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pMRX-bsr-ss-sfGFP

Retroviral expression vector of a model soluble secretory rotien that consists of a signal sequence (ss) and superfolder GFP.

Catalog number RDB19002
Resource name pMRX-bsr-ss-sfGFP
Clone info. Retroviral expression vector of a model soluble secretory rotien that consists of a signal sequence (ss) and superfolder GFP (sfGFP), a fast-folding derivative of GFP. The signal sequence is derived from mouse calreticulin (Calr).
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pMRX-bsr (plasmid)
Gene/insert name Aequorea victoria GFP cDNA mouse Calr cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
Aequorea victoria GFP
    mouse Calr

          Reference sequence
            
           
          Sequence (full) RDB19002zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Homma, Y. & Fukuda, M. Biochem. Biophys. Res. Commun. 561: 151-157 (2021)).
          Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Homma, Y. & Fukuda, M. Biochem. Biophys. Res. Commun. 561: 151-157 (2021))。
          付加的提供条件 pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19002 pMRX-bsr-ss-sfGFP Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Homma, Y., Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway. Biochem. Biophys. Res. Commun. 561: 151-157 (2021). PMID 34023780.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Homma, Y., Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway. Biochem. Biophys. Res. Commun. 561: 151-157 (2021). PMID 34023780.

          2022.08.12

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