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pMRX-bsr-Flag-AID-Rab5A

Retroviral expression vector of mouse Rab 5A tagged with FLAG-auxin-inducible degron (AID).

Catalog number RDB19430
Resource name pMRX-bsr-Flag-AID-Rab5A
Clone info. Retroviral expression vector of mouse Rab 5A tagged with FLAG-auxin-inducible degron (AID) at N-terminus. See Fig1A of Hatoyama, Y. et al. J. Cell Sci. 2022, 135, jcs.259184.
Comment Expression was confirmed by the depositor with immunoblotting, immunofluorescence
Vector backbone pMRX-IRES-bsr-FLAG (plasmid)
Selectable markers Ampicillin (E. coli), Blasticidin (mammalian cell)
Gene/insert name mouse Rab5a cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab5a
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hatoyama, Y. et al., J. Cell Sci.(2022), 135, jcs.259184)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hatoyama, Y. et al., J. Cell Sci.(2022), 135, jcs.259184)。
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          RDB19430 pMRX-bsr-Flag-AID-Rab5A Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Hatoyama, Y., Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system. J. Cell Sci. 134 (24): jcs259184 (2021). PMID 34817057.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Hatoyama, Y., Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system. J. Cell Sci. 134 (24): jcs259184 (2021). PMID 34817057.
          reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2022.05.16

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