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pRetroX-Tet-One-puro-EGFP-2xRBD11 (#RDB18794)

Retroviral expression vector of tetracycline-inducible active Rab11 trapper: mouse tandem Rab11-specific binding domain (2x RBD11) tagged with EGFP.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Osaki, F., J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Retroviral expression vector of tetracycline-inducible active Rab11 trapper: mouse tandem Rab11-specific binding domain (2x RBD11) tagged with EGFP at N-terminus. See Fig5 of Osaki, F. et al. J. Cell Sci. 134 (7) , jcs.257311, 2021.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pRetroX-Tet-ONE-puro (plasmid)
Size of vector backbone 9.5 kb
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Rab11fip2 cDNA mouse Rab11fip4 cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
Remarks Remember that you will be working with samples containing infectious virus.
TET system containing bioresource. Limited Use License must be reviewed before ordering. Please refer Announcement of bioresources containing the tetracycline-inducible gene expression (TET) system.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
TETシステムを用いたバイオリソースです。ご注文の前に Limited Use License を確認していただく必要があります。「テトラサイクリン誘導性遺伝子発現系（TETシステム）を導入したバイオリソースのご利用に関して」をご覧ください。

Catalog # Resource name Shipping form Fee
RDB18794 pRetroX-Tet-One-puro-EGFP-2xRBD11 DNA solution

How to cite this biological resource

Materials & Methods section:

The pRetroX-Tet-One-puro-EGFP-2xRBD11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18794).

Reference section:

Osaki, F., Matsui, T., Hiragi, S., Homma, Y., Fukuda, M., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [PubMed] [Article] [RRC of NBRP]