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pmStr-C1-Vps9d1-Y628A (YA) (#RDB19826)

Expression vector of mouse Vps9d1-Y628A (GEF-activitiy-deficient mutant) tagged with mnonomeric Strawberry at N-terminus.


Drawn by SnapGene® software
Sequence (full) RDB19826hts01.seq
Publication Nakashima, S., J. Cell Sci. 136 (6): jcs260522 (2023). [link to RRC of NBRP]
Test sheet RDB19826_B3Ajp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of mouse Vps9d1-Y628A (GEF-activitiy-deficient mutant) tagged with mnonomeric Strawberry at N-terminus. See Fig. 2C of Nakashima et al. (2023) J. Cell Sci., 136, jcs260522.
Comment Expression was confirmed by the depositor with Westerrn blotting.
Vector backbone pmStr-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name mouse Vps9d1 cDNA.
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakashima, S., Matsui, T. and Fukuda, M. J. Cell Sci. 136, 260522, 2023)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakashima, S., Matsui, T. and Fukuda, M. J. Cell Sci. 136, 260522, 2023)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19826 pmStr-C1-Vps9d1-Y628A (YA) DNA solution

How to cite this biological resource

Materials & Methods section:

The pmStr-C1-Vps9d1-Y628A (YA) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19826).

Reference section:

Nakashima, S., Matsui, T., Fukuda, M., Vps9d1 regulates tubular endosome formation through specific activation of Rab22A. J. Cell Sci. 136 (6): jcs260522 (2023). PMID 36762583. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakashima, S., Vps9d1 regulates tubular endosome formation through specific activation of Rab22A. J. Cell Sci. 136 (6): jcs260522 (2023). PMID 36762583. [link to RRC of NBRP]