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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-T7-GST (#RDB19882)

Empty backbone for expression of T7-GST tag protein, EF1 promoter.

Clone info. Empty backbone for expression of T7-GST tag protein, EF1 promoter. A derivative of pEF-T7 (Fukuda, M. et al. J. Biol. Chem., 274 (44): 31421-31427, 1999. PMID 10531343).
Vector backbone pEF-BOS (plasmid)
Selectable markers Ampicillin
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Fukuda et al. J. Biol. Chem. 227, 12432-12436, 2002)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda et al. J. Biol. Chem. 227, 12432-12436, 2002)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19882 pEF-T7-GST DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-GST was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19882).

Reference section:

Fukuda, M., Kuroda, T.S., Mikoshiba, K., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19882_B3B3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,insert 5'(T7tag,GST 5')
Sequence file: RDB19882_B3B3a.seq check
>D09174A1_B3B3_3_EF1a-C_F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNTTTNNAN TTTGGNNCTT GGTTNNTTCT CNAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGCGGAT CTATGTCCCC
  181 TATACTAGGT TATTGGAAAA TTAAGGGCCT TGTGCAACCC ACTCGACTTC TTTTGGAATA
  241 TCTTGAAGAA AAATATGAAG AGCATTTGTA TGAGCGCGAT GAAGGTGATA AATGGCGAAA
  301 CAAAAAGTTT GAATTGGGTT TGGAGTTTCC CAATCTTCCT TATTATATTG ATGGTGATGT
  361 TAAATTAACA CAGTCTATGG CCATCATACG TTATATAGCT GACAAGCACA ACATGTTGGG
  421 TGGTTGTCCA AAAGAGCGTG CAGAGATTTC AATGCTTGAA GGAGCGGTTT TGGATATTAG
  481 ATACGGTGTT TCGAGAATTG CATATAGTAA AGACTTTGAA ACTCTCAAAG TTGATTTTCT
  541 TAGCAAGCTA CCTGAAATGC TGAAAATGTT CGAAGATCGT TTATGTCATA AAACATATTT
  601 AAATGGTGAT CATGTAACCC ATCCTGACTT CATGTTGTAT GACGCTCTTG ATGTTGTTTT
  661 ATACATGGAC CCNATGTGCC TGGATGCGTT CCCAAAATTA GTTTGTTTTA AAAAACGTAT
  721 TGAAGCTATC CCACAAATTG ATAAGTACTT GAANTCCAGC AAGTATATAG CATGGCCTTT
  781 GCANGGCTGG CAAGCCACGT TTGGTGGTGG CGANCATCCT NNAAAATCGG ATCTGGNNCC
  841 GCGNGNATCC NNNCTAATTA NCGGNCGCTC TAGANNGANG GNCCCCNNCN GGGNNCCNNN
  901 ANANTATCNN NNNNCCNNCN NNGNANANNN NNNNCCNNNN TNANTANTTN AANNNNNANN
961 NNNNCCNNNN NNGNNNNNNN NNN
//
Primer: hGCSF_R (Pr0031)
Region: hGCSF 3' UTR+pA,insert(thrombin site, GST,T7 tag),EF-1 alpha pro
Sequence file: RDB19882_B3B3b.seq check
>D09174A1_B3B3_3_hGCSF_R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNAGGN TCCNNGGTGG GGNNCCTCAC TCTAGAGCGG CCGCTAATTA
   61 GTTAGGATCC ACGCGGAACC AGATCCGATT TTGGAGGATG GTCGCCACCA CCAAACGTGG
  121 CTTGCCAGCC CTGCAAAGGC CATGCTATAT ACTTGCTGGA TTTCAAGTAC TTATCAATTT
  181 GTGGGATAGC TTCAATACGT TTTTTAAAAC AAACTAATTT TGGGAACGCA TCCAGGCACA
  241 TTGGGTCCAT GTATAAAACA ACATCAAGAG CGTCATACAA CATGAAGTCA GGATGGGTTA
  301 CATGATCACC ATTTAAATAT GTTTTATGAC ATAAACGATC TTCGAACATT TTCAGCATTT
