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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-hRUTBC3-R165A (#RDB19704)

Retroviral expression vector of human RUTBC3 GAP activity-deficient mutant tagged with EGFP at N-terminus.

Clone info. Retroviral expression vector of human RUTBC3 (RUN and TBC1 domain-containing protein 3) GAP activity-deficient R165A mutant tagged with EGFP at N-terminus. See Fig.3A of Hiragi, S. et al. (2022) J. Cell Biol. 221, e202201114
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human SGSM3 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB19704zzt01.seq compiled by us

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19704 pEGFP-C1-hRUTBC3-R165A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-hRUTBC3-R165A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19704).

Reference section:

Hiragi, S., Matsui, T., Sakamaki, Y., Fukuda, M., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19704_B2Jjp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB19704_B2Jja.seq check
>D09032A1_B2Jj_1_EGFP-C2_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNCGNNC NCNTGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GNNNAGCTGT ACAAGTCCGG ACTCAGATCC ATGTCAGGAA GCCATACACC
  121 TGCCTGTGGC CCTTTCTCAG CCCTGACTCC GAGCATATGG CCCCAGGAGA TCTTGGCCAA
  181 GTACACGCAG AAGGAAGAGT CAGCAGAGCA ACCAGAGTTC TACTACGATG AGTTTGGTTT
  241 CCGTGTGTAC AAGGAAGAAG GTGATGAGCC TGGCTCCAGT CTGCTGGCGA ACTCCCCTCT
  301 GATGGAGGAT GCTCCACAGA GGCTGCGGTG GCAGGCCCAC CTGGAGTTCA CCCATAACCA
  361 CGATGTGGGG GATCTCACCT GGGACAAGAT TGCCGTCTCC CTACCCCGCT CTGAGAAGCT
  421 CCGCTCCCTG GTGCTGGCCG GCATCCCACA TGGCATGAGG CCACAGCTGT GGATGCGGCT
  481 CTCTGGGGCC CTGCAGAAGA AGAGGAACTC TGAGCTGTCC TACCGCGAGA TTGTGAAGAA
  541 CAGCTCCAAC GATGAGACCA TCGCTGCCAA GCAGATCGAG AAGGACCTGC TCGCCACCAT
  601 GCCCAGCAAC GCCTGCTTCG CCAGCATGGG TAGCATCGGG GTACCCCGCC TGCGCAGGGT
  661 GCTCCGGGCC CTGGCCTGGC TCTACCCAGA GATCGGCTAC TGCCAGGGCA CCGGCATGGT
  721 GGCCGCCTGC CTCCTGCTGT TCCTGGAGGA NGANGANGCC TTCTGGATGA NGTCTGCCAT
  781 CATCGAGGAC CTGCTCCCCG CCTCCTACTT CAGCACCACC CTGCTGGGTG TCCAGACTGA
  841 NCAGCGGGTC CTGCGCCACC TCATTGTCCA GTATCTGCCT CGCCNGGACA AGCTGCTCCA
  901 GGANCATGAC ATTNAGCTGT CCCNGATCAC ACTGCACTGG TTNCTCACNG CCTTCNCNNG
  961 CGNGGNNGAN NTCAAGCTGC TNCNGCNCNT NNGGNNNCNG TTTTTCNNCN ANGGNTNCNN
1021 GGNNCTNNNN NNNCNNANNN NNGNNNNNNN NNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB19704_B2Jjb.seq check
>D09032A1_B2Jj_1_SV40polyA_R2_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGTTAA CAACNACAAT TGCATTCATT TTATGTTTCA
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT
  181 GCAGGCGGCC GCTATGGTCG ACTGCAGAAT TCGATTTCAC CCGTCCACAT CCCAGCTGAA
  241 GAGGTGGTGC TTCACCAGCA TGTCCCGGAC GCCCTCCTTC AGGGGCTGCT GCGCCTCTCT
  301 CTTCGCAGGG AGCTCCCAGT CCTGGGAGAG GCTGAAGGCA AAGCAGCAGA GGACTCGGAG
  361 CTCACACTTG ATCTGGACCC AGCCCGGGCT GCGCAGGAAG GACCAGGGCT GGTACCACTT
  421 CTCCACGGTG GGCAGGCTGG AGCAGAGCAC CTCCAGCCAC AGGTGCAGCA CCTGCTCATT
  481 GAGCCCCACG CAGATCAGTG AGCGGAGCTT CACATCCATT TGTGCATGCA CTGCATCGTG
  541 GGTCACGTTC ACAGACTGCA CAGCCCGGTA GAGCAGCTCC TCCGGGGTCA GGACTTTGCC
