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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-T7-GST

Empty backbone for expression of T7-GST tag protein, EF1 promoter.

Catalog number RDB19882
Resource name pEF-T7-GST
Clone info. Empty backbone for expression of T7-GST tag protein, EF1 promoter. A derivative of pEF-T7 (Fukuda, M. et al. J. Biol. Chem., 274 (44): 31421-31427, 1999. PMID 10531343).
Vector backbone pEF-BOS (plasmid)
Selectable markers Ampicillin
Depositor|Developer Fukuda, Mitsunori |
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External Database

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Fukuda et al. J. Biol. Chem. 227, 12432-12436, 2002)
            Additional terms and conditions:
            Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda et al. J. Biol. Chem. 227, 12432-12436, 2002)。
            付加的提供条件:
            pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19882 pEF-T7-GST DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pEF-T7-GST was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19882).

            Reference section:

            Fukuda, M., Kuroda, T.S., Mikoshiba, K., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.

            Further references such as user reports and related articles (go to bottom)


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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19882_B3B3p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F (Pr0168)
            Region: EF-1 alpha pro,insert 5'(T7tag,GST 5')
            Sequence file: RDB19882_B3B3a.seq check
            >D09174A1_B3B3_3_EF1a-C_F_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNNTTTNNAN TTTGGNNCTT GGTTNNTTCT CNAGCCTCAG ACAGTGGTTC
               61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
              121 CGATTCCACC ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGCGGAT CTATGTCCCC
              181 TATACTAGGT TATTGGAAAA TTAAGGGCCT TGTGCAACCC ACTCGACTTC TTTTGGAATA
              241 TCTTGAAGAA AAATATGAAG AGCATTTGTA TGAGCGCGAT GAAGGTGATA AATGGCGAAA
              301 CAAAAAGTTT GAATTGGGTT TGGAGTTTCC CAATCTTCCT TATTATATTG ATGGTGATGT
              361 TAAATTAACA CAGTCTATGG CCATCATACG TTATATAGCT GACAAGCACA ACATGTTGGG
              421 TGGTTGTCCA AAAGAGCGTG CAGAGATTTC AATGCTTGAA GGAGCGGTTT TGGATATTAG
              481 ATACGGTGTT TCGAGAATTG CATATAGTAA AGACTTTGAA ACTCTCAAAG TTGATTTTCT
              541 TAGCAAGCTA CCTGAAATGC TGAAAATGTT CGAAGATCGT TTATGTCATA AAACATATTT
              601 AAATGGTGAT CATGTAACCC ATCCTGACTT CATGTTGTAT GACGCTCTTG ATGTTGTTTT
              661 ATACATGGAC CCNATGTGCC TGGATGCGTT CCCAAAATTA GTTTGTTTTA AAAAACGTAT
              721 TGAAGCTATC CCACAAATTG ATAAGTACTT GAANTCCAGC AAGTATATAG CATGGCCTTT
              781 GCANGGCTGG CAAGCCACGT TTGGTGGTGG CGANCATCCT NNAAAATCGG ATCTGGNNCC
              841 GCGNGNATCC NNNCTAATTA NCGGNCGCTC TAGANNGANG GNCCCCNNCN GGGNNCCNNN
              901 ANANTATCNN NNNNCCNNCN NNGNANANNN NNNNCCNNNN TNANTANTTN AANNNNNANN
            961 NNNNCCNNNN NNGNNNNNNN NNN
            //
            Primer: hGCSF_R (Pr0031)
            Region: hGCSF 3' UTR+pA,insert(thrombin site, GST,T7 tag),EF-1 alpha pro
            Sequence file: RDB19882_B3B3b.seq check
            >D09174A1_B3B3_3_hGCSF_R_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNAGGN TCCNNGGTGG GGNNCCTCAC TCTAGAGCGG CCGCTAATTA
               61 GTTAGGATCC ACGCGGAACC AGATCCGATT TTGGAGGATG GTCGCCACCA CCAAACGTGG
              121 CTTGCCAGCC CTGCAAAGGC CATGCTATAT ACTTGCTGGA TTTCAAGTAC TTATCAATTT
