Expression vector of mouse Rab41 constitutive negative mutant (N129I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Clone info. | Expression vector of mouse Rab41 constitutive negative mutant (N129I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay. Rab43 in NCBI |
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Comment | Expression was confirmed by the depositor with yeast-two hybrid assay. |
Vector backbone | pGBD-C1 (plasmid) |
Size of vector backbone | 5.9 kb |
Selectable markers | Ampicillin (E. coli), TRP1 (yeast) |
Gene/insert name | mouse Rab43 cDNA |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021). |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB18919 | pGBD-C1-Rab41-deltaCys-N129I | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pGBD-C1-Rab41-deltaCys-N129I was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18919). |
Reference section:
Osaki, F., Matsui, T., Hiragi, S., Homma, Y., Fukuda, M., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [link to RRC of NBRP] |
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reference | Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP] |
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