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pMRX-puro-EGFP-FM-RBD11-mut

Retroviral expression vector of negative control of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) mutant tagged with EGFP.

Catalog number RDB18793
Resource name pMRX-puro-EGFP-FM-RBD11-mut
Clone info. Retroviral expression vector of negative control of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) mutant tagged with EGFP at N-terminus. See Fig5 of Osaki, F. et al. J. Cell Sci. 134 (7) , jcs.257311, 2021. FM is an artificial oligomerization domain derived form mouse FKBP12 (see Hirano, S. et al. Autophagy, 12 (2), 312-326, 2016.)
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-puro (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name mouse Rab11fip2 cDNA mouse Rab11fip4 cDNA mouse Fkbp1a cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab11fip2 (NCBI Gene 74998) | mouse Rab11fip4 (NCBI Gene 268451) | mouse Fkbp1a (NCBI Gene 14225) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
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RDB18793 pMRX-puro-EGFP-FM-RBD11-mut Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

2021.08.13

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