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pMRX-puro-EGFP-RBD11

Retroviral expression vector for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) tagged with EGFP.

Catalog number RDB18788
Resource name pMRX-puro-EGFP-RBD11
Clone info. Retroviral expression vector for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) tagged with EGFP at N-terminus,. See Fig3 of Osaki, F. et al. J. Cell Sci. 134 (7) , jcs.257311, 2021.
Expression was confirmed by the depositor with Immunofluorescence.
Vector backbone pMRX-puro (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name mouse Rab11fip2 cDNA mouse Rab11fip4 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab11fip2 (NCBI Gene 74998) | mouse Rab11fip4 (NCBI Gene 268451) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
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Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18788 pMRX-puro-EGFP-RBD11 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

2021.08.13

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