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pEGFP-C1-EVI5-R208K (#RDB20830)

Expression vector of human EVI5 GAP-activity-deficient mutant (R208K) tagged with EGFP at N-terminus


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Nakashima, S., Traffic 26 (4-6): e70007 (2025). PMID 40241313. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human EVI5 GAP-activity-deficient mutant (R208K) tagged with EGFP at N-terminus. Originated from pEGFP-C1-human EVI5 (RDB15021).
Comment Difference compared with CDS of XM_024449686.2 : 108 C > T (Asn, synonymous), 573 C > T (Leu, synonymous), 734 A > G (Q245R), 2004 G > A (Val, synonymous), 2135 - 2136 ins AGTCCACATCAGC (13 nt; frameshift).
Vector backbone pEGFP-C1 (plasmid)
Growth conditions LB+KM, 37℃
Gene/insert name human EVI5 cDNA.
Depositor|Developer Fukuda, Mitsunori |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Nakashima, S. and Fukuda, M. Traffic 26 (4-6): e70007 (2025)) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Tohoku University.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakashima, S. and Fukuda, M. Traffic 26 (4-6): e70007 (2025))。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、東北大学の許可を得ること。

Catalog # Resource name Shipping form Fee
RDB20830 pEGFP-C1-EVI5-R208K DNA solution

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-EVI5-R208K was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20830).

Reference section:

Nakashima, S., Fukuda, M, Identification of Rab GTPase-Activating Proteins Required for Tubular Endosome Formation. Traffic 26 (4-6): e70007 (2025). PMID 40241313. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakashima, S., Identification of Rab GTPase-Activating Proteins Required for Tubular Endosome Formation. Traffic 26 (4-6): e70007 (2025). PMID 40241313. [PubMed] [Article] [RRC of NBRP]