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pMRX-puro-EGFP-FM-RBD11

Retroviral expression vector of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) tagged with EGFP.

Catalog number RDB18792
Resource name pMRX-puro-EGFP-FM-RBD11
Clone info. Retroviral expression vector of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) tagged with EGFP at N-terminus. See Fig5 of Osaki, F. et al. J. Cell Sci. 134 (7) , jcs.257311, 2021. FM is an artificial oligomerization domain derived form mouse FKBP12 (see Hirano, S. et al. Autophagy, 12 (2), 312-326, 2016.)
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-puro (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name mouse Rab11fip2 cDNA mouse Rab11fip4 cDNA mouse Fkbp1a cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab11fip2 mouse Rab11fip4 mouse Fkbp1a

      Reference sequence
        

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
      Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
      Ordering Please visit Information of Request for Distribution.[link] 
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      Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
      This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
      Remarks Remember that you will be working with samples containing infectious virus.
      提供案内 (日本国内) [open/close]

      提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。
      付加的提供条件 pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
      提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
      提供依頼書 [Word]
      提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
      GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
      備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

      Catalog # Resource name Availability Shipping form Fee (non-profit org.)
      RDB18792 pMRX-puro-EGFP-FM-RBD11 Under QC test. Please contact us. DNA solution

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      References and tips

      Electronic file

      Original reference

      original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

      Further references such as user reports and related articles (go to bottom)

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      Sequence information

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


      References

      Original, user report and related articles

      original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

      2022.05.16

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