  361 CAGGTAGCTT GCTAAGAAAA TCAACTTTGA GAGTTTCAAA GTCTTTACTA TATGCAATTC
  421 TCGAAACACC GTATCTAATA TCCAAAACCG CTCCTTCAAG CATTGAAATC TCTGCACGCT
  481 CTTTTGGACA ACCACCCAAC ATGTTGTGCT TGTCAGCTAT ATAACGTATG ATGGCCATAG
  541 ACTGTGTTAA TTTAACATCA CCATCAATAT AATAAGGAAG ATTGGGAAAC TCCAAACCCA
  601 ATTCAAACTT TTTGTTTCGC CATTTATCAC CTTCATCGCG CTCATACAAA TGCTCTTCAT
  661 ATTTTTCTTC AAGATATTCC AAAAGAAGTC GAGTGGGTTG CACAAGGCCC TTAATTTTCC
  721 AATAACCTAG TATAGGGGAC ATAGATCCGC GACCCATTTG CTGTCCACCA GTCATGCTAG
  781 CCATGGTGGA ATCGAATTCC CGCGGCCGCT CTAGAGAATT CCTCACGACA CCTGAAATGG
  841 AAGAAAAAAA CTTTGNNCCA CNGTCTGANN CTTGAGAATG ANNNNAGNNN CAAACTCAAA
  901 AAGGGNAAAT TNCAAGGANA ATTANNTCNA GNGNNNNGCN NGNNNNNNNT TCANNCTNNN
961 NCNNCTCNNN GNGGGGNANN TNCNNNNNAN NAANNCNNNN NNNNNN
//
Primer: SV40pro_F_V3 (Pr1252)
Region: EF-1 alpha pro
Sequence file: RDB19882_B3B3c.seq check
>D09174A1_B3B3_3_SV40pro_F_V3_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNTTNNNNGC NNNGGNNCTT CTAGGTCTTG NAAGGAGTGG GAATTGGCTC
   61 CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG
  121 GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT
  181 CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT
  241 CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA GTGCCGTGTG
  301 TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT GAATTACTTC
  361 CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG GGTGGGAGAG
  421 TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG
  481 GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA
  541 TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA
  601 TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG
  661 CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG
  721 GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT
  781 CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GNCCGGTCGG CACCAGTTGC
  841 GTGANCGGAA AGANGGNCGC TTCCCGGNCC TGCTGCNNGN NNCTCNAAAT GGANGANGCG
  901 GNGCTCGGGA NANCGGGCGG GNNANTCACC CACACAAAGG AAANNNGNCT TTNNNNNNNC
961 ANNNNNCNCT TCNTGNNANT NNNNNNNNNN
//
Primer: EF1a-M_F3 (Pr1242)
Region: EF-1 alpha pro
Sequence file: RDB19882_B3B3d.seq check
>D09174A1_B3B3_3_EF1a-M_F3_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNC NNGCTTGANT TGANGCCTGG CCTGGGCGCT GGGGCCGCCG
   61 CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT
  121 TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC
  181 GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG
  241 TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG
  301 ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT
  361 CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG
  421 GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG
  481 GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG
  541 TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG
  601 TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG
  661 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT
  721 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT
  781 TCCATTTCAN GTGTCGTGAG GAATTCTCTA GAGCGGCCGC GGGAATTCGA TTCCACCATG
  841 GCTAGCATGA CTGGTGGACA GCAAATGGGT CGCGGNTCTA TGTNCCNTAT ACNANGTTAN
901 TGNAAAATTA ANGNNCNNNN NCNNNCCACN CNNCTTCNTT NNGNNNNNNN NNN
//
Primer: GST-N_rev (Pr0448)
Region: insert 5'(GST 5',T7 tag),EF-1 alpha pro
Sequence file: RDB19882_B3B3e.seq check
>D09174A1_B3B3_3_GST-N_rev_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCGAGTGG GTTGCACAAG GCCCTTAATT TTCCNATAAC
   61 CTAGTATAGG GGACATAGAT CCGCGACCCA TTTGCTGTCC ACCAGTCATG CTAGCCATGG
  121 TGGAATCGAA TTCCCGCGGC CGCTCTAGAG AATTCCTCAC GACACCTGAA ATGGAAGAAA
  181 AAAACTTTGA ACCACTGTCT GAGGCTTGAG AATGAACCAA GATCCAAACT CAAAAAGGGC
  241 AAATTCCAAG GAGAATTACA TCAAGTGCCA AGCTGGCCTA ACTTCAGTCT CCACCCACTC
  301 AGTGTGGGGA AACTCCATCG CATAAAACCC CTCCCCCCAA CCTAAAGACG ACGTACTCCA
  361 AAAGCTCGAG AACTAATCGA GGTGCCTGGA CGGCGCCCGG TACTCCGTGG AGTCACATGA
  421 AGCGACGGCT GAGGACGGAA AGGCCCTTTT CCTTTGTGTG GGTGACTCAC CCGCCCGCTC
  481 TCCCGAGCGC CGCGTCCTCC ATTTTGAGCT CCCTGCAGCA GGGCCGGGAA GCGGCCATCT
  541 TTCCGCTCAC GCAACTGGTG CCGACCGGGC CAGCCTTGCC GCCCAGGGCG GGGCGATACA
  601 CGGCGGCGCG AGGCCAGGCA CCAGAGCAGG CCGGCCAGCT TGAGACTACC CCCGTCCGAT
  661 TCTCGGTGGC CGCGCTCGCA GGCCCCGCCT CGCCGAACAT GTGCGCTGGG ACGCACGGGC
  721 CCCGTCGCCG CCCGCGGCCC CAAAAACCGA ANTACCAGTG TGCAGATCTT GGCCCGCATT
  781 TACAAGANTA TCTTGCCAGA AAAAAAGCGT CGCANCANGT CATCAAAAAT TTTAAATGNN
  841 TAGAGACTTA TCGAAAGCAG CGAGACANGN GCGAANGNGC CNNCAGANTC GCACGCGGCG
  901 GCCCCAGCGC CNNGGCCAGG NNNNNACTCN NGCNCGANNC NNANNGGNCT NNNNNNNNNN
//
Primer: pGEX-F2 (Pr0403)
Region: insert 3'(GST 3',thrombin site),hGCSF 3' UTR+pA
Sequence file: RDB19882_B3B3f.seq check
>D09174A1_B3B3_3_pGEX-F2_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGTATTG AAGCTATCCC ACAAATTGAT AAGTACTTGA
   61 AATCCAGCAA GTATATAGCA TGGCCTTTGC AGGGCTGGCA AGCCACGTTT GGTGGTGGCG
  121 ACCATCCTCC AAAATCGGAT CTGGTTCCGC GTGGATCCTA ACTAATTAGC GGCCGCTCTA
  181 GAGTGAGGGT CCCCACCTGG GACCCTTGAG AGTATCAGGT CTCCCACGTG GGAGACAAGA
  241 AATCCCTGTT TAATATTTAA ACAGCAGTGT TCCCCATCTG GGTCCTTGCA CCCCTCACTC
  301 TGGCCTCAGC CGACTGCACA GCGGCCCCTG CATCCCCTTG GCTGTGAGGC CCCTGGACAA
  361 GCAGAGGTGG CCAGAGCTGG GAGGCATGGC CCTGGGGTCC CACGAATTTG CTGGGGAATC
  421 TCGTTTTTCT TCTTAAGACT TTTGGGACAT GGTTTGACTC CCGAACATCA CCGACGTGTC
  481 TCCTGTTTTT CTGGGTGGCC TCGGGACACC TGCCCTGCCC CCACGAGGGT CAGGACTGTG
  541 ACTCTTTTTA GGGCCAGGCA GGTGCCTGGA CATTTGCCTT GCTGGACGGG GACTGGGGAT
  601 GTGGGAGGGA GCAGACAGGA GGAATCATGT CAGGCCTGTG TGTGAAAGGA AGCTCCACTG
  661 TCACCCTCCA CCTCTTCACC CCCCACTCAC CAGTGTCCCC TCCACTGTCA CATTGTAACT
721 GAACTTCNNG ATAATAAAGT GTTTGCCTCN NNNAAAANNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [link to RRC of NBRP]