              181 GTGGGATAGC TTCAATACGT TTTTTAAAAC AAACTAATTT TGGGAACGCA TCCAGGCACA
              241 TTGGGTCCAT GTATAAAACA ACATCAAGAG CGTCATACAA CATGAAGTCA GGATGGGTTA
              301 CATGATCACC ATTTAAATAT GTTTTATGAC ATAAACGATC TTCGAACATT TTCAGCATTT
              361 CAGGTAGCTT GCTAAGAAAA TCAACTTTGA GAGTTTCAAA GTCTTTACTA TATGCAATTC
              421 TCGAAACACC GTATCTAATA TCCAAAACCG CTCCTTCAAG CATTGAAATC TCTGCACGCT
              481 CTTTTGGACA ACCACCCAAC ATGTTGTGCT TGTCAGCTAT ATAACGTATG ATGGCCATAG
              541 ACTGTGTTAA TTTAACATCA CCATCAATAT AATAAGGAAG ATTGGGAAAC TCCAAACCCA
              601 ATTCAAACTT TTTGTTTCGC CATTTATCAC CTTCATCGCG CTCATACAAA TGCTCTTCAT
              661 ATTTTTCTTC AAGATATTCC AAAAGAAGTC GAGTGGGTTG CACAAGGCCC TTAATTTTCC
              721 AATAACCTAG TATAGGGGAC ATAGATCCGC GACCCATTTG CTGTCCACCA GTCATGCTAG
              781 CCATGGTGGA ATCGAATTCC CGCGGCCGCT CTAGAGAATT CCTCACGACA CCTGAAATGG
              841 AAGAAAAAAA CTTTGNNCCA CNGTCTGANN CTTGAGAATG ANNNNAGNNN CAAACTCAAA
              901 AAGGGNAAAT TNCAAGGANA ATTANNTCNA GNGNNNNGCN NGNNNNNNNT TCANNCTNNN
            961 NCNNCTCNNN GNGGGGNANN TNCNNNNNAN NAANNCNNNN NNNNNN
            //
            Primer: SV40pro_F_V3 (Pr1252)
            Region: EF-1 alpha pro
            Sequence file: RDB19882_B3B3c.seq check
            >D09174A1_B3B3_3_SV40pro_F_V3_C03_09_ABI24.ab1
                1 NNNNNNNNNN NNTTNNNNGC NNNGGNNCTT CTAGGTCTTG NAAGGAGTGG GAATTGGCTC
               61 CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG
              121 GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT
              181 CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT
              241 CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA GTGCCGTGTG
              301 TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT GAATTACTTC
              361 CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG GGTGGGAGAG
              421 TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG
              481 GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA
              541 TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA
              601 TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG
              661 CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG
              721 GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT
              781 CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GNCCGGTCGG CACCAGTTGC
              841 GTGANCGGAA AGANGGNCGC TTCCCGGNCC TGCTGCNNGN NNCTCNAAAT GGANGANGCG
              901 GNGCTCGGGA NANCGGGCGG GNNANTCACC CACACAAAGG AAANNNGNCT TTNNNNNNNC
            961 ANNNNNCNCT TCNTGNNANT NNNNNNNNNN
            //
            Primer: EF1a-M_F3 (Pr1242)
            Region: EF-1 alpha pro
            Sequence file: RDB19882_B3B3d.seq check
            >D09174A1_B3B3_3_EF1a-M_F3_D03_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNCNNC NNGCTTGANT TGANGCCTGG CCTGGGCGCT GGGGCCGCCG
               61 CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT
              121 TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC
              181 GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG
              241 TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG
              301 ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT
              361 CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG
              421 GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG
              481 GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG
