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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-HA-M-INK

M-INK, a tool for tagging mature melanosomes.

Catalog number RDB18649
Resource name pEF-HA-M-INK
Clone info. M-INK, a tool for tagging mature melanosomes. Expression vector of mouse kinesin family member 1C (Kif1c) tagged with HA at N-terminus.
Comment Visullization of melanosomes/melanin in skin samples. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-HA (plasmid )
Selectable markers Ampicillin
Gene/insert name Mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
Mouse Kif1c

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. Sci. Rep. 5, 8238, 2015); (Yoshikawa-Murakami et al. Int. J. Mol. Sci. 21, 8514, 2020 (doi: 10.3390/ ijms21228514))
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al. Sci. Rep. 5, 8238, 2015); (Yoshikawa-Murakami et al. Int. J. Mol. Sci. 21, 8514, 2020 (doi: 10.3390/ ijms21228514))。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18649 pEF-HA-M-INK DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (partial) RDB18649pka01.seq provided by the depositor

          Original reference

          original Yoshikawa-Murakami, C., A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues. Int. J Mol. Sci. 21 (22): 8514 (2020). PMID 33198186.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualization of Organelles (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18649_B0Lmp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: HA, insert 5'
          Sequence file: RDB18649_B0Lma.seq check
          >18649_18649_B1IlF_EF1a-C_F_A02_01_ABI08.ab1
              1 CNNTNNNGAN TTTGGNNCTT GGTTNNTTCT CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT
             61 TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT CGATTCCACC
            121 ATGGGTTACC CATACGACGT CCCAGACTAC GCTGGATCTG ATGATAATGA CGAGTCTGGT
            181 TTGGTCACCT GGGCCCCACC GGAAGGGCCA GAAGCAGTAG AGGAGACAGT GCCCAACGAC
            241 CACTCACCAG CTGTCCGGCC CACCTCACCA CCTCTGTCCA GCTGGGAGCG GGTGTCGAGA
            301 TTGATGGAAG AGGACCCTGC CTTCCGTCGC GGCCGCCTTC GCTGGCTCAA GCAGGAGCAG
            361 CTGCGGCTGC AGGGACTGCA GGGCGCTGGG GGCCGGGGTG GGGGACTGCG CAGGCCTCCA
            421 GCCCGCTTTG TGCCCCCGCA TGATTGCAAG CTGCGCTTCC CCTTCAAAAG CAACCCTCAG
            481 CACAGGGAGT CCTGGCCAGG AATGGGGAGT GGAGAGGCCC CAGCTCCTCA GCCCCCTGAA
            541 GAGGTCACTG TCCCCCCAGC ACCCCCTAAC CGTANGCCTC CAAGTCCCCG AAGANCCCAC
            601 CGTTCTCGCA NGANTTCCCT AGATGGAGGG AGCCGATCTC NGGGNNNGGG GTTCTACNCA
            661 GCCNGAACCC CNGCACTTAN NGNCCCAAAA GCANNANNNT TATCCCNNNN NGCCCNAGCC
          721 NNGCCNNGCC NNNNNGNNNN GNNCCNNNNN NCCNNNCNNN NNNNNNN
          //
          Primer: hGCSF_R
          Region: insert 3'
          Sequence file: RDB18649_B0Lmb.seq check
          >18649_18649_B1IlF_hGCSF_R_D02_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNTGGGG NNCNNNNNTC TAGANNCGGC CGCGAATTCA
             61 CTAGTGATTT CACACAGCTG CCCCACTCTC TTTGAGGTCA GGGGCTGAGC GCTGCCGTCG
            121 CATTCGCGGA GGGGTAGTGT ATGGTGGGTA GCGGGGCCCT GGCCGCTGGG CTGGGCTGGG
            181 CTGGGGCTGT TGGGGATAAC CGTTGTGCTT TTGGGGCCGT AAGTGCTGGG GTTCTGGCTG
            241 TGTAGAACCC CCTCCCCGAG ATCGGCTCCC TCCATCTAGG GAATTCCTGC GAGAACGGTG
            301 GGGTCTTCGG GGACTTGGAG GCCTACGGTT AGGGGGTGCT GGGGGGACAG TGACCTCTTC
            361 AGGGGGCTGA GGAGCTGGGG CCTCTCCACT CCCCATTCCT GGCCAGGACT CCCTGTGCTG
            421 AGGGTTGCTT TTGAAGGGGA AGCGCAGCTT GCAATCATGC GGGGGCACAA AGCGGGCNGN
          481 ANGCCTGCGC ANNCCCCCAC CCCGGCCCCN NNNNCCCTNN NNNNNNNN
          //
          Primer: pUC-CAPbs_up_F
          Region: SV40 pro_ori
          Sequence file: RDB18649_B0Lmc.seq check
          >18649_18649_B1IlF_pUC-CAPbs__up_F_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA
             61 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG
            121 AAACAGCTAT GACCATGATT ACGCCAAGCT TAATTCCCTC CCCAGCAGGC AGAAGTATGC
            181 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC
            241 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT
            301 ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT
            361 TTGGAGGCCT AGGCTTTTGC AAAAAGCTTT GCAAAGATGG ATAAAGTTTT AAACAGAGAG
            421 GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA GTGGGAATTG GCTCCGGTGC
            481 CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GANGGGTCGG
            541 CAATTGAACC GGTGCCNANA GAANGNGGCG CGGGGTAAAC TGGGAAAGTG ANGTCGNGNA
            601 NTGNNNNNNC CNTTTTCCNN NNGNNGGGGG ANNANNNNAN NNANNGNANN ANTCNNNNNN
          661 NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yoshikawa-Murakami, C., A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues. Int. J Mol. Sci. 21 (22): 8514 (2020). PMID 33198186.
          reference Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263.

          2022.05.16

          GNP_filter3_RDBDEP_html_220516.pl