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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-HA-M-INK (#RDB18649)

M-INK, a tool for tagging mature melanosomes.

Clone info. M-INK, a tool for tagging mature melanosomes. Expression vector of mouse kinesin family member 1C (Kif1c) tagged with HA at N-terminus.
Comment Visullization of melanosomes/melanin in skin samples. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-HA (plasmid )
Selectable markers Ampicillin
Gene/insert name Mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (partial) RDB18649pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. Sci. Rep. 5, 8238, 2015); (Yoshikawa-Murakami et al. Int. J. Mol. Sci. 21, 8514, 2020 (doi: 10.3390/ ijms21228514))
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al. Sci. Rep. 5, 8238, 2015); (Yoshikawa-Murakami et al. Int. J. Mol. Sci. 21, 8514, 2020 (doi: 10.3390/ ijms21228514))。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18649 pEF-HA-M-INK DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-HA-M-INK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18649).

Reference section:

Yoshikawa-Murakami, C., Mizutani, Y., Ryu, A., Naru, E., Teramura, T., Homma, Y., Fukuda, M., A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues. Int. J Mol. Sci. 21 (22): 8514 (2020). PMID 33198186. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18649_B0Lmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: HA, insert 5'
Sequence file: RDB18649_B0Lma.seq check
>18649_18649_B1IlF_EF1a-C_F_A02_01_ABI08.ab1
    1 CNNTNNNGAN TTTGGNNCTT GGTTNNTTCT CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT
   61 TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT CGATTCCACC
  121 ATGGGTTACC CATACGACGT CCCAGACTAC GCTGGATCTG ATGATAATGA CGAGTCTGGT
  181 TTGGTCACCT GGGCCCCACC GGAAGGGCCA GAAGCAGTAG AGGAGACAGT GCCCAACGAC
  241 CACTCACCAG CTGTCCGGCC CACCTCACCA CCTCTGTCCA GCTGGGAGCG GGTGTCGAGA
  301 TTGATGGAAG AGGACCCTGC CTTCCGTCGC GGCCGCCTTC GCTGGCTCAA GCAGGAGCAG
  361 CTGCGGCTGC AGGGACTGCA GGGCGCTGGG GGCCGGGGTG GGGGACTGCG CAGGCCTCCA
  421 GCCCGCTTTG TGCCCCCGCA TGATTGCAAG CTGCGCTTCC CCTTCAAAAG CAACCCTCAG
  481 CACAGGGAGT CCTGGCCAGG AATGGGGAGT GGAGAGGCCC CAGCTCCTCA GCCCCCTGAA
  541 GAGGTCACTG TCCCCCCAGC ACCCCCTAAC CGTANGCCTC CAAGTCCCCG AAGANCCCAC
  601 CGTTCTCGCA NGANTTCCCT AGATGGAGGG AGCCGATCTC NGGGNNNGGG GTTCTACNCA
  661 GCCNGAACCC CNGCACTTAN NGNCCCAAAA GCANNANNNT TATCCCNNNN NGCCCNAGCC
721 NNGCCNNGCC NNNNNGNNNN GNNCCNNNNN NCCNNNCNNN NNNNNNN
//
Primer: hGCSF_R (Pr0031)
Region: insert 3'
Sequence file: RDB18649_B0Lmb.seq check
>18649_18649_B1IlF_hGCSF_R_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGGGG NNCNNNNNTC TAGANNCGGC CGCGAATTCA
   61 CTAGTGATTT CACACAGCTG CCCCACTCTC TTTGAGGTCA GGGGCTGAGC GCTGCCGTCG
  121 CATTCGCGGA GGGGTAGTGT ATGGTGGGTA GCGGGGCCCT GGCCGCTGGG CTGGGCTGGG
  181 CTGGGGCTGT TGGGGATAAC CGTTGTGCTT TTGGGGCCGT AAGTGCTGGG GTTCTGGCTG
  241 TGTAGAACCC CCTCCCCGAG ATCGGCTCCC TCCATCTAGG GAATTCCTGC GAGAACGGTG
  301 GGGTCTTCGG GGACTTGGAG GCCTACGGTT AGGGGGTGCT GGGGGGACAG TGACCTCTTC
  361 AGGGGGCTGA GGAGCTGGGG CCTCTCCACT CCCCATTCCT GGCCAGGACT CCCTGTGCTG
  421 AGGGTTGCTT TTGAAGGGGA AGCGCAGCTT GCAATCATGC GGGGGCACAA AGCGGGCNGN
481 ANGCCTGCGC ANNCCCCCAC CCCGGCCCCN NNNNCCCTNN NNNNNNNN
//
Primer: pUC-CAPbs_up_F (Pr0536)
Region: SV40 pro_ori
Sequence file: RDB18649_B0Lmc.seq check
>18649_18649_B1IlF_pUC-CAPbs__up_F_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA
   61 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG
  121 AAACAGCTAT GACCATGATT ACGCCAAGCT TAATTCCCTC CCCAGCAGGC AGAAGTATGC
  181 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC
  241 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT
  301 ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT
  361 TTGGAGGCCT AGGCTTTTGC AAAAAGCTTT GCAAAGATGG ATAAAGTTTT AAACAGAGAG
  421 GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA GTGGGAATTG GCTCCGGTGC
  481 CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GANGGGTCGG
  541 CAATTGAACC GGTGCCNANA GAANGNGGCG CGGGGTAAAC TGGGAAAGTG ANGTCGNGNA
  601 NTGNNNNNNC CNTTTTCCNN NNGNNGGGGG ANNANNNNAN NNANNGNANN ANTCNNNNNN
661 NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshikawa-Murakami, C., A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues. Int. J Mol. Sci. 21 (22): 8514 (2020). PMID 33198186. [link to RRC of NBRP]
reference Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263. [link to RRC of NBRP]