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pMRX-puro-EGFP-FM-RBD11-mut (#RDB18793)

Retroviral expression vector of negative control of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) mutant tagged with EGFP.

Clone info. Retroviral expression vector of negative control of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) mutant tagged with EGFP at N-terminus. See Fig5 of Osaki, F. et al. J. Cell Sci. 134 (7) , jcs.257311, 2021. FM is an artificial oligomerization domain derived form mouse FKBP12 (see Hirano, S. et al. Autophagy, 12 (2), 312-326, 2016.)
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-puro (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Rab11fip2 cDNA mouse Rab11fip4 cDNA mouse Fkbp1a cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB18793hts01.seq checkAssembled from experimentally sequenced data.
Publication Osaki, F., J. Cell Sci. 134 (7): jcs257311 (2021). [link to RRC of NBRP]
Test sheet RDB18793_B1Dup1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18793 pMRX-puro-EGFP-FM-RBD11-mut DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMRX-puro-EGFP-FM-RBD11-mut was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18793).

Reference section:

Osaki, F., Matsui, T., Hiragi, S., Homma, Y., Fukuda, M., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [link to RRC of NBRP]