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pMRX-bsr-Flag-AID-Rab5A (#RDB19430)

Retroviral expression vector of mouse Rab 5A tagged with FLAG-auxin-inducible degron (AID).

Clone info. Retroviral expression vector of mouse Rab 5A tagged with FLAG-auxin-inducible degron (AID) at N-terminus. See Fig1A of Hatoyama, Y. et al. J. Cell Sci. 2022, 135, jcs.259184.
Comment Expression was confirmed by the depositor with immunoblotting, immunofluorescence
Vector backbone pMRX-IRES-bsr-FLAG (plasmid)
Selectable markers Ampicillin (E. coli), Blasticidin (mammalian cell)
Gene/insert name mouse Rab5a cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hatoyama, Y. et al., J. Cell Sci.(2022), 135, jcs.259184)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hatoyama, Y. et al., J. Cell Sci.(2022), 135, jcs.259184)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19430 pMRX-bsr-Flag-AID-Rab5A Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMRX-bsr-Flag-AID-Rab5A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19430).

Reference section:

Hatoyama, Y., Homma, Y., Hiragi, S., Fukuda, M., Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system. J. Cell Sci. 134 (24): jcs259184 (2021). PMID 34817057. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Hatoyama, Y., Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system. J. Cell Sci. 134 (24): jcs259184 (2021). PMID 34817057. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]