone info. Plasmid for introduction of (His)6-tag-coding sequence at the 3'-terminal of the csm5 gene (TTHB151) with kanamycin resistance marker gene by means of homologus recombinationon the genome of Thermus thermophilus HB8.
Comment Host strains Stbl2 and Stbl3 are recommended. Expression was confirmed by the depositor with purification of Csm complex using Ni-resin colum.
Vector backbone pUC19 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r (E. coli)
Gene/insert name Thermus thermophilus HB8 TTHB151 genomic DNA Staphylococcus aureus kanamycin nucleotidyltransferase (HTK) genomic DNA Thermus thermophilus HB8 TTHB152 genomic DNA
Depositor|Developer Shinkai, Akeo |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Staals RHJ, et al. Mol. Cell 56, 518-530, 2014)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Staals RHJ, et al. Mol. Cell 56, 518-530, 2014)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15776 pUC-csm5h Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pUC-csm5h was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15776).

Reference section:

Staals, R.H., Zhu, Y., Taylor, D.W., Kornfeld, J.E., Sharma, K., Barendregt, A., Koehorst, J.J., Vlot, M., Neupane, N., Varossieau, K., Sakamoto, K., Suzuki, T., Dohmae, N., Yokoyama, S., Schaap, P.J., Urlaub, H., Heck, A.J., Nogales, E., Doudna, J.A., Shinkai, A., van der Oost, J., RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus. Mol. Cell 56 (4): 518-530 (2014). PMID 25457165. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Staals, R.H., RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus. Mol. Cell 56 (4): 518-530 (2014). PMID 25457165. [link to RRC of NBRP]