  601 ATCTTCATCC AACCTGAAGG TCTTACAGAG CACCAGACGG GAATACACGG AGGCAAAGTC
  661 TCTCTCGACC TCCCGGCCTG CAGCCTCCTC GATAAACAGC CAGGGGTGGC AGGCGCCCCC
  721 AAGCAGGGAT GGCTTCTTCA GTCCATGTTC GAACAGGGCC TTAANGGCCG GGCAGAGGGT
  781 CCCTCGCACG ANGTCTGTGA CCCCCTCCGT CACCGAGTCA TCCCCCGCGA TGGAGTACTC
  841 TTTGCTGCGC TCATCCAGGA NTTCCACGAA CTTGGCTGGA AANCAGCCTC GCNNNCGNTG
  901 ANCTCCCCCA CCCANCAGTG CTCGTNCTTC TGANACACGA TTGTGATGAT GTCNTTCTTG
961 CNGANNCCCA GCTCGTCGTC GTCNNGNCNC NCAAAGTCCA GCNNNNNNNN NN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB19704_B2Jjc.seq check
>D09032A1_B2Jj_1_CMV_Forward_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNNNNAAT GGGCGGTAGG CGTGTACGGT GGGNNGTCTA
   61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG
  121 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
  181 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
  241 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
  301 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG
  361 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
  421 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
  481 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
  541 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
  601 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
  661 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
  721 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
  781 CTGGAGTTCG TGACCGCCGC CNGGATCACT CTCGGCATGG ACGANCTGTA CAAGTCCGGA
  841 CTCAGATCCA TGTCANGAAG CCATACACCT GCCTGTGGCC CTTTCTCAGC CCTGACTCCG
  901 ANCATATGGN CCCAGGANAT CTTGNCCAAG TACACGCAGA ANGAANANTC ANCNNANCAN
  961 CNANNNTTCT ANTACNATNA NTTTGNTTTC CGNGNGNNNN NNNAANNANN NNGNNNANCN
1021 NNNNNNCNNN NNNNNNN
//
Primer: VN_F1 (Pr0992)
Region: EGFP 3',insert 5'
Sequence file: RDB19704_B2Jjd.seq check
>D09032A1_B2Jj_1_VN_F1_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTC TATATCATGG CCGANAAGCA GAAGAACGGC
   61 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
  121 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
  181 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
  241 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA
  301 CTCAGATCCA TGTCAGGAAG CCATACACCT GCCTGTGGCC CTTTCTCAGC CCTGACTCCG
  361 AGCATATGGN CNNNNGAGAT CTTGGCCAAG TACACGCAGA AGGAAGAGTC AGCAGAGCAA
  421 CCAGAGTTCT ACTACGATGA GTTTGGTTTC CGTGTGTACA AGGAAGAAGG TGATGAGCCT
  481 GGCTCCAGTC TGCTGGCGAA CTCCCCTCTG ATGGAGGATG CTCCACAGAG GCTGCGGTGG
  541 CAGGCCCACC TGGAGTTCAC CCATAACCAC GATGTGGGGG ATCTCACCTG GGACAAGATT
  601 GCCGTCTCCC TACCCCGCTC TGAGAAGCTC CGCTCCCTGG TGCTGGCCGG CATCCCACAT
  661 GGCATGAGGC CACAGCTGTG GATGCGGCTC TCTGGGGCCC TGCAGAAGAA GAGGAACTCT
  721 GAGCTGTCCT ACCGCGAGAT TGTGAAGAAC AGCTCCAACG ATGAGACCAT CGCTGCCAAG
  781 CAGATCGAGA ANGANCTGCT CGCCNCCATG NNCAGCAACG CCTGCTTCGC CAGCATGGGT
  841 AGCATCGGGG TACCCCGCCT GCGCAGGGTG CTNCGGGNCC TGGCCTGNCT CTACCNAGAG
  901 ATCGGCTACT GCCAGGGCAC CNGNATGGTG NNCGCCTGCC TCCNGCTGNN NNNNGNNGAN
  961 GAGNNNNCCT NNNNGNNGAN GTCTNNCNTC NTNNAGGNNC NGCTCCNNNC CTNCNANTTN
1021 NNNANNNCCN NNNNNGNNNN NCAGANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hiragi, S., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]