              541 TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG
              601 TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG
              661 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT
              721 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT
              781 TCCATTTCAN GTGTCGTGAG GAATTCTCTA GAGCGGCCGC GGGAATTCGA TTCCACCATG
              841 GCTAGCATGA CTGGTGGACA GCAAATGGGT CGCGGNTCTA TGTNCCNTAT ACNANGTTAN
            901 TGNAAAATTA ANGNNCNNNN NCNNNCCACN CNNCTTCNTT NNGNNNNNNN NNN
            //
            Primer: GST-N_rev (Pr0448)
            Region: insert 5'(GST 5',T7 tag),EF-1 alpha pro
            Sequence file: RDB19882_B3B3e.seq check
            >D09174A1_B3B3_3_GST-N_rev_E03_15_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNTCGAGTGG GTTGCACAAG GCCCTTAATT TTCCNATAAC
               61 CTAGTATAGG GGACATAGAT CCGCGACCCA TTTGCTGTCC ACCAGTCATG CTAGCCATGG
              121 TGGAATCGAA TTCCCGCGGC CGCTCTAGAG AATTCCTCAC GACACCTGAA ATGGAAGAAA
              181 AAAACTTTGA ACCACTGTCT GAGGCTTGAG AATGAACCAA GATCCAAACT CAAAAAGGGC
              241 AAATTCCAAG GAGAATTACA TCAAGTGCCA AGCTGGCCTA ACTTCAGTCT CCACCCACTC
              301 AGTGTGGGGA AACTCCATCG CATAAAACCC CTCCCCCCAA CCTAAAGACG ACGTACTCCA
              361 AAAGCTCGAG AACTAATCGA GGTGCCTGGA CGGCGCCCGG TACTCCGTGG AGTCACATGA
              421 AGCGACGGCT GAGGACGGAA AGGCCCTTTT CCTTTGTGTG GGTGACTCAC CCGCCCGCTC
              481 TCCCGAGCGC CGCGTCCTCC ATTTTGAGCT CCCTGCAGCA GGGCCGGGAA GCGGCCATCT
              541 TTCCGCTCAC GCAACTGGTG CCGACCGGGC CAGCCTTGCC GCCCAGGGCG GGGCGATACA
              601 CGGCGGCGCG AGGCCAGGCA CCAGAGCAGG CCGGCCAGCT TGAGACTACC CCCGTCCGAT
              661 TCTCGGTGGC CGCGCTCGCA GGCCCCGCCT CGCCGAACAT GTGCGCTGGG ACGCACGGGC
              721 CCCGTCGCCG CCCGCGGCCC CAAAAACCGA ANTACCAGTG TGCAGATCTT GGCCCGCATT
              781 TACAAGANTA TCTTGCCAGA AAAAAAGCGT CGCANCANGT CATCAAAAAT TTTAAATGNN
              841 TAGAGACTTA TCGAAAGCAG CGAGACANGN GCGAANGNGC CNNCAGANTC GCACGCGGCG
              901 GCCCCAGCGC CNNGGCCAGG NNNNNACTCN NGCNCGANNC NNANNGGNCT NNNNNNNNNN
            //
            Primer: pGEX-F2 (Pr0403)
            Region: insert 3'(GST 3',thrombin site),hGCSF 3' UTR+pA
            Sequence file: RDB19882_B3B3f.seq check
            >D09174A1_B3B3_3_pGEX-F2_F06_18_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNGTATTG AAGCTATCCC ACAAATTGAT AAGTACTTGA
               61 AATCCAGCAA GTATATAGCA TGGCCTTTGC AGGGCTGGCA AGCCACGTTT GGTGGTGGCG
              121 ACCATCCTCC AAAATCGGAT CTGGTTCCGC GTGGATCCTA ACTAATTAGC GGCCGCTCTA
              181 GAGTGAGGGT CCCCACCTGG GACCCTTGAG AGTATCAGGT CTCCCACGTG GGAGACAAGA
              241 AATCCCTGTT TAATATTTAA ACAGCAGTGT TCCCCATCTG GGTCCTTGCA CCCCTCACTC
              301 TGGCCTCAGC CGACTGCACA GCGGCCCCTG CATCCCCTTG GCTGTGAGGC CCCTGGACAA
              361 GCAGAGGTGG CCAGAGCTGG GAGGCATGGC CCTGGGGTCC CACGAATTTG CTGGGGAATC
              421 TCGTTTTTCT TCTTAAGACT TTTGGGACAT GGTTTGACTC CCGAACATCA CCGACGTGTC
              481 TCCTGTTTTT CTGGGTGGCC TCGGGACACC TGCCCTGCCC CCACGAGGGT CAGGACTGTG
              541 ACTCTTTTTA GGGCCAGGCA GGTGCCTGGA CATTTGCCTT GCTGGACGGG GACTGGGGAT
              601 GTGGGAGGGA GCAGACAGGA GGAATCATGT CAGGCCTGTG TGTGAAAGGA AGCTCCACTG
              661 TCACCCTCCA CCTCTTCACC CCCCACTCAC CAGTGTCCCC TCCACTGTCA CATTGTAACT
            721 GAACTTCNNG ATAATAAAGT GTTTGCCTCN NNNAAAANNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.

            2023.05.11

            GNP_filter3_RDBDEP_html_230501.pl