RDB18792 - pMRX-puro-EGFP-FM-RBD11

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pMRX-puro-EGFP-FM-RBD11

Retroviral expression vector of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) tagged with EGFP.


Catalog number	RDB18792
Resource name	pMRX-puro-EGFP-FM-RBD11
Clone info.	Retroviral expression vector of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) tagged with EGFP at N-terminus. See Fig5 of Osaki, F. et al. J. Cell Sci. 134 (7) , jcs.257311, 2021. FM is an artificial oligomerization domain derived form mouse FKBP12 (see Hirano, S. et al. Autophagy, 12 (2), 312-326, 2016.) Expression was confirmed by the depositor with immunoblotting.
Vector backbone	pMRX-puro (plasmid)
Selectable markers	Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name	mouse Rab11fip2 cDNA mouse Rab11fip4 cDNA mouse Fkbp1a cDNA
Depositor\|Developer	Fukuda, Mitsunori \|
Other clones in our bank	mouse Rab11fip2 (NCBI Gene 74998) \| mouse Rab11fip4 (NCBI Gene 268451) \| mouse Fkbp1a (NCBI Gene 14225) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).

Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.

Ordering Please visit Information of Request for Distribution.[link]
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]

Remarks Remember that you will be working with samples containing infectious virus.
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
Additional terms and conditions for distribution	Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Remember that you will be working with samples containing infectious virus. Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。
付加的提供条件	pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB18792	pMRX-puro-EGFP-FM-RBD11	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original	Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

Further references such as user reports and related articles (go to bottom)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

2021.08.13

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