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pEF-HA-M-INK

M-INK, a tool for tagging mature melanosomes.

Catalog number RDB18649
Resource name pEF-HA-M-INK
Clone info. M-INK, a tool for tagging mature melanosomes. Expression vector of mouse kinesin family member 1C (Kif1c) tagged with HA at N-terminus.
Visullization of melanosomes/melanin in skin samples. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-HA (plasmid )
Selectable markers Ampicillin
Gene/insert name Mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. Sci. Rep. 5, 8238, 2015); (Yoshikawa-Murakami et al. Int. J. Mol. Sci. 21, 8514, 2020 (doi: 10.3390/ ijms21228514))
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18649 pEF-HA-M-INK Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB18649pka01.seq

Original reference

original Yoshikawa-Murakami, C., A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues. Int. J Mol. Sci. 21 (22): 8514 (2020). PMID 33198186.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Yoshikawa-Murakami, C., A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues. Int. J Mol. Sci. 21 (22): 8514 (2020). PMID 33198186.
reference Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263.

2021.02.05

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